Molecular dynamics simulations of ternary lipid bilayers containing plant sterol and glucosylceramide

Emami, Shiva; Azadmard‐Damirchi, Sodeif; Peighambardoust, Seyed Hadi; Hesari, Javad; Valizadeh, Hadi; Faller, Roland

doi:10.1016/j.chemphyslip.2017.01.003

Cited by 14 publications

(6 citation statements)

References 57 publications

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“…In the absence of HPOs, GluCer-sitosterol enriched domains are generated within the PLPC matrix in quite accordance with previous molecular dynamics study [50]. The presence of each HPO influences very differently the lateral reorganization of the domains.…”

Section: Interaction Of Hpos With Ppm-mimicking Modelssupporting

confidence: 90%

Linoleic and linolenic acid hydroperoxides interact differentially with biomimetic plant membranes in a lipid specific manner

Deleu

Deboever

Nasir

et al. 2019

Colloids and Surfaces B: Biointerfaces

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Section: Interaction Of Hpos With Ppm-mimicking Modelssupporting

confidence: 90%

Linoleic and linolenic acid hydroperoxides interact differentially with biomimetic plant membranes in a lipid specific manner

Deleu

Deboever

Nasir

et al. 2019

Colloids and Surfaces B: Biointerfaces

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“…The NI-LNs were five-nm high and about 50 nm in diameter (Figure 2B), which was consistent with the cryo-TEM images. The thickness of a lipid bilayer is four to six nm [40,41]; thus, the NI-LNs were a single lipid bilayer. Spherical vesicles such as liposomes sometimes strongly adsorbed onto the substrate, resulting in heights over 10 nm that indicated double lipid bilayers [42].…”

Section: Resultsmentioning

confidence: 99%

Combination of Roll Grinding and High-Pressure Homogenization Can Prepare Stable Bicelles for Drug Delivery

et al. 2018

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To improve the solubility of the drug nifedipine (NI), NI-encapsulated lipid-based nanoparticles (NI-LNs) have been prepared from neutral hydrogenated soybean phosphatidylcholine and negatively charged dipalmitoylphosphatidylglycerol at a molar ratio of 5/1 using by roll grinding and high-pressure homogenization. The NI-LNs exhibited high entrapment efficiency, long-term stability, and enhanced NI bioavailability. To better understand their structures, cryo transmission electron microscopy and atomic force microscopy were performed in the present study. Imaging from both instruments revealed that the NI-LNs were bicelles. Structures prepared with a different drug (phenytoin) or with phospholipids (dimyristoylphosphatidylcholine, dipalmitoylphosphatidylcholine, and distearoylphosphatidylcholine) were also bicelles. Long-term storage, freeze-drying, and high-pressure homogenization did not affect the structures; however, different lipid ratios, or the presence of cholesterol, did result in liposomes (5/0) or micelles (0/5) with different physicochemical properties and stabilities. Considering the result of long-term stability, standard NI-LN bicelles (5/1) showed the most long-term stabilities, providing a useful preparation method for stable bicelles for drug delivery.

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“…The use of sitosterol or stigmasterol in research involving plant membranes and membrane-associated proteins is recommended ( Zhuang et al, 2017 ) to recapitulate the fluidity and thermotropic properties of model membranes ( Jovanović et al, 2019 ). Sitosterol, as is the case for cholesterol in mammal membranes, contributes to packing the lipid bilayer more tightly into a liquid ordered phase ( Emami et al, 2017 ), and this aspect is important in MD simulation to attain equilibration more effectively ( Silva et al, 2011 ). Myristic acid was chosen as a representative fatty acid for computational tractability.…”

Section: Methodsmentioning

confidence: 99%

The structure and flexibility analysis of the Arabidopsis synaptotagmin 1 reveal the basis of its regulation at membrane contact sites

et al. 2021

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Non-vesicular lipid transfer at ER and plasma membrane (PM) contact sites (CS) is crucial for the maintenance of membrane lipid homeostasis. Extended synaptotagmins (E-Syts) play a central role in this process as they act as molecular tethers of ER and PM and as lipid transfer proteins between these organelles. E-Syts are proteins constitutively anchored to the ER through an N-terminal hydrophobic segment and bind the PM via a variable number of C-terminal C2 domains. Synaptotagmins (SYTs) are the plant orthologous of E-Syts and regulate the ER–PM communication in response to abiotic stress. Combining different structural and biochemical techniques, we demonstrate that the binding of SYT1 to lipids occurs through a Ca2+-dependent lipid-binding site and by a site for phosphorylated forms of phosphatidylinositol, thus integrating two different molecular signals in response to stress. In addition, we show that SYT1 displays three highly flexible hinge points that provide conformational freedom to facilitate lipid extraction, protein loading, and subsequent transfer between PM and ER.

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Molecular dynamics simulations of ternary lipid bilayers containing plant sterol and glucosylceramide

Cited by 14 publications

References 57 publications

Linoleic and linolenic acid hydroperoxides interact differentially with biomimetic plant membranes in a lipid specific manner

Linoleic and linolenic acid hydroperoxides interact differentially with biomimetic plant membranes in a lipid specific manner

Combination of Roll Grinding and High-Pressure Homogenization Can Prepare Stable Bicelles for Drug Delivery

The structure and flexibility analysis of the Arabidopsis synaptotagmin 1 reveal the basis of its regulation at membrane contact sites

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