A facile and green strategy for the synthesis of Au, Ag and Au–Ag alloy nanoparticles using aerial parts of R. hypocrateriformis extract and their biological evaluation

Godipurge, Shivakumar S.; Yallappa, S.; Biradar, Naveen J.; Biradar, J. S.; Dhananjaya, Bhadrapura Lakkappa; Hegde, Gajanan G.; Jagadish, K.; Hegde, Gurumurthy

doi:10.1016/j.enzmictec.2016.08.006

Cited by 90 publications

(45 citation statements)

References 46 publications

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“… 34 Todays, there are many plants which are used for synthesizing metal nanoparticles such as Ag. 35 , 36 However, only one of these species, S.rechengri is used for AgNPs synthesis although Satureja has many species. Biological organisms are significant at both capping and oxidant agents in the synthesis of AgNPs.…”

Section: Resultsmentioning

confidence: 99%

Phytochemical Synthesis of Silver Nanoparticles by Two Techniques Using Saturaja rechengri Jamzad Extract: Identifying and Comparing in Vitro Anti-Proliferative Activities

Narchin¹,

Larijani²,

Rustaiyan³

et al. 2018

Adv Pharm Bull

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Purpose: A lot of plants are available which can produce nanoparticles used in medicine, life sciences, and the pharmaceutical industry. The present study aims to introduce safe biological and eco-friendly methods for synthesizing silver nanoparticles (AgNPs) by using Saturaja rechengri Jamzad extract, which can replace traditional chemical methods. In addition, the chemical nature and antimicrobial activities were identified and accordingly the anticancer effects of AgNPs was successfully reported on colon cancer cells (HT-29).Methods: Light and ultrasound, as two green chemistry techniques were first used for AgNPs synthesis. Then, morphological and crystalline structure of AgNPs was evaluated by scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis, respectively. In addition, functional groups were determined by using the Fourier transform infrared spectroscopy (FTIR) spectrum. Further, a maximum adsorption of AgNPs was observed in UV-visible spectrum. In the next stage, antibacterial activity of green synthesized AgNPs was evaluated against two pathogenic bacteria including Escherichia coli and Staphylococcus aureus. Finally, the cytotoxicity of AgNPs on HT-29 at different concentrations and times of AgNPs was determined by MTT assay.Results: The findings indicated that the synthesis of AgNPs by ultrasonic technique leads to smaller particle size and more distribution. Based on the results of MTT test for calculating the IC50%, the anti-proliferative effects of the light and ultrasound AgNPs were observed on HT-29 cell lines depending on the dose and time. Finally, the AgNPs had the most cytotoxicity HT-29 cell lines at 100 μg/ml concentration although the lowest toxicity effect was reported on HEK-293 cell lines at the same conditions.Conclusion: The change in the concentration, physical and chemical properties of AgNPs including the form and size of particles, and their type of covering and fields can influence the induction of cytotoxicity and morphological change in the treated cells. The present research opens a new horizon on the development of new biological and cytotoxicity agents.

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Section: Resultsmentioning

confidence: 99%

Phytochemical Synthesis of Silver Nanoparticles by Two Techniques Using Saturaja rechengri Jamzad Extract: Identifying and Comparing in Vitro Anti-Proliferative Activities

Narchin¹,

Larijani²,

Rustaiyan³

et al. 2018

Adv Pharm Bull

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“…The peaks at 1408.57 cm −1 (CNL) and 1410.77 cm −1 (CNS) for both plant extracts shifted to 1382.47 cm −1 (AgNP-L) and 1383.51 cm −1 (AgNP-S). This corresponds to the C-O-H bending (in-plane) of carboxylic acids vibrations [75]. The peaks of both C. nutans extracts at 1093.41 and 1043.45 cm −1 show the major reductions on the AgNPs FTIR spectra.…”

Section: Fourier Transform Infrared Spectrophotometer Spectroscopy Anmentioning

confidence: 94%

Optimization of biogenic synthesis of silver nanoparticles from flavonoid-rich Clinacanthus nutans leaf and stem aqueous extracts

et al. 2020

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Background : Silver nanoparticles (AgNPs) are widely used in food industries, biomedical, dentistry, catalysis, diagnostic biological probes and sensors. The use of plant extract for AgNPs synthesis eliminates the process of maintaining cell culture and the process could be scaled up under a non-aseptic environment. The purpose of this study is to determine the classes of phytochemicals, to biosynthesize and characterize the AgNPs using Clinacanthus nutans leaf and stem extracts. In this study, AgNPs were synthesized from the aqueous extracts of C. nutans leaves and stems through a non-toxic, cost-effective and eco-friendly method. Results : The formation of AgNPs was confirmed by UV-Vis spectroscopy, and the size of AgNP-L (leaf) and AgNP-S (stem) were 114.7 and 129.9 nm, respectively. Transmission electron microscopy (TEM) analysis showed spherical nanoparticles with AgNP-L and AgNP-S ranging from 10 to 300 nm and 10 to 180 nm, with average of 101.18 and 75.38 nm, respectively. The zeta potentials of AgNP-L and AgNP-S were recorded at −42.8 and −43.9 mV. X-ray diffraction analysis matched the face-centred cubic structure of silver and was capped with bioactive compounds. Fourier transform infrared spectrophotometer analysis revealed the presence of few functional groups of phenolic and flavonoid compounds. These functional groups act as reducing agents in AgNPs synthesis. Conclusion : These results showed that the biogenically synthesized nanoparticles reduced silver ions to silver nanoparticles in aqueous condition and the AgNPs formed were stable and less toxic.

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“…A. atropatana is cheap and easily available and there is no need to capping and reducing agent. Recently, many plant extracts have been studied for the synthesis of AgNPs [22,23] but, the extract from aerial part of A. atropatana have not been used for green synthesis of AgNPs, so far. For UV-vis analysis, the absorption of NPs was observed in 430 nm wavelength in UV-vis spectrum.…”

Section: Synthesis and Characterization Of Agnpsmentioning

confidence: 99%

Green synthesis of silver nanoparticles using Anthemis atropatana extract: characterization and in vitro biological activities

Dehghanizade

Arasteh

Mirzaie

2017

Artificial Cells, Nanomedicine, and Biotechnology

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This study aim to synthesize silver nanoparticles (AgNPs) using Anthemis atropatana extract and to evaluate their chemical characteristics and antimicrobial and cytotoxic effects. The biosynthesis of AgNPs is verified using UV-visible spectrum which showing maximum absorption in 430 nm wavelength. Transmission electron microscope (TEM) and scanning electron microscope (SEM) results revealed that AgNPs has a spherical shape with an average size of 38.89 nanometres. The crystalline structure of green synthesized AgNPs in optimal conditions was confirmed by XRD analysis. The pattern of XRD peaks related to face-centred cubic (fcc) (111), (200), (220), (311) and (222) observed. Also, FTIR results verified the AgNPs synthesis using plant extract. In biological tests, the MTT results indicate the dose dependence of cytotoxic effects of AgNPs on colon cancer cell lines (HT29). The AgNPs had maximum cytotoxicity on HT29 cancer cell line at 100 μg/ml concentration, which were statistically significant comparing control cells (p < .001). Moreover, real time PCR and flow cytometry results confirmed the apoptotic effects of AgNPs. According to the results, it seems that the green synthesis of AgNPs is an eco-friendly and cost effective approach. This research provides insight into the development of new anticancer and antibacterial agents.

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A facile and green strategy for the synthesis of Au, Ag and Au–Ag alloy nanoparticles using aerial parts of R. hypocrateriformis extract and their biological evaluation

Cited by 90 publications

References 46 publications

Phytochemical Synthesis of Silver Nanoparticles by Two Techniques Using Saturaja rechengri Jamzad Extract: Identifying and Comparing in Vitro Anti-Proliferative Activities

Phytochemical Synthesis of Silver Nanoparticles by Two Techniques Using Saturaja rechengri Jamzad Extract: Identifying and Comparing in Vitro Anti-Proliferative Activities

Optimization of biogenic synthesis of silver nanoparticles from flavonoid-rich Clinacanthus nutans leaf and stem aqueous extracts

Green synthesis of silver nanoparticles using Anthemis atropatana extract: characterization and in vitro biological activities

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