Human papillomavirus detection in fine needle aspiration cytology of lymph node metastasis of head and neck squamous cell cancer

Takes, Robert P.; Kaanders, Johannes H.A.M.; Herpen, Carla M.L. van; Merkx, Matthias A.W.; Slootweg, Pieter J.; Melchers, Willem J. G.

doi:10.1016/j.jcv.2016.10.008

Cited by 17 publications

(6 citation statements)

References 25 publications

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“…HPV‐DNA status in FNA was analysed using a highly sensitive genotyping assay that displays an excellent performance on FFPE tissues. Only one previous study used the same primer set of the INNO‐LiPA HPV Genotyping Test to assess HPV‐DNA in FNA . We found no invalid results, and we observed HPV‐positivity in more than 60.0% of the tested specimens.…”

Section: Discussionmentioning

confidence: 47%

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Cytology and direct human papillomavirus testing on fine needle aspirates from cervical lymph node metastases of patients with oropharyngeal squamous cell carcinoma or occult primary

et al. 2018

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Section: Discussionmentioning

confidence: 47%

“…Other studies that used a PCR‐based method found a false‐negative result in 4‐5% of the FNA specimens . However, differently from our study, in which liquid‐based FNA samples were employed, these last studies used cell material scraped from the slide.…”

Section: Discussionmentioning

confidence: 67%

Cytology and direct human papillomavirus testing on fine needle aspirates from cervical lymph node metastases of patients with oropharyngeal squamous cell carcinoma or occult primary

et al. 2018

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“…However, specific data on HPV are lacking in this cohort because most patients were diagnosed by fine needle aspiration (FNA) only and that p16 testing was not widely performed until after 2010. Despite a recent report on the technical feasibility of performing HPV on FNA specimens [23], this was not routine at the time and not done on our specimens.…”

Section: Plos Onementioning

confidence: 95%

Evaluation of radiation treatment volumes for unknown primaries of the head and neck in the era of FDG PET

et al. 2020

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Objectives Positron-emission tomography (PET) has improved identification of the primary tumor as well as occult nodal burden in cancer of the head and neck. Nevertheless, there are still patients where the primary tumor cannot be located. In these situations, the standard of care is comprehensive head and neck radiation therapy however it is unclear whether this is necessary. This study examines the effects of radiation treatment volume on outcomes among using data from two cancer centers in unknown primary carcinoma of the head and neck. Methods Patients received unilateral (n = 34), or bilateral radiation (n = 28). Patient factors such as age, gender, smoking history, and patterns of failure were compared using Mann Whitney U and Chi Square. Overall survival (OS) and disease free survival (DFS) trends were estimated using Kaplan-Meier survival curves. Effect of treatment volume on survival was examined using multivariate cox proportional hazard regression model. Results No significant differences were observed in the frequency of local (p = 0.32), regional (p = 0.50), or distant (p = 0.76) failures between unilateral and bilateral radiation therapy. By Kaplan-Meier estimates, OS (3-year OS bilateral = 71.67%, unilateral = 77.90%, p = 0.50) and DFS (3-year DFS bilateral = 77.92%, unilateral = 69.43%, p = 0.63) were similar between the two treatment approaches. Lastly, multivariate analysis did not demonstrate

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“…A meta-analysis of retrospective studies by Ragin et al [6] indicated that patients with HPV-positive OPSCC exhibited markedly reduced risk of mortality compared to patients affected by HPV-negative OPSCC. Fine needle aspiration cytology (FNAC) with ultrasonography, computed tomography (CT), and MRI are the techniques generally used for the local staging of OPSCC, while positron emission tomography (PET)-CT is useful in locally advanced stages and for carcinomas of unknown primary origin [7][8][9][10][11]. Several studies [11][12][13][14] have reported differences in imaging features of OPSCC by HPV status.…”

Section: Introductionmentioning

confidence: 99%

Multiparametric MRI Evaluation of Oropharyngeal Squamous Cell Carcinoma. A Mono-Institutional Study

Piludu

Marzi

Gangemi

et al. 2021

JCM

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The aim of this paper is to define the pre-treatment radiological characteristics of oropharyngeal squamous cell carcinoma (OPSCC) using morphological and non-morphological magnetic resonance imaging (MRI), based on HPV status, in a single-institution cohort. In total, 100 patients affected by OPSCC were prospectively enrolled in the present study. All patients underwent 1.5T MR with standard sequences, including diffusion-weighted imaging with and intravoxel incoherent motion (IVIM-DWI) technique and a dynamic contrast-enhanced (DCE) MRI. For all patients, human papillomavirus (HPV) status was available. No statistically significant differences in the volume of primary tumors (PTs) and lymph nodes (LNs) were observed based on HPV status. When comparing the two patient groups, no significant differences were found for the PT radiologic characteristics (presence of well-defined borders, exophytic growth, ulceration, and necrosis) and LN morphology (solid/cystic/necrotic). Tumor subsite, smoking status, and alcohol intake significantly differed based on HPV status, as well as ADC and Dt values of both PTs and LNs. We detected no significant difference in DCE-MRI parameters by HPV status. Based on a multivariate logistic regression model, the combination of clinical factors, such as tumor subsite and alcohol habits, with the perfusion-free diffusion coefficient Dt of LNs, may help to accurately discriminate OPSCC by HPV status.

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Human papillomavirus detection in fine needle aspiration cytology of lymph node metastasis of head and neck squamous cell cancer

Abstract: In this study, testing on HPV in FNAC of cervical lymph node metastases of SCC is validated. It provides a valuable alternative for testing of HPV on histological material from patients with oropharyngeal squamous cell carcinoma or cancer of an unknown primary tumor.

Cited by 17 publications

References 25 publications

Cytology and direct human papillomavirus testing on fine needle aspirates from cervical lymph node metastases of patients with oropharyngeal squamous cell carcinoma or occult primary

Cytology and direct human papillomavirus testing on fine needle aspirates from cervical lymph node metastases of patients with oropharyngeal squamous cell carcinoma or occult primary

Evaluation of radiation treatment volumes for unknown primaries of the head and neck in the era of FDG PET

Multiparametric MRI Evaluation of Oropharyngeal Squamous Cell Carcinoma. A Mono-Institutional Study

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