Adipose tissue gene expression is differentially regulated with different rates of weight loss in overweight and obese humans

Abstract

The rate of WL (LCD vs VLCD), with similar total WL, strongly regulates AT gene expression. Increased mitochondrial function, angiogenesis and adipogenesis on a VLCD compared with a LCD reflect potential beneficial diet-induced changes in AT, whereas differential neuronal and olfactory regulation suggest functions of these genes beyond the current paradigm.

Prioritizing putative influential genes in early life cardiovascular disease susceptibility by applying tissue-specific Mendelian randomization

“…found that the magnitude of the effect for ADCY3 expression was observed most strongly 369 in adipose tissue, aligning with other research [50,51]. Furthermore, recent work has 370 uncovered a variant in ADCY3 associated with an increase in obesity levels [52].…”

Adipose Tissue Meal‐Derived Fatty Acid Uptake Before and After Diet‐Induced Weight Loss in Adults with Overweight and Obesity

“…Sample preparation and gene expression analysis were performed as described previously . In short, total RNA (100 ng per sample) was labeled by Whole Transcript Sense Target (ST) Labeling Assay Assay and hybridized to whole‐genome Human Gene 1.1 ST arrays targeting 19,654 unique genes (Affymetrix, Santa Clara, California).…”

eXplainable Artificial Intelligence (XAI) for the identification of biologically relevant gene expression patterns in longitudinal human studies, insights from obesity research

“…With up to three time records available in the dataset, this study constitutes a perfect example of the in vivo temporal microarray experiments in which our method could extract biologically relevant gene-gene temporal relationships. Published by Vink et al (2016) [47], the original clinical trial investigated the effects on weight loss (WL) of two different dietary interventions in 57 adults with obesity. Subjects were randomly assigned to each experimental group: a low-calorie diet (LCD; 1250 kcal/day) for 12 weeks (slow weight loss) or a very-low-calorie diet (VLCD; 500 kcal/day) for 5 weeks (rapid weight loss).…”

“…As we previously explained, with the aim of illustrating the performance of our method on human long-term intervention data, we accessed and downloaded a discovery dataset composed of 57 subjects with obesity participating in a long-term dietary program [47,48]. The dataset consisted on temporal GED collected in three different time records during the course of two dietary interventions (VLCD and LCD).…”