Role of NCAN rs2228603 polymorphism in the incidence of nonalcoholic fatty liver disease: a case-control study

Abstract

BackgroundRecently genome-wide association studies identified that NCAN rs2228603 polymorphism was associated with non-alcoholic fatty liver disease (NAFLD) mainly in subjects of European ancestry. While no research have been conducted to demonstrate the relationship between NCAN rs2228603 and NAFLD in Chinese Han adults. The aim of this study was to investigate whether NCAN rs2228603 is associated with NAFLD in Chinese population.MethodsGene NCAN rs2228603 was genotyped in 182 patients with NAFLD and 195 healthy controls. The expression of NCAN was tested according to polymerase chain reaction analysis (PCR) and serum lipids were performed by biology techniques.ResultsNo significant difference was found in genotype and allele frequencies of NCAN rs2228603 between the NAFLD group and the controls (P > 0.05). Subjects with the NCAN rs2228603 CT genotype showed a higher level of alkaline phosphatase (AKP) (P = 0.017) and a higher high-density lipoprotein (HDL) (P < 0.05).ConclusionsOur study for the first time identified that the gene NCAN rs2228603 is not a risk factor for the incidence of NAFLD in Chinese population. Also we found the dual and opposite role of T variant in protecting liver with a higher level of HDL and conferring risk for liver damage with a higher level of AKP.Trial registrationChinese Clinical Trial Register.gov Identifier: ChiCTR-ROC-15006447.

Profiling the genome and proteome of metabolic dysfunction-associated steatotic liver disease identifies potential therapeutic targets

“…Our study provides causal evidence for previous MASLD targets, including ANPEP, APOC1, APOE, IL1RN, KRT18, KRT8, LPL, NCAN, and SORT1 [63][64][65][66][67][68][69] . Notably, there have been approved drugs targeting ANPEP, APOE, LPL and SORT1 48,49 , though their effect on liver diseases is still not clear.…”

Associations of Hydroxysteroid 17-beta Dehydrogenase 13 Variants with Liver Histology in Chinese Patients with Metabolic-associated Fatty Liver Disease

“…The differential effect size and allele direction of variants discovered by GWAS between ethnicities is not uncommon. In one Chinese MAFLD cohort, researchers found that the neurocan (known as NCAN ) rs2228603 T variant associated with a higher level of high-density lipoprotein, 21 while it was positively related to liver steatosis in the USA population. 22 Similarly, toll-like receptor 3 (known as TLR3 ) rs3775290 23 , 24 and interferon lambda-3 (known as IFNL3 ) rs12979860 25 , 26 variants in Chinese hepatocellular carcinoma populations showed opposite effects to those in non-Asian populations.…”