miRNA Biogenesis: A Dynamic Pathway

Achkar, Natalia Patricia; Cambiagno, Damián Alejandro; Manavella, Pablo Andrés

doi:10.1016/j.tplants.2016.09.003

Cited by 236 publications

(194 citation statements)

References 84 publications

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“…MiRNA production and function involve a multitude of both general and more specialized factors (Rogers and Chen, 2013;Reis et al, 2015;Achkar et al, 2016). Using a MIM156-based genetic screen, we have identified the F-box protein HWS as a new factor involved in plant miRNA biology.…”

Section: Discussionmentioning

confidence: 99%

“…Mechanisms that control the levels of miRNAs and thereby adjust target silencing have been described: transcriptional regulation ensures the correct spatiotemporal expression of MIR genes, whereas posttranscriptional steps further fine-tune miRNA accumulation (Achkar et al, 2016). Additionally, mature miRISC activity can be attenuated by miRNA target mimicry (Franco-Zorrilla et al, 2007).…”

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confidence: 99%

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A Role for the F-Box Protein HAWAIIAN SKIRT in Plant microRNA Function

et al. 2017

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

A Role for the F-Box Protein HAWAIIAN SKIRT in Plant microRNA Function

et al. 2017

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“…However, the members of HATs and LATs can both be constitutive; without NO 3 -supply, and NO 3 -inducible; stimulated by NO 3 -present in external medium (Miller et al, 2007). Achkar et al 2016 (*see details below), representing miRNA biogenesis machinery and its post-translational regulation. Phosphorylation of miRNA biogenesis cofactors are essential for precise functioning of the pathway in plants.…”

Section: Nitrogen and Diverse Roles Of Mirnasmentioning

confidence: 99%

“…However, substantial efforts are still required to fully understand the dynamics of recruitment, assembly, action, and disassembly of the components of the miRNA machinery. * This figure is adapted from Achkar et al (2016) and reprinted with permission from Elsevier.…”

Section: Nitrogen and Diverse Roles Of Mirnasmentioning

confidence: 99%

Dynamic roles of microRNAs in nutrient acquisition and plant adaptation under nutrient stress: A review

Shahzad¹,

Harlina²,

Ayaad³

et al. 2018

Plant Omics

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A constant supply of soil nutrients is critical for the normal growth and development of plants. However, most environments are unstable and this variability depends on numerous factors that include availability of water content, pH, redox potential, an abundance of organic matter as well as microorganisms in soils. To overcome these hurdles and to maintain nutritional homeostasis, plants have evolved sophisticated systems for the continuous provision of soil nutrients necessary for their uninterrupted growth. In this pressing scenario, plant microRNAs (miRNAs) have emerged as a central regulator of nutrients uptake and transport during limited nutrient conditions. Numerous studies establish the intrinsic involvement of miRNAs and their immediate targets facilitating the core mechanisms related to nutrient homeostasis. In this review, we focus on global overview of miRNAs and their dynamic roles involved in keeping nutritional balance within the plants mediated via post-transcriptional regulation by transcript cleavage or translational inhibition of their target mRNAs. In addition, we have also focused on some of the forefront plant adaptations mediated by miRNAs during nutrient deficiency, such as root architecture modifications, transport channel modulation, long distance signaling and subsequent nutrient mobilization through phloem. Moreover, plant strategy to bring out such alterations is a highly perplexing mechanism that requires changes at large scale which involves coordinated regulation of miRNAs and plant hormones at multiple levels. Deciphering the underlying miRNAs-based mechanisms for streamlining nutrient uptake and transport would be a giant step towards solving this conundrum.

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“…RNA silencing plays key roles in regulating endogenous gene expression, suppressing transposon activity, silencing transgenes, responding to environmental stimuli and combatting viral infection 1,2 . Small RNAs (sRNAs), including miRNA and siRNA, are loaded into an Argonaute (AGO) effector protein to form RNA-induced silencing complexes to repress complementary target RNA at a posttranscriptional gene silencing (PTGS) level through cleavage and/or the inhibition of translation.…”

Section: Introductionmentioning

confidence: 99%

Tomato DCL2b is required for the biosynthesis of 22-nt small RNAs, the resulting secondary siRNAs, and the host defense against ToMV

et al. 2018

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The tomato encode four functional DCL families, of which DCL2 is poorly studied. Here, we generated loss-of-function mutants for a tomato DCL2 gene, dcl2b, and we identified its major role in defending against tomato mosaic virus in relation to both natural and manual infections. Genome-wide small RNA expression profiling revealed that DCL2b was required for the processing 22-nt small RNAs, including a few species of miRNAs. Interestingly, these DCL2b-dependent 22-nt miRNAs functioned similarly to the DCL1-produced 22-nt miRNAs in Arabidopsis and could serve as triggers to generate a class of secondary siRNAs. In particular, the majority of secondary siRNAs were derived from plant defense genes when the plants were challenged with viruses. We also examined differentially expressed genes in dcl2b through RNA-seq and observed that numerous genes were associated with mitochondrial metabolism and hormone signaling under virus-free conditions. Notably, when the loss-of-function dcl2b mutant was challenged with tomato mosaic virus, a group of defense response genes was activated, whereas the genes related to lipid metabolism were suppressed. Together, our findings provided new insights into the roles of tomato DCL2b in small RNA biogenesis and in antiviral defense.

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miRNA Biogenesis: A Dynamic Pathway

Cited by 236 publications

References 84 publications

A Role for the F-Box Protein HAWAIIAN SKIRT in Plant microRNA Function

A Role for the F-Box Protein HAWAIIAN SKIRT in Plant microRNA Function

Dynamic roles of microRNAs in nutrient acquisition and plant adaptation under nutrient stress: A review

Tomato DCL2b is required for the biosynthesis of 22-nt small RNAs, the resulting secondary siRNAs, and the host defense against ToMV

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