“…The primary antibodies used are as follows: mouse anti-α-spectrin 3A9 (1:500, DSHB), rat anti-Vasa (1:10, DSHB), mouse anti-fasciclin III 7G10 (1:50, DSHB), rabbit anti-cleaved caspase 3 (1:100, Cell Signaling, 9661S), rabbit anti-phospho-Smad5 Ser463/465 (referred to as pMad, 1:100, Epitomic, 2224-1, a kind gift from L. De Koning, Institut Curie, Paris, France), rabbit anti-γH2Av (1:1000, Rockland, 600-401-914), rabbit anti-P180 [1:1000, antibody raised in rabbit against a peptide corresponding to the residues 198-421 of P180 (Roelens et al, 2017)], rabbit anti-fibrillarin (1:500, Abcam), rabbit anti-I-eleORF (1:400, kindly provided by D. Finnegan, Edinburgh University, UK), rabbit anti-H3 (1:200, Abcam, 1791) and rabbit anti-aubergine (1:500, kindly provided by P. Lasko, McGill University, Montreal, Canada). Alexa Fluor 488-, 568-or 633conjugated secondary antibodies (Life Technologies and Jackson Immunoresearch) were used at a 1:500 dilution.…”