Control of meiotic pairing and recombination by chromosomally tethered 26
            <i>S</i>
            proteasome

Ahuja, Jasvinder S.; Sandhu, Rima; Rana, Mainpal; Lawson, Crystal; Henley, Hanna; Hunt, Patricia A.; Yanowitz, Judith L.; Börner, G. Valentin

doi:10.1126/science.aaf4778

Cited by 82 publications

(117 citation statements)

References 42 publications

(68 reference statements)

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“…Therefore, it is clear that MOF and ZYGO1 play distinct roles during meiosis in rice. Interestingly, recent studies reported that chromosomally tethered proteasomes promote pairing and recombination in mouse, budding yeast, and C. elegans, suggesting that the meiotic programmed degradation event is highly conserved among species (Ahuja et al, 2017;Rao et al, 2017). We also found that ZYGO1 shows limited sequence similarity to FBL12 in mouse.…”

Section: Zygo1 Promotes Bouquet Formation Within An Scf Complexmentioning

confidence: 49%

The F-Box Protein ZYGO1 Mediates Bouquet Formation to Promote Homologous Pairing, Synapsis, and Recombination in Rice Meiosis

et al. 2017

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Telomere bouquet formation, a highly conserved meiotic event, plays an important role in homologous pairing and therefore progression of meiosis; however, the underlying molecular mechanism remains largely unknown. Here, we identified ZYGOTENE1 (ZYGO1), a novel F-box protein in rice (Oryza sativa), and verified its essential role in bouquet formation during early meiosis. In zygo1 mutants, zygotene chromosome aggregation and telomere clustering failed to occur. The suppressed telomere clustering in homologous pairing aberration in rice meiosis1 (pair1) zygo1 and rice completion of meiotic recombination (Oscom1) zygo1 double mutants, together with the altered localization of OsSAD1 (a SUN protein associated with the nuclear envelope) in zygo1, showed that ZYGO1 has a significant function in bouquet formation. In addition, the interaction between ZYGO1 and rice SKP1-like protein 1 suggested that ZYGO1 might modulate bouquet formation as a component of the SKP1-Cullin1-F-box complex. Although double-strand break formation and early recombination element installation occurred normally, zygo1 mutants showed defects in full-length pairing and synaptonemal complex assembly. Furthermore, crossover (CO) formation was disturbed, and foci of Human enhancer of invasion 10 were restricted to the partially synapsed chromosome regions, indicating that CO reduction might be caused by the failure of full-length chromosome alignment in zygo1. Therefore, we propose that ZYGO1 mediates bouquet formation to efficiently promote homolog pairing, synapsis, and CO formation in rice meiosis.

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Section: Zygo1 Promotes Bouquet Formation Within An Scf Complexmentioning

confidence: 49%

The F-Box Protein ZYGO1 Mediates Bouquet Formation to Promote Homologous Pairing, Synapsis, and Recombination in Rice Meiosis

et al. 2017

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“…Alternatively, the binding and release of additional proteins to the SC could also function in this manner. For example, the recent discovery that the proteasome can be associated with synapsed chromosomes [41] provides an attractive model for SC destabilization. We envision two models to explain how desynapsis can be achieved.…”

Section: Discussionmentioning

confidence: 99%

A Surveillance System Ensures Crossover Formation in C. elegans

et al. 2016

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SUMMARY Crossover (CO) recombination creates a physical connection between homologs that promotes their proper segregation at meiosis I (MI). Failure to realize an obligate CO causes homologs to attach independently to the MI spindle and separate randomly, leading to nondisjunction. However, mechanisms that determine whether homolog pairs have received crossovers remain mysterious. We describe a surveillance system in C. elegans that monitors recombination intermediates and couples their formation to meiotic progression. Recombination intermediates are required to activate the system that then delays further processing if crossover precursors are lacking on even one chromosome. The synaptonemal complex, a specialized, proteinaceous structure connecting homologous chromosomes, is stabilized in cis on chromosomes that receive a crossover and destabilized on those lacking crossovers, a process that is dependent on the function of the polo-like kinase, PLK-2. These results reveal a new layer of communication between crossover-committed intermediates and the synaptonemal complex that functions as a cis-acting, obligate, crossover-counting mechanism.

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“…In mammals, every chromosome pair obtains many recombination sites but generally yields only one to two crossover sites (Kauppi et al, 2004). Designations of which recombination sites become crossovers involve antagonistic roles between ubiquitin E3 ligase HEI10 and SUMO E3 ligase RNF212 (Reynolds et al, 2013;Qiao et al, 2014;Rao et al, 2017;Ahuja et al, 2017). It is possible that the SMC5/6 complex is a substrate of HEI10 and RNF212.…”

Section: Smc5/6 Localization Pattern Implicates Multiple Functions Dumentioning

confidence: 99%

SMC5/6 is required for the formation of segregation-competent bivalent chromosomes during meiosis I in mouse oocytes

et al. 2017

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SMC complexes include three major classes: cohesin, condensin and SMC5/6. However, the localization pattern and genetic requirements for the SMC5/6 complex during mammalian oogenesis have not previously been examined. In mouse oocytes, the SMC5/6 complex is enriched at the pericentromeric heterochromatin, and also localizes along chromosome arms during meiosis. The infertility phenotypes of females with a Zp3-Cre-driven conditional knockout (cKO) of Smc5 demonstrated that maternally expressed SMC5 protein is essential for early embryogenesis. Interestingly, protein levels of SMC5/6 complex components in oocytes decline as wild-type females age. When SMC5/6 complexes were completely absent in oocytes during meiotic resumption, homologous chromosomes failed to segregate accurately during meiosis I. Despite what appears to be an inability to resolve concatenation between chromosomes during meiosis, localization of topoisomerase IIα to bivalents was not affected; however, localization of condensin along the chromosome axes was perturbed. Taken together, these data demonstrate that the SMC5/6 complex is essential for the formation of segregation-competent bivalents during meiosis I, and findings suggest that age-dependent depletion of the SMC5/6 complex in oocytes could contribute to increased incidence of oocyte aneuploidy and spontaneous abortion in aging females.

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Control of meiotic pairing and recombination by chromosomally tethered 26 S proteasome

Cited by 82 publications

References 42 publications

The F-Box Protein ZYGO1 Mediates Bouquet Formation to Promote Homologous Pairing, Synapsis, and Recombination in Rice Meiosis

The F-Box Protein ZYGO1 Mediates Bouquet Formation to Promote Homologous Pairing, Synapsis, and Recombination in Rice Meiosis

A Surveillance System Ensures Crossover Formation in C. elegans

SMC5/6 is required for the formation of segregation-competent bivalent chromosomes during meiosis I in mouse oocytes

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