Soortia roseihalophila gen. nov., sp. nov., a new taxon in the order Balneolales isolated from a travertine spring, and description of Soortiaceae fam. nov.

Abstract

A novel Gram-stain-negative, slightly halophilic, motile, curved rod with a horseshoe shape, designated strain Bsw-2bT, was isolated from Badab-Soort travertine spring in Iran. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain Bsw-2bT belongs to the order Balneolales, showing 84.6 % sequence similarity to Gracilimonastropica DSM 19535T and 84.4 % and 83.9 % sequence similarity to Gracilimonas rosea CL-KR2T and Balneola vulgaris DSM 17893T, respectively. In addition, phenotypic and physiological features could clearly differentiate strain Bsw-2bT from species of the most closely related genera, Gracilimonas, Balneola, Aliifodinibius and Fodinibius. The strain was able to grow with 1-3 % (w/v) (optimum at 2 %) NaCl, at temperatures of 28-34 °C (optimum at 30 °C) and between pH 6.0 and 8.0 (optimum at pH 7.0). The major cellular fatty acids of strain Bsw-2bT were iso-C15 : 0, iso-C13 : 0 and iso-C14 : 0. The polar lipid profile of strain Bsw-2bT was composed predominantly of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, an unknown glycolipid and four unknown phospholipids. The DNA G+C content was 40.5 mol%. Based on the evidence from the polyphasic study, strain Bsw-2bT represents a novel species in a novel genus within a new family, for which the name Soortia roseihalophila gen. nov., sp. nov. is proposed, within the new family Soortiaceae fam. nov. The type strain is strain Bsw-2bT (=IBRC-M 10915T=LMG 28547T).

Natronogracilivirga saccharolytica gen. nov., sp. nov. and Cyclonatronum proteinivorum gen. nov., sp. nov., haloalkaliphilic organotrophic bacteroidetes from hypersaline soda lakes forming a new family Cyclonatronaceae fam. nov. in the order Balneolales

“…Table 1 Comparison of the novel genera Natronogracilivirga and Cyclonatronum with the type species of other genera of Balneolales. 1strain Z-1702 T (this work); 2strain Omega T (this work and (Sorokin et al, 2018); 3-Balneola vulgaris (Urios et al, 2006); 4 -Gracilimonas tropica (Cho et al, 2013;Choi et al, 2009); 5 -Fodinibius salinus (Wang et al, 2012); 6 -Aliifodinibius roseus (Wang et al, 2013); 7 -Rhodohalobacter halophilus (Xia et al, 2017;Han et al, 2018); 8 -Soortia roseihalophila (Amoozegar et al, 2017). Phenotypic properties of strain Z-1702 T…”

“…16S rRNA gene sequence-based phylogenetic analysis revealed that the Balneolales comprised of three clusters: i) located most closely to the root Soortia roseihalophila (Amoozegar et al, 2017), ii) the cluster consisting of strain Z-1702 T and stain Omega T (Sorokin et al, 2018) and iii) the cluster containing the rest of the Balneolales members. Considering that Soortia roseihalophila and all other Balneolales represented 2 different families: Soortiaceae and Balneolaceae, respectively, and taking into account the evolutionary distance of the bifurcation points of the "Z-1702 T -Omega T " cluster, it would be a solid assumption to consider the latter as a novel-family lineage.…”