The Effects of Petroselinum Crispum on Estrogen Receptor-positive Benign and Malignant Mammary Cells (MCF12A/MCF7)

Schröder, Lennard; Koch, Julian; Mahner, Sven; Kost, Bernd; Hofmann, Simone; Jeschke, Udo; Haumann, Jens; Schmedt, Julian; Richter, Dagmar

doi:10.21873/anticanres.11294

Cited by 10 publications

(7 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Other authors stated that it weakly expressed ER [ 109 ] or was non-responsive to estrogen [ 92 ]. Contrarily, some authors affirmed that MCF12A is ER/PR positive [ 40 , 110 ] and that it even highly expresses ERα and ERβ [ 89 ]. SKBR3 presented overexpression of HER-2 as it was supposed [ 38 ], with more than 80% of the cells in the MCAs showing a thick, circumferential uniform membrane staining [ 111 ].…”

Section: Discussionmentioning

confidence: 99%

Morphometrical, Morphological, and Immunocytochemical Characterization of a Tool for Cytotoxicity Research: 3D Cultures of Breast Cell Lines Grown in Ultra-Low Attachment Plates

Malhão

Macedo

Ramos

et al. 2022

Toxics

View full text Add to dashboard Cite

Three-dimensional cell cultures may better mimic avascular tumors. Yet, they still lack characterization and standardization. Therefore, this study aimed to (a) generate multicellular aggregates (MCAs) of four breast cell lines: MCF7, MDA-MB-231, and SKBR3 (tumoral) and MCF12A (non-tumoral) using ultra-low attachment (ULA) plates, (b) detail the methodology used for their formation and analysis, providing technical tips, and (c) characterize the MCAs using morphometry, qualitative cytology (at light and electron microscopy), and quantitative immunocytochemistry (ICC) analysis. Each cell line generated uniform MCAs with structural differences among cell lines: MCF7 and MDA-MB-231 MCAs showed an ellipsoid/discoid shape and compact structure, while MCF12A and SKBR3 MCAs were loose, more flattened, and presented bigger areas. MCF7 MCAs revealed glandular breast differentiation features. ICC showed a random distribution of the proliferating and apoptotic cells throughout the MCAs, not fitting in the traditional spheroid model. ICC for cytokeratin, vimentin, and E-cadherin showed different results according to the cell lines. Estrogen (ER) and progesterone (PR) receptors were positive only in MCF7 and human epidermal growth factor receptor 2 (HER-2) in SKBR3. The presented characterization of the MCAs in non-exposed conditions provided a good baseline to evaluate the cytotoxic effects of potential anticancer compounds.

show abstract

Section: Discussionmentioning

confidence: 99%

Morphometrical, Morphological, and Immunocytochemical Characterization of a Tool for Cytotoxicity Research: 3D Cultures of Breast Cell Lines Grown in Ultra-Low Attachment Plates

Malhão

Macedo

Ramos

et al. 2022

Toxics

View full text Add to dashboard Cite

show abstract

“…The literature demonstrates that breast cancer cells can also be sensitive to parsley extract. For example, the root extract at a concentration of 500 μg/mL inhibited the DNA synthesis and metabolic activity of MCF712A and MCF7 estrogen receptor-positive benign and malignant mammary cells [ 48 ]. Tang et al have shown that dichloromethane extracts obtained from leaves and stems inhibit proliferation and migration of MCF7 cells [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Botanical Therapeutics: Phytochemical Screening and Biological Assessment of Chamomile, Parsley and Celery Extracts against A375 Human Melanoma and Dendritic Cells

Danciu

Zupkó

Bor

et al. 2018

IJMS

View full text Add to dashboard Cite

Chamomile, parsley, and celery represent major botanical sources of apigenin, a well-known flavone with chemopreventive properties. The aim of this study was to assess the phytochemical composition, antioxidant, and anti-inflammatory potential of methanol extracts obtained from chamomile, parsley, and celery collected from Romania, as well as the biological activity against A375 human melanoma and human dendritic cells. Results have shown that all three extracts are rich in polyphenolic compounds and flavonoids, and they generate a radical scavenger capacity, iron chelation potential, as well as lipoxygenase inhibition capacity. Chamomile and celery extracts present weak antiproliferative and pro-apoptotic properties in the set experimental conditions, while parsley extract draws out significant pro-apoptotic potential against A375 human melanoma cells. Parsley and chamomile extracts affected the fibroblast-like morphology of the screened tumor cell line. On the other hand, chamomile and celery extracts abrogated the expansion of LPS-activated dendritic cells, while the metabolic activity was attenuated by stimulation with celery extract; chamomile and parsley extracts had no effect upon this parameter. Chamomile and parsley extracts incubation with naive dendritic cells did not trigger cytokine secretion (TNF-alpha, IL-6, IL-10), but celery extract stimulation significantly reduced the anti-inflammatory, cytokine IL-10.

show abstract

“…It has been well established that MCF7 is an ERα-positive breast cancer cell line (23). In the current study, MCF7 were treated with E2 (1 mM) for 3 h, followed by analyses of cell viability and migration.…”

Section: E2 Treatment Promotes Mcf7 Cell Viability and Migrationmentioning

confidence: 94%

miR-148a Suppresses estrogen-induced viability and migration of breast cancer cells via inhibition of estrogen receptor α expression

Feng

et al. 2017

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

Abstract. MicroRNAs (miRs) play critical roles in the development and malignant progression of human cancers. miR-148a has previously been found to inhibit the migration and invasion of breast cancer cells. However, the underlying mechanism of miR-148a in regulating the viability and migration of estrogen receptor (ER) α-positive breast cancer cells is still unknown. In this study, ERα-positive breast cancer MCF7 cells were treated with estradiol (E2). Data from MTT and wound healing assays showed that E2 treatment promoted the viability and migration of MCF7 cells.

show abstract

The Effects of Petroselinum Crispum on Estrogen Receptor-positive Benign and Malignant Mammary Cells (MCF12A/MCF7)

Abstract: PCE showed antiproliferative effects in MCF7 and MCF12A cells. Further investigation is required to determine whether this effect can be solely attributed to its phytoestrogens.

Cited by 10 publications

References 24 publications

Morphometrical, Morphological, and Immunocytochemical Characterization of a Tool for Cytotoxicity Research: 3D Cultures of Breast Cell Lines Grown in Ultra-Low Attachment Plates

Morphometrical, Morphological, and Immunocytochemical Characterization of a Tool for Cytotoxicity Research: 3D Cultures of Breast Cell Lines Grown in Ultra-Low Attachment Plates

Botanical Therapeutics: Phytochemical Screening and Biological Assessment of Chamomile, Parsley and Celery Extracts against A375 Human Melanoma and Dendritic Cells

miR-148a Suppresses estrogen-induced viability and migration of breast cancer cells via inhibition of estrogen receptor α expression

Contact Info

Product

Resources

About