Comparison of Chemical Compositions in Pseudostellariae Radix from Different Cultivated Fields and Germplasms by NMR-Based Metabolomics

Hua, Yujiao; Hou, Yunlong; Wang, Shengnan; Ma, Yang; Liu, Zixiu; Zou, Lisi; Liu, Xunhong; Luo, Yi‐Bo; Liu, Juanxiu

doi:10.3390/molecules21111538

Cited by 12 publications

(6 citation statements)

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“…Two parameters, R 2 Y and Q 2 , were used to evaluate the model, the closer R 2 Y is to 1, the more stable the model is, and Q 2 > 0.5 indicates a high prediction rate. Variable importance in the projection (VIP) > 1 via OPLS-DA analysis and the p value of the t -test ( p < 0.05) were used to find potential metabolites that significantly contributed to the difference among the groups [ 21 ]. Biochemical databases, METLIN ( http://metlin.scripps.edu/ ), HMDB ( http://www.hmdb.ca/ ), KEGG ( http://www.kegg.com/ ), and MetaboAnalyst ( http://www.metaboanalyst.ca/ ), were used to identify potential metabolites.…”

Section: Methodsmentioning

confidence: 99%

Comparison of Chemical Constituents in Pseudostellariae Radix with Different Dosage Forms Based on HPLC-Q-Exactive Orbitrap/MS Combined with Multivariate Statistical Analysis

Hua

Liu

Xie

2021

Evidence-Based Complementary and Alternative Medicine

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Background. Pseudostellariae Radix (PR) is an important traditional Chinese herbal medicine with vast clinical consumptions, which has two different dosage forms, PR decoction pieces and PR formula granules. However, these two forms are bound to have an impact on the accumulation of the effective components in PR, so the effectiveness of clinical use cannot be guaranteed. Objective. To determine the effective composition of PR. Methods. In this research, PR decoction pieces and formula granules were collected, and their composition was detected by HPLC-Q-Exactive Orbitrap/MS; multivariate statistical analysis was used to distinguish differential metabolites between PR decoction pieces and formula granules. Results. A clear cut difference in the composition of the two groups was observed. 98 differential chemical constituents could be identified in the positive mode, while 52 differential chemical compositions could be identified in the negative mode. The differential chemical compositions were mainly concentrated in flavonoids, organic acids, fatty acids, and amino acids and present different change rules, mainly involved in the isoquinoline alkaloid biosynthesis metabolic pathways. Conclusions. This study provides basic information to reveal the influence law of different dosage forms on the metabolite synthesis and quality formation mechanism of PR.

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Section: Methodsmentioning

confidence: 99%

Comparison of Chemical Constituents in Pseudostellariae Radix with Different Dosage Forms Based on HPLC-Q-Exactive Orbitrap/MS Combined with Multivariate Statistical Analysis

Hua

Liu

Xie

2021

Evidence-Based Complementary and Alternative Medicine

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“… UPLC-triple TOF–MS/MS [ 44 – 46 ] 21 compounds Ultra-performance liquid chromatography-triple time-of-flight mass/mass spectrometry (UPLC-triple TOF–MS/MS) [ 70 ] 34 components (heterophyllin A and B, alanine, lactate, lysine, taurine, sucrose, tyrosine, linolenic acid, γ-aminobutyrate, glutamine, raffinose, xylose etc.) 1H-NMR-based metabolomics coupled with HPLC [ 47 ] Free amino acid NIR [ 71 ] Nucleosides and nucleobases QTRAP LC–MS/MS [ 55 – 57 , 72 ] Volatile components: palmitic acid (21.37%), 9,12-octadecadienoic acid ethylester (16.98%), trans-oleic acid (5.94%), chondrillasterol (3.99%), stigmast-7-en-3-ol (3.92%), 5,6-dihydroergosterol(2.48%), 1-monolinolein (2.35%) GC–MS [ 73 ] Polysaccharide High-performance size-exclusion chromatograph (HPSEC) [ 74 ] Water-soluble sugar Phenol–sulfuric acid [ 75 ] Fingerprint HPLC [ 16 , 37 , 58 – 60 , 76 – 78 ] Fingerprint GC–MS [ 79 ] …”

Section: Chemical Analysis Of P Heterophyllamentioning

confidence: 99%

“…The other studies focused on cyclic peptides were for simultaneous analysis of pseudostellarin A, C, D, and G using HPLC coupled with electrospray ionization tandem mass spectrometry (ESI–MSn) [ 25 ], and UPLC- quadrupole time of flight (QTOF)-MS/MS methods for analysis of pseudostellarin A, B, D, F, and heterophyllin A in P. heterophylla [ 44 – 46 ]. 1H-NMR-based metabolomics coupled with HPLC was also employed to investigate the metabolites in P. heterophylla [ 47 ], which has the unique advantages in the accurate identification of components.…”

Section: Chemical Analysis Of P Heterophyllamentioning

confidence: 99%

“…Near infrared (NIR) spectroscopy combined with support vector data description (SVDD) was attempted to identify the geographical origins of P. heterophylla [ 6 ]. NMR has also been developed for identification of wild P. heterophylla from different cultivated fields [ 47 , 53 ]. In addition, high-throughput RNA sequencing (RNA-seq) was employed as de novo assembly for studying the transcriptome in P. heterophylla , and significantly differentially expressed genes in P. heterophylla from different fields were found [ 54 ].…”

Section: Chemical Analysis Of P Heterophyllamentioning

confidence: 99%

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Chemistry, pharmacology and analysis of Pseudostellaria heterophylla: a mini-review

Shakerian

Zhao

et al. 2019

Chin Med

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Pseudostellaria heterophylla is one of the well-known traditional Chinese medicines and has been used in clinics for 100 years in China. The chemistry and pharmacology of P. heterophylla were reviewed to understand its active compounds. Then analysis of these compounds related to quality control of this herb was discussed. For the analysis of chemicals, three aspects have been discussed in this review. The first two aspects focused on the methodologies for analysis of cyclic peptides and carbohydrates in P. heterophylla, respectively. The last one dealt with the other methods used for identification of P. heterophylla. Some rich chemicals such as oligosaccharides in this plant were rarely evaluated. Many analyses were performed on this plant, however, few of them were accepted as quality control method.

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“…Pax (Morita et al, 1995; Han et al, 2008). P. heterophylla is an herbaceous perennial, a Caryophyllaceae species known as Taizishen (Hua et al, 2016a). Its dried tuberous root is named Pseudostellariae radix and is a traditional Chinese medicine with high pharmacodynamic value.…”

Section: Introductionmentioning

confidence: 99%

The Biosynthesis of Heterophyllin B in Pseudostellaria heterophylla From prePhHB-Encoded Precursor

Zheng

Zhou

et al. 2019

Front. Plant Sci.

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Plant cyclic peptides (CPs) are a large group of small molecule metabolites found in a wide variety of plants, including traditional Chinese medicinal plants. However, the majority of plant CPs have not been studied for their biosynthetic mechanisms, including heterophyllin B (HB), which is one of the characteristic chemical components of Pseudostellaria heterophylla. Here, we screened the precursor gene (prePhHB) of HB in P. heterophylla and functionally identified its correctness in vivo and in vitro. First, we developed a new method to screen the precursors of HB from 16 candidate linear peptides. According to transcriptome sequencing data, we cloned the genes that encoded the HB precursor peptides and confirmed that the prePhHB-encoded precursor peptide could enzymatically synthesize HB. Next, we generated the transgenic tobacco that expressed prePhHB, and the results showed that HB was detected in transgenic tobacco. Moreover, we revealed that prePhHB gene expression is positively correlated with HB accumulation in P. heterophylla. Mutations in the prePhHB gene may influence the accumulation of HB in P. heterophylla. These results suggest that HB is ribosomally synthesized and posttranslationally modified peptide (RiPP) derived from the precursor gene prePhHB-encoded precursor peptide, and the core peptide sequence of HB is IFGGLPPP in P. heterophylla. This study developed a new idea for the rapid identification of Caryophyllaceae-type CP precursor peptides via RNA-sequencing data mining.

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Comparison of Chemical Compositions in Pseudostellariae Radix from Different Cultivated Fields and Germplasms by NMR-Based Metabolomics

Cited by 12 publications

References 12 publications

Comparison of Chemical Constituents in Pseudostellariae Radix with Different Dosage Forms Based on HPLC-Q-Exactive Orbitrap/MS Combined with Multivariate Statistical Analysis

Comparison of Chemical Constituents in Pseudostellariae Radix with Different Dosage Forms Based on HPLC-Q-Exactive Orbitrap/MS Combined with Multivariate Statistical Analysis

Chemistry, pharmacology and analysis of Pseudostellaria heterophylla: a mini-review

The Biosynthesis of Heterophyllin B in Pseudostellaria heterophylla From prePhHB-Encoded Precursor

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