Effect of the herbicide atrazine and its metabolite DACT on bovine sperm quality

Komsky-Elbaz, Alisa; Roth, Zvi

doi:10.1016/j.reprotox.2016.11.001

Cited by 32 publications

(11 citation statements)

References 56 publications

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“…15 An in vitro study has investigated the effect of atrazine (ATZ), a chlorotriazine herbicide and its metabolite, diaminochlorotriazine (DACT). 16 In another study, intraperitoneal ATZ was given to Fischer rats twice a week for 60 days. Reduced sperm motility was found, but there was an increase in sperm number.…”

Section: Pesticidesmentioning

confidence: 99%

Endocrine‐disrupting chemicals and male reproductive health

Sharma

Mollier

Brocklesby

et al. 2020

Reprod Medicine & Biology

100

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A number of studies have reported a deterioration in sperm quality over the past 50 years, 1,2 and a variety of factors have been implicated in this decline, including environmental and lifestyle factors. Data from animal and human studies suggest that endocrine-disrupting chemicals are associated in the etiopathogenesis with harmful effects on male reproductive health. 3 Furthermore, recent meta-analysis has suggested that EDCs not only have a deleterious effect on sperm quality but may also be associated with cryptorchidism, hypospadias, and testis cancer, the so-called testicular dysgenesis syndrome. 4 It has been postulated that environmental chemicals may affect the endocrine regulation of fertility since the 1920s. 5 Over the past decade, many chemicals have been observed to have the ability to disrupt the endocrine system and male reproductive health.

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Section: Pesticidesmentioning

confidence: 99%

Endocrine‐disrupting chemicals and male reproductive health

Sharma

Mollier

Brocklesby

et al. 2020

Reprod Medicine & Biology

100

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“…Plasma membrane Damage to plasma membrane integrity may reduce spermatozoa's viability and thus reduce their fertilization capacity ( Komsky-Elbaz and Roth, 2017 ). Various EDCs, such as BPA and phthalates, have been reported to decrease spermatozoon viability in humans ( Pant et al., 2011 ; Knez et al., 2014 ), and the herbicide, fenoxaprop-ethyl, in porcine ( Betancourt et al., 2006 ).…”

Section: Effect Of Foodborne Contaminants On Male Reproductionmentioning

confidence: 99%

Effect of environmental contamination on female and male gametes – A lesson from bovines

2020

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Endocrine-disrupting compounds (EDCs) and foodborne contaminants are environmental pollutants that are considered reproductive toxicants due to their deleterious effects on female and male gametes. Among the EDCs, the phthalate plasticizers are of growing concern. In-vivo and in-vitro models indicate that the oocyte is highly sensitive to phthalates. This review summarizes the effects of di(2-ethylhexyl) phthalate and its major metabolite mono(2-ethyhexyl) phthalate (MEHP) on the oocyte. MEHP reduces the proportion of oocytes that fertilize, cleave and develop to the blastocyst stage. This is associated with negative effects on meiotic progression, and disruption of cortical granules, endoplasmic reticulum and mitochondrial reorganization. MEHP alters mitochondrial membrane polarity, increases reactive oxygen species levels and induces alterations in genes associated with oxidative phosphorylation. A carryover effect from the oocyte to the blastocyst is manifested by alterations in the transcriptomic profile of blastocysts developed from MEHP-treated oocytes. Among foodborne contaminants, the pesticide atrazine (ATZ) and the mycotoxin aflatoxin B1 (AFB1) are of high concern. The potential hazards associated with exposure of spermatozoa to these contaminants and their carryover effect to the blastocyst are described. AFB1 and ATZ reduce spermatozoa's viability, as reflected by a high proportion of cells with damaged plasma membrane; induce acrosome reaction, expressed as damage to the acrosomal membrane; and interfere with mitochondrial function, characterized by hyperpolarization of the membrane. ATZ and AFB1-treated spermatozoa show a high proportion of cells with fragmented DNA. Exposure of spermatozoa to AFB1 and ATZ reduces fertilization and cleavage rates, but not that of blastocyst formation. However, fertilization with AFB1-or ATZ-treated spermatozoa impairs transcript expression in the formed blastocysts, implying a carryover effect. Taken together, the review indicates the risk of exposing farm animals to environmental contaminants, and their deleterious effects on female and male gametes and the developing embryo.

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“…Dapper, antagonist of β-catenin (Dact) gene is a signal pathway regulating molecule that exerts its function by negatively regulating Wnt/β-catenin and Nodal/TGFβ signaling pathways (7,8). Wnt/β-catenin and Nodal/TGFβ signaling pathways participate in the regulation of the occurrence and development of tumors, and Dact gene can therefore play a role in the occurrence and development of tumors by affecting these signaling pathways (9).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑214 promotes the proliferation, migration and invasion of gastric cancer MKN28 cells by suppressing the expression of Dact2

Zhao

Fan

et al. 2018

Exp Ther Med

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The present study examined the expression of Dapper, antagonist of β-catenin 2 (Dact2) and microRNA (miR)-214 in gastric cancer at tissue and cellular levels, and to understand their biological roles. A total of 42 gastric cancer patients were enrolled in the present study. Bioinformatics tool was used to predict the miR molecule that potentially regulates Dact2 expression. To measure the expression of miR-214 and Dact2, reverse transcription-quantitative polymerase chain reaction was employed. Mixed gastric adenocarcinoma type MKN28 cells were transfected with negative control (NC), miR-214 mimics or inhibitor. The CCK-8 assay was used to investigate the proliferation of mixed gastric adenocarcinoma type MKN28 cells. To study migration and invasion abilities of mixed gastric adenocarcinoma type MKN28 cells, the Transwell assay was performed. To determine the expression of Dact2 protein, western blotting was conducted and the rescue assay was utilized to study the biological roles of miR-214 and Dact2 in mixed gastric adenocarcinoma type MKN28 cells. To test whether Dact2 is a direct target of miR-214, the dual luciferase reporter assay was performed. Results indicated that the expression of miR-214 was elevated, but expression of Dact2 mRNA was decreased in gastric cancer tissues, which was closely correlated with the invasion, metastasis, occurrence and development of gastric cancer. Notably, miR-214 promoted the proliferation of mixed gastric adenocarcinoma type MKN28 cells in vitro, whereas but Dact2 inhibited the proliferation of these cells. Downregulation of miR-214 expression or upregulation of Dact2 expression inhibited the migration and invasion of mixed gastric adenocarcinoma type MKN28 cells. Furthermore, miR-214 regulated the expression of Dact2 protein and its downstream β-catenin protein in mixed gastric adenocarcinoma type MKN28 cells. Dact2 reversed the effect of miR-214 on the proliferation, migration and invasion of mixed gastric adenocarcinoma type MKN28 cells. In addition, miR-214 directly targeted the 3'-UTR seeding region of Dact2 mRNA to regulate its expression. The present study demonstrated that expression of miR-214 was upregulated in gastric cancer tissues, and positively correlated with lymphatic metastasis and clinical staging. In addition, expression of Dact2 was downregulated in gastric cancer tissues and negatively correlated with lymphatic metastasis and clinical staging. Notably, the present findings suggest that miR-214 promoted the proliferation, migration and invasion of mixed gastric adenocarcinoma type MKN28 cells by suppressing the expression of Dact2.

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Effect of the herbicide atrazine and its metabolite DACT on bovine sperm quality

Cited by 32 publications

References 56 publications

Endocrine‐disrupting chemicals and male reproductive health

Endocrine‐disrupting chemicals and male reproductive health

Effect of environmental contamination on female and male gametes – A lesson from bovines

MicroRNA‑214 promotes the proliferation, migration and invasion of gastric cancer MKN28 cells by suppressing the expression of Dact2

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