Lacibacter nakdongensis sp. nov., isolated from river sediment

Abstract

A novel Gram-stain-negative, non-spore-forming, orange-pigmented bacterium, designated strain SS2-56T, was isolated from sediment of the Nakdong River in Sangju-si, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate SS2-56T belongs to the family Chitinophagaceae, and was most closely related to Lacibacter daechungensis H32-4T (96.6 % similarity) and Lacibacter cauensis NJ-8T (96.1 %). Strain SS2-56T contained menaquinone 7 (MK-7) as a respiratory quinone and iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 1 G, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0 as major fatty acids. The major polar lipids of strain SS2-56T were phosphatidylethanolamine, one unidentified aminophospholipid and four unidentified lipids. The DNA G+C content was 43.8 mol%. Phenotypic, chemotaxonomic and phylogenetic evidence clearly indicates that strain SS2-56T represents a novel species of the genus Lacibacter, for which the name Lacibacter nakdongensis sp. nov. is proposed. The type strain is SS2-56T (=KCTC 52160T=JCM 31372T).

Lacibacter sediminis sp. nov., isolated from contaminated freshwater sediment

“…S3), with a DNA G+C content of 40.3 %, was assembled using an Oxford Nanopore device and was deposited at DDBJ/EMBL/GenBank with the accession number CP060007. The G+C content of the genomic DNA was determined to be 40.2–40.3 %, which is a little lower than the range of values for the members of the genus Lacibacter (40.6–46.6 %) [1, 3–5]. The complete and closed genome of S13-6-22 T contained a total of 4314 genes, of which 4260 were coding and 51 were ribosomal or transfer RNAs (Table S1).…”

“…The members of the genus Lacibacter are Gram-stain-negative, aerobic, non-spore forming, rod-shaped, motile and chemotaxonomically possess MK-7 as the primary quinone, phosphatidylethanolamine as the major polar lipid and iso-C 15 : 0 , iso-C 15 : 1 G, iso-C 17 : 0 3-OH and summed feature 3 (C 16 : 1 ω7c and/or C 16 : 1 ω6c) as the predominant fatty acids. The G+C content of the genomic DNA is within the range between 40.6 and 46.6 mol% [1, 3–5]. The genus Lacibacter taxonomically belongs to the family Chitinophagaceae , which Kampfer et al [6] first ascribed to the phylum Bacteroidota and represents a phylogenetically diverse family.…”

Toward a More Comprehensive Understanding of Autochthonous Bioaugmentation (ABA): Cases of ABA for Phenanthrene and Biphenyl by Ralstonia sp. M1 in Industrial Wastewater

“…46 The genus Lacibacter (family Chitinophagaceae) was proposed by Qu et al and characterized as a Gram-staining-negative, aerobic, rod-shaped bacterium. 47 This genus comprises only three species (Lacibacter cauensis, Lacibacter daechungensis, and Lacibacter nakdongensis) 48 and can degrade some common carbon sources such as starch, xanthine, and tyrosine. 48 However, PAH biodegradation of the genus Lacibacter and BP and PCB biodegradation of the family Chitinophagaceae have not been previously reported, and the ability of these bacterial taxa to metabolize PAHs or BPs and PCBs is unknown.…”

“…47 This genus comprises only three species (Lacibacter cauensis, Lacibacter daechungensis, and Lacibacter nakdongensis) 48 and can degrade some common carbon sources such as starch, xanthine, and tyrosine. 48 However, PAH biodegradation of the genus Lacibacter and BP and PCB biodegradation of the family Chitinophagaceae have not been previously reported, and the ability of these bacterial taxa to metabolize PAHs or BPs and PCBs is unknown. Cytophagaceae was one of the dominant groups detected in soils at industrial sites, 49 but it is rarely associated with the degradation of PAHs.…”