The Modulative Effects of Microcurrent Electrical Nerve Stimulation on Diabetic Mice

Abstract

Diabetes (one of non-communicable diseases) is serious due to its complications, such like, cardiovascular ailments, neuropathy, nephropathy, retinopathy, wound gangrene and sexual impotence. Diabetes and associated chronic conditions are rapidly emerging as major health problems. In clinical, there were different drugs for diabetes treatment on different mechanisms. However, there were limited studies on the efficacy of electric stimulations on diabetes therapeutic application. In current study, we try to evaluate the effect of microcurrent electrical nerve stimulator (MENS) on diabetes modulation as an alternative medicine. A total of 36 male ICR mice of 6 weeks old were randomly divided into 4 groups [1] Control, [2] MENS only, [3] DM, [4] DM with MENS. During 8 weeks treatments, the diabetes-associated assessments included body weight, diet utilization, blood glucose measurement, other biochemistries and histopathological observations. The diabetes animal model induced by STZ had 180 mg/dl fasting blood glucose (GLU-AC) before MENS intervention. After 3 and 6 weeks administration, the GLU-AC of DM+MENS group significantly decreased 31.97% and 50.82% (P< 0.0001), respectively, as compared to DM group and the OGTT also demonstrated the similar significant results. The diabetic syndromes of polydipsia and polyphagia were also significantly ameliorated by MENS intervention. In other biochemical indexes, the glycated hemoglobin (HbA1c), hyperinsulinemia, liver functions (AST& ALT) and kidneys function (BUN & Creatinine) were also significantly mitigated by MENS under diabetes model. The histological observation also showed the MENS administration improved the diabetes-related pathological characteristics in liver, kidney and pancreas tissues. Our results suggest that administration of MENS could significantly improve diabetes animal model on blood sugar homeostasis, diabetic polydipsia, biochemistries, and tissue damage. In the health conditions, the MENS didn’t exist obvious side effects on assessments. Therefore, the MENS could be potential on alternative medicine or supportive applications to future DM therapeutics.

A randomized trial on the effect of transcutaneous electrical nerve stimulator on glycemic control in patients with type 2 diabetes

“…On the contrary, our study device exhibited good safety profile and is well tolerated by most patients. Furthermore, GV has been linked to several acute and chronic micro- and macro-vascular complications related to diabetes 10 , 22 – 24 . Whether the beneficial effects of TENS on MAGE reduction can be translated to amelioration of short and long-term diabetic complications as well as improvement in quality of life in patients with type 2 diabetes needs to be investigated in the future.…”

“…Recently, a study in STZ-induced diabetes mellitus in mouse models showed that microcurrent electrical nerve stimulation exhibited effects similar to anti-diabetic drugs, through the peroxisome proliferator-activated receptor (PPAR), bile secretion, and insulin signaling pathways 9 . In a pre-clinical setting, we have proven that our TENS device significantly improved glucose parameters in a diabetic mouse model 10 , as indicated by fasting plasma glucose (FPG) and glycosylated hemoglobin (HbA 1c ) ( Appendix in Supplementary Material). Besides, TENS intervention reversed the pathological changes in liver, kidney, and pancreas in mice with diabetes mellitus induced by streptozotocin.…”

A Randomized Trial on the Effect of Transcutaneous Electrical Nerve Stimulator on Glycemic Control in Patients with Type 2 Diabetes

“…On the contrary, our study device exhibited good safety pro le and is well tolerated by most patients. Furthermore, GV has been linked to several acute and chronic micro-and macro-vascular complications related to diabetes 14,[25][26][27] . Whether the bene cial effects of TENS on MAGE reduction can be translated to amelioration of short and long-term diabetic complications as well as improvement in quality of life in patients with type 2 diabetes needs to be investigated in the future.…”

“…Recently, a study in STZinduced diabetes mellitus in mouse models showed that microcurrent electrical nerve stimulation exhibited effects similar to anti-diabetic drugs, through the peroxisome proliferator-activated receptor (PPAR), bile secretion, and insulin signaling pathways 13 . In a pre-clinical setting, we have proven that our TENS device signi cantly improved glucose parameters in a diabetic mouse model 14 , as indicated by fasting plasma glucose (FPG) and glycosylated hemoglobin (HbA 1c ). After a 6-week treatment period, FPG declined signi cantly in diabetic mice receiving TENS.…”

Combination of Treadmill Aerobic Exercise with <b><i>Bifidobacterium longum</i></b> OLP-01 Supplementation for Treatment of High-Fat Diet-Induced Obese Murine Model

“…Mice were fasted for 12 h and then orally administered 1.0 g of glucose per kg of body weight. The blood glucose from the blood samples (0.6 μL) collected at the indicated time points was measured using a glucometer (Accu-Chek ® ; Roche, Taipei, Taiwan) to determine plasma glucose concentration [22].…”