Proteome analysis of human serum proteins adsorbed onto different titanium surfaces used in dental implants

Romero‐Gavilán, Francisco; Araújo‐Gomes, Nuno; Ródenas, Joaquin; Sánchez, Ana Borrego; Azkargorta, Mikel; Iloro, Ibón; Elortza, Félix; García‐Arnáez, Iñaki; Gurruchaga, M.; Goñi, Isabel; Suay, J.

doi:10.1080/08927014.2016.1259414

Cited by 48 publications

(46 citation statements)

References 104 publications

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“…Each of these slices was digested with trypsin and loaded onto a nanoACQUITY UPLC system connected online to a SYNAPT G2‐Si MS System (Waters, Milford, MA, USA). Differential protein analysis was carried out using Progenesis software (Nonlinear Dynamics, Newcastle, UK) as described before (Romero‐Gavilán et al, ), and the functional annotation of the proteins was performed using PANTHER (http://www.pantherdb.org/) and DAVID Go annotation programs (https://david.ncifcrf.gov/). Uniprot (http://www.uniprot.org/) nomenclature was adopted to name the proteins without the ending “_HUMAN”.…”

Section: Methodsmentioning

confidence: 99%

Silica-gelatin hybrid sol-gel coatings: A proteomic study with biocompatibility implications

Araújo‐Gomes

Romero‐Gavilán

Lara-Sáez

et al. 2018

J Tissue Eng Regen Med

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Osseointegration, including the foreign body reaction to biomaterials, is an immune-modulated, multifactorial, and complex healing process in which various cells and mediators are involved. The buildup of the osseointegration process is immunological and inflammation-driven, often triggered by the adsorption of proteins on the surfaces of the biomaterials and complement activation. New strategies for improving osseointegration use coatings as vehicles for osteogenic biomolecules delivery from implants. Natural polymers, such as gelatin, can mimic Collagen I and enhance the biocompatibility of a material. In this experimental study, two different base sol-gel formulations and their combination with gelatin were applied as coatings on sandblasted, acid-etched titanium substrates, and their biological potential as osteogenic biomaterials was tested. We examined the proteins adsorbed onto each surface and their in vitro and in vivo effects. In vitro results showed an improvement in cell proliferation and mineralization in gelatin-containing samples. In vivo testing showed the presence of a looser connective tissue layer in those coatings with substantially more complement activation proteins adsorbed, especially those containing gelatin. Vitronectin and FETUA, proteins associated with mineralization process, were significantly more adsorbed in gelatin coatings.

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Section: Methodsmentioning

confidence: 99%

Silica-gelatin hybrid sol-gel coatings: A proteomic study with biocompatibility implications

Araújo‐Gomes

Romero‐Gavilán

Lara-Sáez

et al. 2018

J Tissue Eng Regen Med

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“…From 218 identified human serum proteins, 30 were detected to be associated with bone metabolism. Of these, Apo E, antithrombin and protein C are found predominantly on sand-blasted or acid-etched Ti, whereas proteins of the complement system (C3) prefer adsorption onto smooth Ti surfaces [ 55 ].…”

Section: Titanium Surface Properties and Osteointegrationmentioning

confidence: 99%

Antimicrobial and Osseointegration Properties of Nanostructured Titanium Orthopaedic Implants

et al. 2017

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The surface design of titanium implants influences not only the local biological reactions but also affects at least the clinical result in orthopaedic application. During the last decades, strong efforts have been made to improve osteointegration and prevent bacterial adhesion to these surfaces. Following the rule of “smaller, faster, cheaper”, nanotechnology has encountered clinical application. It is evident that the hierarchical implant surface micro- and nanotopography orchestrate the biological cascades of early peri-implant endosseous healing or implant loosening. This review of the literature gives a brief overview of nanostructured titanium-base biomaterials designed to improve osteointegration and prevent from bacterial infection.

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“…Hong et al have studied the Ti properties in terms of its thrombogenic potential; it is one of the metals with high biocompatibility even though it lacks the bioactive properties . However, in previous studies of our group using mass spectrometry (LC/MS/MS), we have shown that two different Ti surface treatments, with slightly different in vivo behavior, display variations in the adsorbed first protein layer . These studies open up the exciting possibility of predicting the body reaction after implantation.…”

Section: Introductionmentioning

confidence: 99%

Characterization of serum proteins attached to distinct sol–gel hybrid surfaces

et al. 2017

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The success of a dental implant depends on its osseointegration, an important feature of the implant biocompatibility. In this study, two distinct sol-gel hybrid coating formulations [50% methyltrimethoxysilane: 50% 3-glycidoxypropyl-trimethoxysilane (50M50G) and 70% methyltrimethoxysilane with 30% tetraethyl orthosilicate (70M30T)] were applied onto titanium implants. To evaluate their osseointegration, in vitro and in vivo assays were performed. Cell proliferation and differentiation in vitro did not show any differences between the coatings. However, four and eight weeks after in vivo implantation, the fibrous capsule area surrounding 50M50G-implant was 10 and 4 times, respectively, bigger than the area of connective tissue surrounding the 70M30T treated implant. Thus, the in vitro results gave no prediction or explanation for the 50M50G-implant failure in vivo. We hypothesized that the first protein layer adhered to the surface may have direct implication in implant osseointegration, and perhaps correlate with the in vivo outcome. Human serum was used for adsorption analysis on the biomaterials, the first layer of serum proteins adhered to the implant surface was analyzed by proteomic analysis, using mass spectrometry (LC-MS/MS). From the 171 proteins identified; 30 proteins were significantly enriched on the 50M50G implant surface. This group comprised numerous proteins of the immune complement system, including several subcomponents of the C1 complement, complement factor H, C4b-binding protein alpha chain, complement C5 and C-reactive protein. This result suggests that these proteins enriched in 50M50G surface might trigger the cascade leading to the formation of the fibrous capsule observed. The implications of these results could open up future possibilities to predict the biocompatibility problems in vivo. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1477-1485, 2018.

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Proteome analysis of human serum proteins adsorbed onto different titanium surfaces used in dental implants

Cited by 48 publications

References 104 publications

Silica-gelatin hybrid sol-gel coatings: A proteomic study with biocompatibility implications

Silica-gelatin hybrid sol-gel coatings: A proteomic study with biocompatibility implications

Antimicrobial and Osseointegration Properties of Nanostructured Titanium Orthopaedic Implants

Characterization of serum proteins attached to distinct sol–gel hybrid surfaces

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