Molecular cloning, characterization and expression analysis of two juvenile hormone esterase-like carboxylesterase cDNAs in Chinese mitten crab, Eriocheir sinensis

Genomic Characterization and Expression of Juvenile Hormone Esterase-Like Carboxylesterase Genes in Pacific White Shrimp, Litopenaeus vannamei

“…It indicated that LvCXE5 was not an ortholog of insect JHE. Meanwhile, previous studies found that CXE genes containing the GESAG motif were proposed to have esterase activity for MF in crustaceans [30,32]. Consistently, 14 LvCXEs contained GESAG in our study.…”

“…However, due to the weakness of the experimental platform, the study on the function of juvenile hormone esterase-like (JHE-like) carboxylesterases (CXE) in crustaceans is limited. Like insects, CXE was supposed to degrade MF through ester hydrolysis in crustaceans, and it was supported by the results that the expression of CXE genes was up-regulated by eyestalk ablation or MF treatment in several crustacean species [30][31][32][33]. Nevertheless, they mainly focused on the role of CXE in reproductive development and the innate immune response [30][31][32][33], whereas their functions in early development and the molting process are barely known in crustaceans.…”

Molecular cloning and expression pattern of the juvenile hormone epoxide hydrolase gene from the giant freshwater prawn Macrobrachium rosenbergii during larval development and the moult cycle

“…JH degradation pathway family members like juvenile hormone epoxide hydrolase (JHEH), juvenile hormone esterase (JHE) and juvenile hormone esterase binding protein (JHEBP) were first detected in the shrimp Neocaridina denticulata (Sin et al, 2015). Similarly, Juvenile hormone esterase-like carboxylesterase genes were found in L. vannamei, Eriocheir sinensis (Xu et al, 2017;Zhang et al, 2020). In this study, we characterized JHEH derived from M. rosenbergii.…”

“…Similarly, Juvenile hormone esterase‐like carboxylesterase genes were found in L . vannamei , Eriocheir sinensis (Xu et al, 2017; Zhang et al, 2020). In this study, we characterized JHEH derived from M .…”

“…Previous studies have shown that MF and ecdysteroids are suppressively controlled by mandibular organ‐inhibiting hormone (MOIH) secreted from the X‐organ‐sinus gland complex in eyestalks (Hydeet al, 2019; Nakatsuji et al, 2009). Additionally, eyestalk ablation of crustacean resulted in the induction of JH metabolic genes (Xu et al, 2017; Zhu et al, 2018). Upon ablation of the eyestalk, these inhibitory effects of these neuropeptides on downstream target genes will be released.…”