Surface charge-bias impact of amine-contained pseudozwitterionic biointerfaces on the human blood compatibility

Venault, Antoine; Hsu, Ko-Jen; Yeh, Lu-Chen; Chinnathambi, Arunachalam; Ho, Hsin‐Tsung; Chang, Yung

doi:10.1016/j.colsurfb.2016.12.040

Cited by 21 publications

(21 citation statements)

References 62 publications

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“…It has been reported that nanoparticles with a relatively high surface charge could generate repulsion between the particles. In addition, nanoparticles with small particle size and negative surface charge exhibit preferential uptake by biological membrane, which could be beneficial for larger contact areas and electrostatic interaction 47,48Figure 2Characterization of P-TBESD.…”

Section: Resultsmentioning

confidence: 99%

Proliposomes for oral delivery of total biflavonoids extract from Selaginella doederleinii: formulation development, optimization, and in vitro–in vivo characterization

Chen¹,

Wang²,

Lin³

et al. 2019

IJN

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PurposeAmentoflavone, robustaflavone, 2’’,3’’-dihydro-3’,3’’’-biapigenin, 3’,3’’’-binaringenin and delicaflavone are five major active ingredients in the total biflavonoids extract from Selaginella doederleinii (TBESD) with favorable anticancer properties. However, the natural-derived potent antitumor agent of TBESD is undesirable due to its poor solubility. The present study was to develop and optimize a proliposomal formulation of TBESD (P-TBESD) to improve its solubility, oral bioavailability and efficacy.Materials and methodsP-TBESD containing a bile salt, a protective hydrophilic isomalto-oligosaccharides (IMOs) coating, were successfully prepared by thin film dispersion-sonication method. The physicochemical and pharmacokinetic properties of P-TBESD were characterized, and the antitumor effect was evaluated using the HT-29 xenograft-bearing mice models in rats.ResultsCompared with TBESD, the relative bioavailability of amentoflavone, robustaflavone, 2’’,3’’-dihydro-3’,3’’’-biapigenin, 3’,3’’’-binaringenin and delicaflavone from P-TBESD were 669%, 523%, 761%, 955% and 191%, respectively. The results of pharmacodynamics demonstrated that both TBESD and P-TBESD groups afforded antitumor effect without systemic toxicity, and the antitumor effect of P-TBESD was significantly superior to that of raw TBESD, based on the tumor growth inhibition and histopathological examination.ConclusionHence, IMOs-modified proliposomes have promising potential for TBESD solving the problem of its poor solubility and oral bioavailability, which can serve as a practical oral preparation for TBESD in the future cancer therapy.

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Section: Resultsmentioning

confidence: 99%

Proliposomes for oral delivery of total biflavonoids extract from Selaginella doederleinii: formulation development, optimization, and in vitro–in vivo characterization

Chen¹,

Wang²,

Lin³

et al. 2019

IJN

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“…The oil-in-water diiodomethane contact angles of the hydrogels were found to be in the range of 130−145°( Supporting Information), showing overall superhydrophilicity without a clear correlation to the CA/DMAEMA ratios; this was also observed in a similar study. 26 The next characterization was to determine the swelling characteristics of the CA/ DMAEMA hydrogels in both DI water and PBS buffer. The results were also shown in Figure 2.…”

Section: Resultsmentioning

confidence: 99%

Bioinspired Pseudozwitterionic Hydrogels with Bioactive Enzyme Immobilization via pH-Responsive Regulation

et al. 2018

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This is an author's version published in: http://oatao.univ-toulouse.fr/24260 ABSTRACT: Hydrogels are hydrated networks of flexible polymers with versatile biomedical applications, and their resistance to nonspecific protein adsorption is critical. On the other hand, functionalization with other biomacromolecules would greatly enhance their biotechnological potential. The aim of this research is to prepare low fouling hydrogel polymers for selective protein immobilization. Initially, hydrogels were prepared by controlling the composition ratios of 2-carboxyethyl acrylate (CA) and 2-dimethylaminoethyl methacrylate (DMAEMA) monomers in an N,Nmethylene-bis-acrylamide (NMBA) cross-linked free radical polymerization reaction. This series of hydrogels (C1D9 to C9D1) were then analyzed by X-ray photoelectron spectroscopy (XPS) and dynamic laser scattering to confirm the actual polymer ratios and surface charge. When the composition ratio was set at CA:6 vs DMEAMA:4 (C6D4), the hydrogel showed nearly neutral surface charge and an equivalent reaction ratio of CA vs DMAEMA in the hydrogel. Subsequent analysis showed excellent antifouling properties, low blood cell adhesion, hemocompatibility, and platelet deactivation. Moreover, this hydrogel exhibited pH responsiveness to protein adsorption and was then used to facilitate the immobilization of lipase as an indication of active protein functionalization while still maintaining a low fouling status. In summary, a mixed-charge nonfouling pseudozwitterionic hydrogel could be prepared, and its pH-responsive adsorption holds potential for designing a biocompatible tissue engineering matrix or membrane enzyme reactors.

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“…The results of Figure 4 indicate that the G20-S40-D40-grafted PDMS surface is neutral at pH 7.4 while the surface grafted with G20-S55-D25 bears a net negative charge at pH 7.4 (−7.7 mV), and previous research showed that blood cells had a higher tendency to interact with negative surfaces than with a neutral and hydrophilic one, despite their net negative charge. 47 Effect of the Copolymer Composition on the Attachment/Detachment of Bacteria on/from the Grafted-PDMS Surfaces: Evaluation of the Self-Cleaning Ability of the Grafted Surfaces. To evaluate the self-cleaning performance of poly(GMA-co-SBMA-co-DMAEMA), an ideal polymer ratio that can both exhibit excellent fouling resistance and pH dependency should be identified.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Self-Cleaning Interfaces of Polydimethylsiloxane Grafted with pH-Responsive Zwitterionic Copolymers

et al. 2018

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Self-cleaning surfaces allow the reversible attachment and detachment of microorganisms which show great promise in regards to their reusability as smart biomaterials. However, a widely used biomaterial such as polydimethylsiloxane (PDMS) suffers from high biofouling activity and hydrophobic recovery that results in decreased efficiency and stability. A current challenge is to modify and fabricate self-cleaning PDMS surfaces by incorporating antifouling and pH-sensitive properties. To address this, we synthesized a zwitterionic and pH-sensitive random poly(glycidyl methacrylate- co-sulfobetaine methacrylate- co-2-(dimethylamino)ethyl methacrylate) polymer, poly(GMA- co-SBMA- co-DMAEMA). In this work, chemical modification of PDMS was done by grafting onto poly(GMA- co-SBMA- co-DMAEMA) after surface activation via UV and ozone for 90 min to ensure the formation of covalent bonds necessary for stable grafting. The PDMS grafted with G20-S40-D40 exhibit antifouling and pH-sensitive properties by mitigating fibrinogen adsorption, blood cell adhesion, and releasing 98% adhered E. coli bacteria after immersion at basic pH. The grafting of poly(GMA- co-SBMA- co-DMAEMA) presented in this work shows attractive potential for biomedical and industrial applications as a simple, smart, and effective method for the modification of PDMS interfaces.

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Surface charge-bias impact of amine-contained pseudozwitterionic biointerfaces on the human blood compatibility

Cited by 21 publications

References 62 publications

<p>Proliposomes for oral delivery of total biflavonoids extract from <em>Selaginella doederleinii</em>: formulation development, optimization, and in vitro–in vivo characterization</p>

<p>Proliposomes for oral delivery of total biflavonoids extract from <em>Selaginella doederleinii</em>: formulation development, optimization, and in vitro–in vivo characterization</p>

Bioinspired Pseudozwitterionic Hydrogels with Bioactive Enzyme Immobilization via pH-Responsive Regulation

Self-Cleaning Interfaces of Polydimethylsiloxane Grafted with pH-Responsive Zwitterionic Copolymers

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