Hesa-A Effects on Cell Cycle Signaling in Esophageal Carcinoma Cell Line

Ahmadian, Nasser; Pashaei-Asl, Roghiyeh; Samadi, Nasser; Rahmati-Yamchi, Mohammad; Rashidi, Mohammad‐Reza; Ahmadian, Masomeh; Esmaeili, Moosa; Salamat, Fatemeh; Besharat, Sima; Joshaghani, Hamidreza

doi:10.15171/mejdd.2016.39

Cited by 6 publications

(3 citation statements)

References 16 publications

(14 reference statements)

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“… 44 p21 is expressed by both p53-dependent and independent mechanisms after stress. 45 In cell cycle arrest pathway, p53 affects p21 expression, thus p21 stimulation inhibits tumor development and causes cell arrest; 45 , 46 however, it can be activated independently and can have cancer-promoting properties. 47 Therefore, the control of p53's transcriptional activity is critical for novel therapeutic approaches to design drugs for ovarian cancer treatment.…”

Section: Discussionmentioning

confidence: 99%

The Inhibitory Effect of Ginger Extract on Ovarian Cancer Cell Line; Application of Systems Biology

Pashaei-Asl

Gharabaghi

et al. 2017

Adv Pharm Bull

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Purpose: Ginger is a natural compound with anti-cancer properties. The effects of ginger and its mechanism on ovarian cancer and its cell line model, SKOV-3, are unclear. In this study, we have evaluated the effect of ginger extract on SKOV-3. Methods: SKOV-3 cells were incubated with ginger extract for 24, 48 and 72 hours. Cell toxicity assay was performed. Different data mining algorithms were applied to highlight the most important features contributing to ginger inhibition on the SKOV-3 cell proliferation. Moreover, Real-Time PCR was performed to assay p53, p21 and bcl-2 genes expression. For co-expression meta-analysis of p53, mutual ranking (MR) index and transformation to Z-values (Z distribution) were applied on available transcriptome data in NCBI GEO data repository. Results: The ginger extract significantly inhibited cancer growth in ovarian cancer cell line. The most important attribute was 60 µg/ml concentration which received weights higher than 0.50, 0.75 and 0.95 by 90%, 80% and 50% of feature selection models, respectively. The expression level of p53 was increased sharply in response to ginger treatment. Systems biology analysis and meta-analysis of deposited expression value in NCBI based on rank of correlation and Z-transformation approach unraveled the key co-expressed genes and co-expressed network of P53, as the key transcription factor induced by ginger extract. High co-expression between P53 and the other apoptosis-inducing proteins such as CASP2 and DEDD was noticeable, suggesting the molecular mechanism underpinning of ginger action. Conclusion: We found that the ginger extract has anticancer properties through p53 pathway to induce apoptosis.

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Section: Discussionmentioning

confidence: 99%

The Inhibitory Effect of Ginger Extract on Ovarian Cancer Cell Line; Application of Systems Biology

Pashaei-Asl

Gharabaghi

et al. 2017

Adv Pharm Bull

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“…To determine the effect of hAFMSCs-CM, cell viability was evaluated using MTT (3-(4, 5-dimethylthiazol-2- yl)-2, 5-diphenyltetrazolium bromide) (Sigma, Cas# 298-93-1, USA) assay, as explained elsewhere. 6 , 41 MCF-7 and Hu02 cells were treated with different percentages of hAFMSCs-CM (20%, 40%, & 80%) for 24, 48, and 72 hours, respectively. In order to determine the cell viability, 0.5 mg/mL of MTT reagent was added to each well and incubated for 4 hours.…”

Section: Methodsmentioning

confidence: 99%

Apoptotic effects of human amniotic fluid mesenchymal stem cells conditioned medium on human MCF-7 breast cancer cell line

et al. 2022

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Introduction: Breast cancer, as the most common malignancy among women, is shown to have a high mortality rate and resistance to chemotherapy. Research has shown the possible inhibitory role of Mesenchymal stem cells in curing cancer. Thus, the present work used human amniotic fluid mesenchymal stem cell-conditioned medium (hAFMSCs-CM) as an apoptotic reagent on the human MCF-7 breast cancer cell line. Methods: Conditioned medium (CM) was prepared from hAFMSCs. After treating MCF-7 cells with CM, a number of analytical procedures (MTT, real-time PCR, western blot, and flow cytometry) were recruited to evaluate the cell viability, Bax and Bcl-2 gene expression, P53 protein expression, and apoptosis, respectively. Human fibroblast cells (Hu02) were used as the negative control. In addition, an integrated approach to meta-analysis was performed. Results: The MCF-7 cells’ viability was decreased significantly after 24 hours (P < 0.0001) and 72 hours (P < 0.05) of treatment. Compared with the control cells, Bax gene’s mRNA expression increased and Bcl-2’s mRNA expression decreased considerably after treating for 24 hours with 80% hAFMSCs-CM (P = 0.0012, P < 0.0001, respectively); an increasing pattern in P53 protein expression could also be observed. The flow cytometry analysis indicated apoptosis. Results from literature mining and the integrated meta-analysis showed that hAFMSCs-CM is able to activate a molecular network where Bcl2 downregulation stands in harmony with the upregulation of P53, EIF5A, DDB2, and Bax, leading to the activation of apoptosis. Conclusion: Our finding demonstrated that hAFMSCs-CM presents apoptotic effect on MCF-7 cells; therefore, the application of hAFMSCs-CM, as a therapeutic reagent, can suppress breast cancer cells’ viabilities and induce apoptosis.

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“…The incidence and progression of EC are a multistep process accompanied with activation of oncogenes and inactivation of tumor suppressor genes (21,22). It has been demonstrated that EC progression is strongly linked with cell proliferation, survival, invasion, metastasis and angiogenesis, cell adhesion, the imbalance of oncogene and tumor suppressor gene expression and participation of the immune system.…”

Section: Molecular Mechanism Of Ecmentioning

confidence: 99%

Association of Selenium and Risk of Esophageal Cancer: A Review

Younesian

Hosseinzadeh

et al. 2020

mljgoums

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Esophageal cancer (EC) is one of the most common types of cancer, especially in Asia. Esophageal squamous cell cancer (ESCC) is the most important histological subtype of EC, which accounts for 90% of all EC cases worldwide. ESCC is highly prevalent in Turkey, Iran, Kazakhstan and northern and central parts of China. Selenium is an essential micronutrient that is required for cellular functioning and synthesis of several selenoproteins. It also modulates the antioxidant defense system, cell cycle and apoptosis. This article reviews the most important molecular mechanisms of EC and investigates the association between selenium level and incidence of EC in high-risk areas.

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Hesa-A Effects on Cell Cycle Signaling in Esophageal Carcinoma Cell Line

Cited by 6 publications

References 16 publications

The Inhibitory Effect of Ginger Extract on Ovarian Cancer Cell Line; Application of Systems Biology

The Inhibitory Effect of Ginger Extract on Ovarian Cancer Cell Line; Application of Systems Biology

Apoptotic effects of human amniotic fluid mesenchymal stem cells conditioned medium on human MCF-7 breast cancer cell line

Association of Selenium and Risk of Esophageal Cancer: A Review

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