In Vitro Modulation of TrkB Receptor Signaling upon Sequential Delivery of Curcumin-DHA Loaded Carriers Towards Promoting Neuronal Survival

Guerzoni, Luis P. B.; Nicolas, Valérie; Angelova, Angelina

doi:10.1007/s11095-016-2080-4

Cited by 71 publications

(37 citation statements)

References 97 publications

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“…It should be assumed that constant overexpression causes compensatory processes in neural cells and that transient effects are not commensurable with chronically increased concentration. The main functions of BDNF are mediated by its interaction with tropomyosin-related kinase B (TrkB) receptor, which could be also considered a potential therapeutic agent [55,56]. In this regard, investigations have demonstrated that increasing BDNF expression at the target sites leads to suppression of TrkB receptors, and thereby reduces the effect of BDNF chronic delivery over time.…”

Section: Discussionmentioning

confidence: 99%

AAV-Syn-BDNF-EGFP Virus Construct Exerts Neuroprotective Action on the Hippocampal Neural Network during Hypoxia In Vitro

Митрошина

Mishchenko

Usenko

et al. 2018

IJMS

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Brain-derived neurotrophic factor (BDNF) is one of the key signaling molecules that supports the viability of neural cells in various brain pathologies, and can be considered a potential therapeutic agent. However, several methodological difficulties, such as overcoming the blood–brain barrier and the short half-life period, challenge the potential use of BDNF in clinical practice. Gene therapy could overcome these limitations. Investigating the influence of viral vectors on the neural network level is of particular interest because viral overexpression affects different aspects of cell metabolism and interactions between neurons. The present work aimed to investigate the influence of the adeno-associated virus (AAV)-Syn-BDNF-EGFP virus construct on neural network activity parameters in an acute hypobaric hypoxia model in vitro. Materials and methods. An adeno-associated virus vector carrying the BDNF gene was constructed using the following plasmids: AAV-Syn-EGFP, pDP5, DJvector, and pHelper. The developed virus vector was then tested on primary hippocampal cultures obtained from C57BL/6 mouse embryos (E18). Acute hypobaric hypoxia was induced on day 21 in vitro. Spontaneous bioelectrical and calcium activity of neural networks in primary cultures and viability tests were analysed during normoxia and during the posthypoxic period. Results. BDNF overexpression by AAV-Syn-BDNF-EGFP does not affect cell viability or the main parameters of spontaneous bioelectrical activity in normoxia. Application of the developed virus construct partially eliminates the negative hypoxic consequences by preserving cell viability and maintaining spontaneous bioelectrical activity in the cultures. Moreover, the internal functional structure, including the activation pattern of network bursts, the number of hubs, and the number of connections within network elements, is also partially preserved. BDNF overexpression prevents a decrease in the number of cells exhibiting calcium activity and maintains the frequency of calcium oscillations. Conclusion. This study revealed the pronounced antihypoxic and neuroprotective effects of AAV-Syn-BDNF-EGFP virus transduction in an acute normobaric hypoxia model.

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Section: Discussionmentioning

confidence: 99%

AAV-Syn-BDNF-EGFP Virus Construct Exerts Neuroprotective Action on the Hippocampal Neural Network during Hypoxia In Vitro

Митрошина

Mishchenko

Usenko

et al. 2018

IJMS

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“…The phase transitions between lyotropic liquid crystalline states [1][2][3] of synthetic lipid membranes have broad applications in many fields, for instance in the crystallization of proteins and other bioactive compounds, the encapsulation of genetic material, the development of drug delivery systems and carriers for the protection of instable dyes, diagnostic agents, or food ingredients [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]. Significant progress in the understanding of the involved structural mechanisms can be achieved by the small-angle X-ray scattering (SAXS) method [21][22][23][24][25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

A vesicle-to-sponge transition via the proliferation of membrane-linking pores in ω-3 polyunsaturated fatty acid-containing lipid assemblies

Angelova

Angelov

Garamus

et al. 2019

Journal of Molecular Liquids

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 Mixed membrane assemblies of monoolein and a -3 polyunsaturated fatty acid characterized by non-trivial porous topologies  Amphiphilic composition-triggered transition from a vesicle to a sponge structure at room temperature  Small-angle X-ray scattering (SAXS) patterns detect the internal nanostructure transformation due to curvature changes  Cryo-TEM imaging reveals a sequence of intermediate states upon the vesicle-to-sponge nanoparticle transition

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“…[27][28][29][30][31][32][33][34][35][36][37][38] In addition, interests have also been generated to study the effects of nanoparticles on neurons and there are several reports that suggest that nanoparticles promote neuronal differentiation, and neuroprotection studied in both in vitro and in vivo conditions. 3,[39][40][41][42][43] To get better therapeutic results, various types of nanoparticles have been studied in neurons, and among those, carbon-based nanoparticles are mostly reported, 4,[44][45][46][47][48] followed by gold and silver nanoparticles (AgNPs). [49][50][51] Despite having many beneficial properties, nanoparticle also raises few health hazard and toxicity issues.…”

Section: Introduction Of Nanoparticlesmentioning

confidence: 99%

Impact of nanoparticles on neuron biology: current research trends

Khan

Almohazey

Alomari

et al. 2018

IJN

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Nanoparticles have enormous applications in textiles, cosmetics, electronics, and pharmaceuticals. But due to their exceptional physical and chemical properties, particularly antimicrobial, anticancer, antibacterial, anti-inflammatory properties, nanoparticles have many potential applications in diagnosis as well as in the treatment of various diseases. Over the past few years, nanoparticles have been extensively used to investigate their response on the neuronal cells. These nanoparticles cause stem cells to differentiate into neuronal cells and promote neuronal cell survivability and neuronal cell growth and expansion. The nanoparticles have been tested both in in vitro and in vivo models. The nanoparticles with various shapes, sizes, and chemical compositions mostly produced stimulatory effects on neuronal cells, but there are few that can cause inhibitory effects on the neuronal cells. In this review, we discuss stimulatory and inhibitory effects of various nanoparticles on the neuronal cells. The aim of this review was to summarize different effects of nanoparticles on the neuronal cells and try to understand the differential response of various nanoparticles. This review provides a bird’s eye view approach on the effects of various nanoparticles on neuronal differentiation, neuronal survivability, neuronal growth, neuronal cell adhesion, and functional and behavioral recovery. Finally, this review helps the researchers to understand the different roles of nanoparticles (stimulatory and inhibitory) in neuronal cells to develop effective therapeutic and diagnostic strategies for neurodegenerative diseases.

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In Vitro Modulation of TrkB Receptor Signaling upon Sequential Delivery of Curcumin-DHA Loaded Carriers Towards Promoting Neuronal Survival

Cited by 71 publications

References 97 publications

AAV-Syn-BDNF-EGFP Virus Construct Exerts Neuroprotective Action on the Hippocampal Neural Network during Hypoxia In Vitro

AAV-Syn-BDNF-EGFP Virus Construct Exerts Neuroprotective Action on the Hippocampal Neural Network during Hypoxia In Vitro

A vesicle-to-sponge transition via the proliferation of membrane-linking pores in ω-3 polyunsaturated fatty acid-containing lipid assemblies

Impact of nanoparticles on neuron biology: current research trends

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