A novel tomato SUMO E3 ligase, SlSIZ1, confers drought tolerance in transgenic tobacco

Zhang, Song; Zhuang, Kunyang; Wang, Shiju; Lv, Jinlian; Ma, Nana; Meng, Qingwei

doi:10.1111/jipb.12514

Cited by 52 publications

(21 citation statements)

References 47 publications

(86 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Conversely, heat‐induced sumoylation of target proteins was observed in several previous studies . In addition, rice E3 SUMO ligases OsSIZ1 and OsSIZ2 were involved in sumoylation of target proteins under heat stress , and transgenic Arabidopsis overexpressing tomato E3 SUMO ligase SISIZ1 also accumulated SUMO conjugates in response to heat stress . There are three types of E3 SUMO ligase in yeast and animal systems: SIZ/PIAS, RanBP2, and polycomb .…”

Section: Discussionmentioning

confidence: 77%

Post‐translational modifications of Arabidopsis E3 SUMO ligase AtSIZ1 are controlled by environmental conditions

2017

View full text Add to dashboard Cite

Sumoylation regulates numerous cellular functions in plants as well as in other eukaryotic systems. However, the regulatory mechanisms controlling E3 small ubiquitin‐related modifier (SUMO) ligase are not well understood. Here, post‐translational modification of the Arabidopsis E3 SUMO ligase AtSIZ1 was shown to be specifically controlled by abiotic stresses. AtSIZ1 ubiquitination was induced by exposure to heat stress in transgenic plants overexpressing the E3 ubiquitin ligase COP1. In addition, AtSIZ1 ubiquitination was strongly enhanced in transgenic plants overexpressing SUMO isopeptidase ESD4 under heat stress. By contrast, drought stress induced sumoylation rather than ubiquitination of AtSIZ1 and sumoylated forms of AtSIZ1 accumulated in esd4 and cop1–4 mutants. Moreover, siz1 mutants were found to be tolerant to heat and drought stresses. Taken together, these results indicate that ubiquitination and sumoylation of AtSIZ1 in response to abiotic stresses depend on the activities of COP1 and ESD4 and that the activity and stability of AtSIZ1 can be specifically controlled by different abiotic stresses.

show abstract

Section: Discussionmentioning

confidence: 77%

Post‐translational modifications of Arabidopsis E3 SUMO ligase AtSIZ1 are controlled by environmental conditions

2017

View full text Add to dashboard Cite

show abstract

“…Tobacco seeds were sterilized with 1% sodium hypochlorite for 15 min, washed three times using sterile water, and then sown on 1/2 MS medium. Seven-day-old seedlings were transferred into nutrient soil in a greenhouse at 25/22 • C (day/night) with a 16-h photoperiod and 50-60% relative humidity as described previously (Zhang et al, 2017). Tobacco plants with four leaves were infected.…”

Section: Transcriptional Activation Assay In Tobacco Plantsmentioning

confidence: 99%

GmNFYA13 Improves Salt and Drought Tolerance in Transgenic Soybean Plants

Tang

et al. 2020

Front. Plant Sci.

View full text Add to dashboard Cite

NF-YA transcription factors function in modulating tolerance to abiotic stresses that are serious threats to crop yields. In this study, GmNFYA13, an NF-YA gene in soybean, was strongly induced by salt, drought, ABA, and H 2 O 2 , and suppressed by tungstate, an ABA synthesis inhibitor. The GmNFYA13 transcripts were detected in different tissues in seedling and flowering stages, and the expression levels in roots were highest. GmNFYA13 is a nuclear localization protein with self-activating activity. Transgenic Arabidopsis plants overexpressing GmNFYA13 with higher transcript levels of stressrelated genes showed ABA hypersensitivity and enhanced tolerance to salt and drought stresses compared with WT plants. Moreover, overexpression of GmNFYA13 resulted in higher salt and drought tolerance in OE soybean plants, while suppressing it produced the opposite results. In addition, GmNFYA13 could bind to the promoters of GmSALT3, GmMYB84, GmNCED3, and GmRbohB to regulate their expression abundance in vivo. The data in this study suggested that GmNFYA13 enhanced salt and drought tolerance in soybean plants.

show abstract

“…umoylation, the covalent attachment of small ubiquitin-like modifier (SUMO) to other proteins, is a post-translational modification that controls protein function, activity, localization, and turnover in eukaryotes 1,2 . In plants, sumoylation is involved in the response to abiotic and biotic stresses, such as cold, salt, and drought stresses, and innate immunity [3][4][5][6][7][8] . Furthermore, sumoylation regulates signaling pathways for plant hormones, including abscisic acid (ABA) and salicylic acid 7,[9][10][11][12] .…”

mentioning

confidence: 99%

The PHD finger of Arabidopsis SIZ1 recognizes trimethylated histone H3K4 mediating SIZ1 function and abiotic stress response

2020

View full text Add to dashboard Cite

Arabidopsis SIZ1 encodes a SUMO E3 ligase to regulate abiotic and biotic stress responses. Among SIZ1 or mammalian PIAS orthologs, plant SIZ1 proteins contain the plant homeodomain (PHD) finger, a C 4 HC 3 zinc finger. Here, we investigated the importance of PHD of Arabidopsis SIZ1. The Pro SIZ1 ::SIZ1(ΔPHD):GFP was unable to complement growth retardation, ABA hypersensitivity, and the cold-sensitive phenotype of the siz1 mutant, but Pro SIZ1 ::SIZ1: GFP could. Substitution of C162S in the PHD finger was unable to complement the siz1 mutation. Tri-methylated histone H3K4 (H3K4me3) was recognized by PHD, not by PHD (C162S). WRKY70 was up-regulated in the siz1-2 mutant and H3K4me3 accumulated at high levels in the WRKY70 promoter. PHD interacts with ATX, which mediates methylation of histone, probably leading to suppression of ATX's function. These results suggest that the PHD finger of SIZ1 is important for recognition of the histone code and is required for SIZ1 function and transcriptional suppression.

show abstract

A novel tomato SUMO E3 ligase, SlSIZ1, confers drought tolerance in transgenic tobacco

Cited by 52 publications

References 47 publications

Post‐translational modifications of Arabidopsis E3 SUMO ligase AtSIZ1 are controlled by environmental conditions

Post‐translational modifications of Arabidopsis E3 SUMO ligase AtSIZ1 are controlled by environmental conditions

GmNFYA13 Improves Salt and Drought Tolerance in Transgenic Soybean Plants

The PHD finger of Arabidopsis SIZ1 recognizes trimethylated histone H3K4 mediating SIZ1 function and abiotic stress response

Contact Info

Product

Resources

About