Detection of Helicobacter pylori and the genotypes of resistance to clarithromycin and the heterogeneous genotype to this antibiotic in biopsies obtained from symptomatic children

Aguilera-Correa, John Jairo; Urruzuno, Pedro; Barrio, Josefa; Martínez, María José Fresnadillo; Agudo, Sonia; Somodevilla, Angela; Llorca, Laura; Alarcón, Teresa

doi:10.1016/j.diagmicrobio.2016.03.001

Cited by 29 publications

(20 citation statements)

References 26 publications

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“…A2143G mutation has a higher risk of eradication failure [15,16,21,25]. This mutation demonstrated the highest rate among examined mutations, in accordance with other studies [14,22,24].…”

Section: Discussionsupporting

confidence: 90%

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Phenotypic and genotypic correlation as expressed in Helicobacter pylori resistance to clarithromycin and fluoroquinolones

Binyamin

Pastukh

et al. 2017

Gut Pathog

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Background:Helicobacter pylori susceptibility to clarithromycin and fluoroquinolone can be determined through Etest or molecular assays. We examined the correlation between phenotypic susceptibility (MIC results) and genotypic susceptibility in H. pylori strains isolated from gastric biopsies. Results:Out of 85 duplicate biopsies obtained from patients from northern Israel who underwent endoscopy, 70 were positive for H. pylori using the CUTest (urease test). These biopsies were cultured for H. pylori and Etest breakpoints were determined for levofloxacin and clarithromycin. H. pylori detection, characterization of wild type (WT) or mutant alleles, and antibiotic resistance were determined molecularly using GenoType HelicoDR kit. Phenotypic and genotype results were compared. The molecular method had higher sensitivity for detection of H. pylori than culture (94.3% vs. 77.1%). Resistance to clarithromycin was higher than to levofloxacin by both methods. Levofloxacin resistance was found in 21.2 and 0% using genotypic and phenotypic methods, respectively. Clarithromycin resistance was found in 66.7 and 62.9% by genotypic and phenotypic methods, respectively. Some samples were found susceptible by Etest and resistant by GenoType HelicoDR (13/53 samples) and some vice versa (10/53 samples). Conclusion:GenoType HelicoDR kit has high sensitivity rate for H. pylori detection. It has the ability to detect many mutations and can help to determine initial antibiotic treatment at the beginning of therapy. However, there were a few cases where there was a phenotype-genotype mismatch result that could derive from possible causes. For example, a resistance mechanism that is not tested in the kit.

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“…A2143G mutation has a higher risk of eradication failure [15,16,21,25]. This mutation demonstrated the highest rate among examined mutations, in accordance with other studies [14,22,24].…”

Section: Discussionsupporting

confidence: 90%

“…However, some differences between phenotype and genotype profiles were observed; these were characterized by phenotypic susceptibility and genotypic resistance or vice versa, in contrast to other studies [22][23][24].…”

Section: Discussioncontrasting

confidence: 85%

Phenotypic and genotypic correlation as expressed in Helicobacter pylori resistance to clarithromycin and fluoroquinolones

Binyamin

Pastukh

et al. 2017

Gut Pathog

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“…In 11 biopsies, wild-type plus A2143G or A2142G were found. 41 However, the authors did not investigate the possibility of multiple infections that may also explain these findings.…”

Section: Resistance To Antibioticsmentioning

confidence: 96%

“…The most frequent mutation was A2143G (87.5%), followed by A2142G (7.5%) and double mutant A2142C‐A2143G (5%). In 11 biopsies, wild‐type plus A2143G or A2142G were found . However, the authors did not investigate the possibility of multiple infections that may also explain these findings.…”

Section: Resistance To Antibioticsmentioning

confidence: 96%

Helicobacter pylori infection in children

2017

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Helicobacter pylori infection in children differs from that in adults, from the point of view of epidemiology, host response, clinical features, related diseases, and diagnosis, as well as treatment strategies. The prevalence of H. pylori infection, in both children and adults, is decreasing in the Western World as well as in some developing countries, which contrasts with the increase in childhood asthma and allergic diseases. Recurrent abdominal pain is not specific during H. pylori infection in children. The role of H. pylori infection and failure to thrive, children's growth, type I diabetes mellitus (T1DM) and celiac disease remains controversial. The main initial diagnosis is based on upper digestive endoscopy with biopsy-based methods. Nodular gastritis may be a pathognomonic endoscopic finding of childhood H. pylori infection. The infection eradication control is based on validated noninvasive tests. The main cause of treatment failure of H. pylori infection is its clarithromycin resistance. We recommend standard antibiotic susceptibility testing of H. pylori in pediatric patients prior to the initiation of eradication therapy. H. pylori treatment in children should be based on an evaluation of the rate of eradication in the local population, a systematic use of a treatment adapted to the susceptibility profile and a treatment compliance greater than 90%. The last meta-analysis in children did not show an advantage for sequential therapy when compared to a 14-day triple therapy. Finally, the high rate of antibiotic resistance responsible for therapy failure in recent years justifies the necessity of a novel vaccine to prevent H. pylori infection in children.

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“…Desde hace muchos años se han utilizado métodos moleculares caseros para el diagnóstico de la infección por H. pylori a partir de diferentes muestras como biopsia gástrica, heces, saliva, jugo gástrico, etc. Además, permiten el estudio de mutaciones de resistencia a macrólidos como claritromicina (gen 23S ARNr), fluoroquinolonas (gen gyrA), tetraciclinas (gen 16S ARNr), rifabutina (gen rpoB) y amoxicilina (gen pbp-1a) 41,42 , y tienen las ventajas de la rapidez, de la mayor sensibilidad que el cultivo con métodos fenotípicos de sensibilidad a antibióticos, y detectan resistencia heterogénea, que puede ser difícil de obtener por cultivo 39,43 .…”

Section: Helicobacter Pyloriunclassified

Diagnóstico rápido de las infecciones del tracto gastrointestinal por parásitos, virus y bacterias

Balsalobre-Arenas

Alarcón-Cavero

2017

Enfermedades Infecciosas y Microbiología Clínica

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Rapid diagnostic techniques are valuable tools in the diagnosis of gastrointestinal infections, especially for the detection of some microorganisms and in certain groups of patients. While antigen detection techniques are widely used in Clinical Microbiology laboratories, for the diagnosis of viruses, some parasites and some bacteria, molecular techniques are routinely used only for some pathogens (such as Clostridium difficile). However, molecular techniques are constantly evolving, and they allow a rapid diagnosis for an increasing number of pathogens, with high sensitivity and specificity. In addition, they are also able to detect virulence factors or resistance mechanisms. Syndromic surveillance systems, which detect different pathogens simultaneously, are very promising because they enable the most frequent pathogens to be diagnosed in a few hours and they can be very useful in certain patients. For the diagnosis of Helicobacter pylori infection, molecular techniques are able to detect bacteria and its resistance to clarithromycin and levofloxacin, allowing the most appropriate treatment to be selected for each patient when bacterial culture is not possible.

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Detection of Helicobacter pylori and the genotypes of resistance to clarithromycin and the heterogeneous genotype to this antibiotic in biopsies obtained from symptomatic children

Cited by 29 publications

References 26 publications

Phenotypic and genotypic correlation as expressed in Helicobacter pylori resistance to clarithromycin and fluoroquinolones

Phenotypic and genotypic correlation as expressed in Helicobacter pylori resistance to clarithromycin and fluoroquinolones

Helicobacter pylori infection in children

Diagnóstico rápido de las infecciones del tracto gastrointestinal por parásitos, virus y bacterias

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