According to the Article “Cytokine Expression in Human Osteoblasts After Antiseptic Treatment: A Comparative Study between Polyhexanide and Chlorhexidine” by Röhner E. et al., Journal of Investigative Surgery, 28, 1–7, 2015

Eberlein, Thomas; Wiegand, Cornelia; Brill, Florian H. H.

doi:10.1080/08941939.2016.1254307

Cited by 1 publication

(1 citation statement)

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“…Other authors have described similar decreases in function alongside indicators of cell damage [22][23][24]. Some of these results have been challenged [25], whereas other investigators have found results to be insignificant vs control groups [26,27].…”

Section: Introductionmentioning

confidence: 97%

The Effect of Different Irrigation Solutions on the Cytotoxicity and Recovery Potential of Human Osteoblast Cells In Vitro

et al. 2021

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Background: Surgeons use various irrigation solutions to minimize the risk of prosthetic joint infection after total joint arthroplasty. The toxicity of these solutions is an important consideration in their use. This study investigates the effect of irrigation solutions Bacitracin, Clorpactin (sodium oxychlorosene), and Irrisept (chlorhexidine) on osteoblast cytotoxicity and proliferation. Methods: Four replicates of 6 conditions at 3 time points (1, 2, and 4 min) were tested: control (normal saline), Bacitracin (33 IU/ml), Clorpactin (0.05%, 0.1%, 0.2%), and Irrisept (0.05% chlorhexidine gluconate). Human osteoblasts were cultured at 37 C and 5% CO 2 until confluent monolayers were obtained. The treatment solution was applied, and cells were washed 3x with warm phosphate-buffered saline and then supplemented with a fresh medium. Phase-contrast images were taken before and after treatment. The cytotoxicity and proliferation of the treated cells was measured for all conditions on day 3 and day 5 after treatment using the alamarBlue assay. Results: All test conditions showed morphological changes to cells after treatment; controls did not. Cells demonstrated curling and detachment. This effect was the worst and permanent with Irrisept, whereas other treatments showed a return to normal morphology after 1 week. All treatments showed increased %alamarBlue reduction after 5 days except Irrisept, which showed decreased reduction. There was no statistically significant time or dose dependence with Clorpactin treatment. Conclusions: Clorpactin and Bacitracin are damaging to human osteoblast cells in vitro as compared with normal saline. This damage is at least partially reversible as shown by morphology and cell viability assay. Irrisept caused more damage than either Clorpactin or Bacitracin, and the damage was not reversible.

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