From solvent-free microspheres to bioactive gradient scaffolds

Rasoulianboroujeni, Morteza; Yazdimamaghani, Mostafa; Khoshkenar, Payam; Pothineni, Venkata Raveendra; Kim, Kwang Min; Murray, Teresa A.; Rajadas, Jayakumar; Mills, David K.; Vashaee, Daryoosh; Moharamzadeh, Keyvan; Tayebi, Lobat

doi:10.1016/j.nano.2016.10.008

Cited by 15 publications

(12 citation statements)

References 58 publications

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“…The degree of fusion depends on the sintering temperature and time, varying from the formation of slight new bonding to the pore occlusion and corresponding loss of interconnected porosity when over-sintering conditions are achieved. This method is simple, low cost, highly efficient, and compatible with the manufacturing of scaffolds with complex architectures such as pore size gradients (Figure 4) [48,49]. interconnected porosity when over-sintering conditions are achieved.…”

Section: Heat Sintering Methodsmentioning

confidence: 99%

“…interconnected porosity when over-sintering conditions are achieved. This method is simple, low cost, highly efficient, and compatible with the manufacturing of scaffolds with complex architectures such as pore size gradients (Figure 4) [48,49]. The incorporation of bioactive agents during this scaffold processing method is not trivial, and the thermal stability of the drug of interest must be considered [50], although the use of inorganic compounds such as bioactive glasses [51] is feasible.…”

Section: Heat Sintering Methodsmentioning

confidence: 99%

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Solvent-Free Approaches for the Processing of Scaffolds in Regenerative Medicine

2020

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The regenerative medicine field is seeking novel strategies for the production of synthetic scaffolds that are able to promote the in vivo regeneration of a fully functional tissue. The choices of the scaffold formulation and the manufacturing method are crucial to determine the rate of success of the graft for the intended tissue regeneration process. On one hand, the incorporation of bioactive compounds such as growth factors and drugs in the scaffolds can efficiently guide and promote the spreading, differentiation, growth, and proliferation of cells as well as alleviate post-surgical complications such as foreign body responses and infections. On the other hand, the manufacturing method will determine the feasible morphological properties of the scaffolds and, in certain cases, it can compromise their biocompatibility. In the case of medicated scaffolds, the manufacturing method has also a key effect in the incorporation yield and retained activity of the loaded bioactive agents. In this work, solvent-free methods for scaffolds production, i.e., technological approaches leading to the processing of the porous material with no use of solvents, are presented as advantageous solutions for the processing of medicated scaffolds in terms of efficiency and versatility. The principles of these solvent-free technologies (melt molding, 3D printing by fused deposition modeling, sintering of solid microspheres, gas foaming, and compressed CO2 and supercritical CO2-assisted foaming), a critical discussion of advantages and limitations, as well as selected examples for regenerative medicine purposes are herein presented.

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Section: Heat Sintering Methodsmentioning

confidence: 99%

Section: Heat Sintering Methodsmentioning

confidence: 99%

Solvent-Free Approaches for the Processing of Scaffolds in Regenerative Medicine

2020

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“…The most important part of a scaffold being used for any tissue engineering and regeneration is the design [54][55][56][57][58][59][60][61]. One of the most guaranteed ways to ensure that the design of the scaffold will fit the needs and anatomical design of the affected area is to use clinical imaging data that will define the shape of the anatomical structure in combination with a global and local image database that consists of many different templates for the scaffold design [62,63].…”

Section: Scaffoldsmentioning

confidence: 99%

Cartilage and facial muscle tissue engineering and regeneration: a mini review

et al. 2018

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Cartilage and facial muscle tissue provide basic yet vital functions for homeostasis throughout the body, making human survival and function highly dependent upon these somatic components. When cartilage and facial muscle tissues are harmed or completely destroyed due to disease, trauma, or any other degenerative process, homeostasis and basic body functions consequently become negatively affected. Although most cartilage and cells can regenerate themselves after any form of the aforementioned degenerative disease or trauma, the highly specific characteristics of facial muscles and the specific structures of the cells and tissues required for the proper function cannot be exactly replicated by the body itself. Thus, some form of cartilage and bone tissue engineering is necessary for proper regeneration and function. The use of progenitor cells for this purpose would be very beneficial due to their highly adaptable capabilities, as well as their ability to utilize a high diffusion rate, making them ideal for the specific nature and functions of cartilage and facial muscle tissue. Going along with this, once the progenitor cells are obtained, applying them to a scaffold within the oral cavity in the affected location allows them to adapt to the environment and create cartilage or facial muscle tissue that is specific to the form and function of the area. The principal function of the cartilage and tissue is vascularization, which requires a specific form that allows them to aid the proper flow of bodily functions related to the oral cavity such as oxygen flow and removal of waste. Facial muscle is also very thin, making its reproduction much more possible. Taking all these into consideration, this review aims to highlight and expand upon the primary benefits of the cartilage and facial muscle tissue engineering and regeneration, focusing on how these processes are performed outside of and within the body.

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“…Various techniques are being used for making 3D constructs for specific applications [25][26][27]. Amongst all of the additive manufacturing methods such as stereolithography (SLA), fused deposition modeling (FDM), and selective laser sintering (SLS) are the most advanced ones, however, the pressure-assisted FDM process is one of the most popular for tissue engineering and bioprinting applications [28][29][30][31].…”

Section: Introductionmentioning

confidence: 99%

3D-printed membrane for guided tissue regeneration

Tayebi

Rasoulianboroujeni

Moharamzadeh

et al. 2018

Materials Science and Engineering: C

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Three-dimensional (3D) printing is currently being intensely studied for a diverse set of applications, including the development of bioengineered tissues, as well as the production of functional biomedical materials and devices for dental and orthopedic applications. The aim of this study was to develop and characterize a 3D-printed hybrid construct that can be potentially suitable for guided tissue regeneration (GTR). For this purpose, the rheology analyses have been performed on different bioinks and a specific solution comprising 8% gelatin, 2% elastin and 0.5% sodium hyaluronate has been selected as the most suitable composition for printing a structured membrane for GTR application. Each membrane is composed of 6 layers with strand angles from the first layer to the last layer of 45, 135, 0, 90, 0 and 90°. Confirmed by 3D Laser Measuring imaging, the membrane has small pores on one side and large pores on the other to be able to accommodate different cells like osteoblasts, fibroblasts and keratinocytes on different sides. The ultimate cross-linked product is a 150μm thick flexible and bendable membrane with easy surgical handling. Static and dynamic mechanical testing revealed static tensile modules of 1.95±0.55MPa and a dynamic tensile storage modulus of 314±50kPa. Through seeding the membranes with fibroblast and keratinocyte cells, the results of in vitro tests, including histological analysis, tissue viability examinations and DAPI staining, indicated that the membrane has desirable in vitro biocompatibility. The membrane has demonstrated the barrier function of a GTR membrane by thorough separation of the oral epithelial layer from the underlying tissues. In conclusion, we have characterized a biocompatible and bio-resorbable 3D-printed structured gelatin/elastin/sodium hyaluronate membrane with optimal biostability, mechanical strength and surgical handling characteristics in terms of suturability for potential application in GTR procedures.

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From solvent-free microspheres to bioactive gradient scaffolds

Cited by 15 publications

References 58 publications

Solvent-Free Approaches for the Processing of Scaffolds in Regenerative Medicine

Solvent-Free Approaches for the Processing of Scaffolds in Regenerative Medicine

Cartilage and facial muscle tissue engineering and regeneration: a mini review

3D-printed membrane for guided tissue regeneration

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