Enhanced candicidal compound production by a new soil isolate Penicillium verruculosum MKH7 under submerged fermentation

Abstract

BackgroundMicroorganisms are a rich source of structurally diverse secondary metabolites that exert a major impact on the control of infectious diseases and other medical conditions. The biosynthesis of these metabolites can be improved by manipulating the nutritional or environmental factors. This work evaluates the effects of fermentation parameters on the production of a lactone compound effective against Candida albicans by Penicillium verruculosum MKH7 under submerged fermentation. Design–Expert version8.0 software was used for construction of the experimental design and statistical analysis of the experimental data.ResultsThe important factors influencing antibiotic production selected in accordance with the Plackett–Burman design were found to be initial pH, temperature, peptone, MgSO4.7H2O. Orthogonal central composite design and response surface methodology were adopted to further investigate the mutual interaction between the variables and identify the optimum values that catalyse maximum metabolite production. The determination coefficient (R2) of the fitted second order model was 0.9852. The validation experiments using optimized conditions of initial pH 7.4, temperature 27 °C, peptone 9.2 g/l and MgSO4.7H2O 0.39 g/l resulted in a significant increase (almost 7 fold from 30 to 205.5 mg/l) in the metabolite production which was in agreement with the prediction (211.24 mg/l). Stability of the compound was also assessed on the basis of its response to physical and chemical stresses.ConclusionsSo far as our knowledge goes, till date there are no reports available on the production of antibiotics by Penicillium verruculosum through media optimization using RSM. Optimization not only led to a 7 fold increase in metabolite yield but the same was achieved at much lesser time (8–10 days compared to the earlier 12–15 days). The enhanced yield of the antibiotic strongly suggests that the fungus P. verruculosum MKH7 can be efficiently used for antibiotic production on a large scale.

Optimization of fermentation conditions through response surface methodology for enhanced antibacterial metabolite production by Streptomyces sp. 1-14 from cassava rhizosphere

“…Temperature is a physiological parameter that alters the fermentation process. Fermentation can affect the regulation of ATP, which, in turn, influences the regulation of metabolic pathways and cell-wall synthesis, thereby causing diverse metabolic effects and generating a variety of products [ 51 – 52 ]. Due to the slow growth of actinomycetes, the biological activity of these organisms may be affected at high or low temperatures [ 52 ].…”

Simultaneous saccharification and fermentation of sequential dilute acid‐alkali pretreated cotton (Gossypium hirsutum L.) stalk for cellulosic ethanol production

“…The phylogenetic tree was constructed by neighborhood joining method using Molecular Evolutionary Genetics Analysis (MEGA) version-7 and boot-strap analysis based on 1000 replicates. 16 Sequential dilute acid-alkali pretreatment CS was milled in the Willey Mill and sieved through 40 mm mesh screen. The milled CS was kept airtight at room temperature for further use.…”

Prospecção enzimática e atividade antimicrobiana de espécies de Penicillium isoladas do Bioma Amazônico / Enzyme prospection and antimicrobial activity of Penicillium species isolated from the Amazon Biome

“…A produção de antimicrobianos por Penicillium é influenciada por diversos parâmetros como tipo de bioprocesso, temperatura e a espécie (BARREIRO; MARTÍN; GARCIA-ESTRADA, 2012;ZERROUG et al, 2018). De acordo com Talukdar et al (2016), a síntese de compostos bioativos por espécies de Penicillium com ação inibitória contra microrganismos também é influenciada pela composição do meio de cultivo, com destaque para as fontes de carbono, nitrogênio e minerais. Penicillium isoladas do bioma amazônico são fontes promissoras de biocompostos com potencial para uso nas indústrias de alimentos, medicamentos, de detergente e no beneficiamento de couro.…”