“…During the past decade, advances in DNA sequencing technology have allowed the identification of a wide range of novel viruses associated with mosquitoes (Aguiar et al, 2015; Auguste et al, 2014; Bolling et al, 2015; Carissimo et al, 2016; Chandler et al, 2015; Charles et al, 2016; Cholleti et al, 2016; Coffey et al, 2014; Contreras-Gutierrez et al, 2017; Fauver et al, 2016; Frey et al, 2016; Li et al, 2015a; Li et al, 2015b; Nasar et al, 2012; Ng et al, 2011; Palacios et al, 2015; Pauvolid-Correa et al, 2016; Shi et al, 2015; Simmonds et al, 2017; Vasilakis et al, 2014a; Wang et al, 2015). We examine here, by electron microscopy and metagenomics analysis, 11 mosquito derived viral isolates from the extensive collection of the World Reference Center for Emerging Viruses and Arboviruses (WRCEVA) (https://www.niaid.nih.gov/research/wreceva), whose morphology indicated the likely presence of multi-segmented reoviruses.…”