Transcriptional abundance of antioxidant enzymes in endometrium and their circulating levels in Zebu cows with and without uterine infection

Baithalu, Rubina Kumari; Singh, Sanjay K.; Kumaresan, A.; Mohanty, Ashok Kumar; Mohanty, T. K.; Kumar, Sunil; Kerketta, Shilpi; Maharana, Biswa Ranjan; Patbandha, T.K.; Attupuram, N.M.; Agarwal, Sudhir K.

doi:10.1016/j.anireprosci.2016.12.008

Cited by 14 publications

(7 citation statements)

References 39 publications

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“…Also, the obtained results TAC and GSH concentration recorded significant decrease in (G1) compared with (G2) while 8-OHdG and MDA enzyme showed significant increase. This results agreed with Inas et al (2019) and Baithalu et al (2017) who explain the decrease in total antioxidant to the over consumption of the total antioxidant during the protection against the invading organism. Bacterial infection sufficient to overwhelm the antioxidant defense uterus and cause cell damage, resulting in reductions in serum TCA and Gpx activities and an increase in MDA concentration.…”

Section: Discussionsupporting

confidence: 93%

Some Bacteriological and Immunological Studies of Endometritis in Mares Before and After Alternative Treatments Using Platelets Rich Plasma (And\or) Antibiotics

KORTAM

AREF

SAED

2022

Assiut Veterinary Medical Journal

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Bacterial endometritis is the most major cause of mare infertility. This study was carried out to investigate the efficiency of using alternative ways of treatment for endometritis by using selected antibiotics or platelet-rich plasma (PRP) or combinations between them. From a total of 46 mares, 39 suffered from endometritis (G1) and 7 were fertile mares (G2). The most commonly isolated bacterial species was E. coli (12 isolates, 30.77%) S. aureus (9 isolates, 23.07%), K. pneumoniae (8 isolates, 20.51%) followed by Strept. equi (7 isolates, 17.95) as single infection and Strept.equi+ E.coli+ K. pneumoniathen (3 isolates, 7.7%) as mixed infection .All isolates were susceptible to the widely used Amikacin and Gentamicin. Before the beginning of treatment, (G1) was subdivided into three groups: 13 mares treated with selected antibiotic (G3), 13 mares treated with PRP (G4), and 13 mares treated with a combination of antibiotics and PRP (G5). Our study recorded a significant increase in nitric oxide (NO), haptoglubine (HB), and malondialdehyde ( MDA) levels in (G1) compared with (G2), with significant decrease in total antioxidant (TAC). We found that the most effective result was detected in G5, which recorded marked decrease in bacteria isolates, significant decrease in NO, HB, polymorphnuclear neutrophils (PMN) and MDA levels. In addition to increase conception rate (92.3%) compared with other groups ,we concluded that using selected antibiotics with PRP in the treatment of endometritis was effective in decreasing uterine infection and have a great role in improving immunological status and elevate conception rate.

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Section: Discussionsupporting

confidence: 93%

Some Bacteriological and Immunological Studies of Endometritis in Mares Before and After Alternative Treatments Using Platelets Rich Plasma (And\or) Antibiotics

KORTAM

AREF

SAED

2022

Assiut Veterinary Medical Journal

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“…In vivo knockout studies have shown that mice lacking the entire GPX4 gene experience early embryonic lethality (Imai et al 2003) and that GPX4-deficient male mice are infertile (Schneider et al 2009). Although the presence of GPX4 has been shown in uteri from cows (Ramos et al 2015, Baithalu et al 2017 and pigs (Dalto et al 2015), the localization and physiological role of GPX4 has not been demonstrated in human and rodent reproductive tissues, including the uterus. Here, we show that GPX4 is widely expressed in the nonpregnant and pregnant rat uteri, including decidualized stromal cells.…”

Section: Figurementioning

confidence: 99%

Hyperandrogenism and insulin resistance modulate gravid uterine and placental ferroptosis in PCOS-like rats

Zhang

Jia

et al. 2020

Journal of Endocrinology

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Previous studies in rats showed that maternal exposure to 5α-dihydrotestosterone (DHT) and insulin (INS) from gestational day 7.5 to 13.5 induces hyperandrogenism and insulin resistance (HAIR) and subsequently leads to placental insufficiency and fetal loss. We therefore hypothesized that maternal HAIR triggers ferroptosis in the uterus and placenta in association with fetal loss in pregnant rats. Compared with controls, we found that co-exposure to DHT and INS led to decreased levels of Gpx4 and glutathione (GSH), increased GSH+glutathione disulfide (GSSG) and malondialdehyde (MDA), aberrant expression of ferroptosis-associated genes (Acsl4, Tfrc, Slc7a11, and Gclc), increased iron deposition, and activated ERK/p38/JNK phosphorylation in the gravid uterus. However, in the placenta, DHT and INS exposure only partially altered the expression of ferroptosis-related markers (e.g., Gpx4, GSH+GSSG, MDA, Gls2 and Slc7a11 mRNAs, and phosphorylated p38 levels). In the uteri co-exposed to DHT and INS, we also observed shrunken mitochondria with electron-dense cristae, and increased Dpp4 mRNA expression. In contrast, in placentas co-exposed to DHT and INS we found decreased Dpp4 mRNA expression and increased Cisd1 mRNA expression. Further, DHT+INS-exposed pregnant rats exhibited decreased apoptosis in the uterus and increased necroptosis in the placenta. Our findings suggest that maternal HAIR causes the activation of ferroptosis in the gravid uterus and placenta, although this is mediated via different mechanisms operating at the molecular and cellular levels. Furthermore, our data suggest other cell death pathways may play a role in coordinating or compensating for HAIR-induced ferroptosis when the gravid uterus and placenta are dysfunctional.

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“…In vivo knockout studies have shown that mice lacking the entire Gpx4 gene are early embryonic lethal (55), and Gpx4-deficient male mice are completely infertile (56). Although the presence of Gpx4 has been shown in uteri from cows (57, 58) and pigs (59), the physiological role and localization of Gpx4 have not been demonstrated in human and rodent reproductive tissues, including the uterus. Here, we show that Gpx4 is widely expressed in the human and rat uterus, including by decidualized stromal cells.…”

Section: Discussionmentioning

confidence: 99%

Induction of hyperandrogenism and insulin resistance differentially modulates ferroptosis in uterine and placental tissues of pregnant rats

Zhang

Jia

et al. 2020

Preprint

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Ferroptosis, a form of regulated necrotic cell death, plays roles in diverse physiological processes and diseases. Women with polycystic ovary syndrome (PCOS) have hyperandrogenism and insulin resistance (HAIR) and an increased risk of miscarriage and placental dysfunction during pregnancy. However, whether maternal HAIR alters mechanisms leading to ferroptosis in the gravid uterus and placenta remains unknown. Previous studies in rats showed that maternal exposure to 5α-dihydrotestosterone (DHT) and insulin (INS) from gestational day 7.5 to 13.5 induces HAIR and subsequently leads to placental insufficiency and fetal loss. We therefore hypothesized that maternal HAIR triggers ferroptosis in the uterus and placenta in association with fetal loss in pregnant rats. Compared with controls, we found that co-exposure to DHT and INS led to decreased levels of Gpx4 and glutathione (GSH), increased GSH+glutathione disulfide (GSSG) and malondialdehyde (MDA), aberrant expression of ferroptosis-associated genes (Acsl4, Tfrc, Slc7a11, and Gclc), increased iron deposition, and activated ERK/p38/JNK phosphorylation in the gravid uterus. However, in the placenta, DHT and INS exposure only partially altered the expression of ferroptosis-related markers (e.g., region-dependent Gpx4, GSH+GSSG, MDA, Gls2 and Slc7a11 mRNAs, and phosphorylated p38 levels). In the uteri co-exposed to DHT and INS, we also observed shrunken mitochondria with electron-dense cristae, which are key features of ferroptosis-related mitochondrial morphology, as well as increased expression of Dpp4, a mitochondria-encoded gene responsible for ferroptosis induction. In contrast, in placentas co-exposed to DHT and INS we found decreased expression of Dpp4 mRNA and increased expression of Cisd1 mRNA (a mitochondria-encoded iron-export factor). Further, DHT+INS-exposed pregnant rats exhibited decreased apoptosis in the uterus and increased necroptosis in the placenta. Our findings suggest that maternal HAIR causes the activation of ferroptosis in the gravid uterus and placenta, although this is mediated via different mechanisms operating at the molecular and cellular levels. Furthermore, our data suggest other cell death pathways may play a role in coordinating or compensating for HAIR-induced ferroptosis when the gravid uterus and placenta are dysfunctional.

show abstract

Transcriptional abundance of antioxidant enzymes in endometrium and their circulating levels in Zebu cows with and without uterine infection

Cited by 14 publications

References 39 publications

Some Bacteriological and Immunological Studies of Endometritis in Mares Before and After Alternative Treatments Using Platelets Rich Plasma (And\or) Antibiotics

Some Bacteriological and Immunological Studies of Endometritis in Mares Before and After Alternative Treatments Using Platelets Rich Plasma (And\or) Antibiotics

Hyperandrogenism and insulin resistance modulate gravid uterine and placental ferroptosis in PCOS-like rats

Induction of hyperandrogenism and insulin resistance differentially modulates ferroptosis in uterine and placental tissues of pregnant rats

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