Association between dermoscopic and reflectance confocal microscopy features of cutaneous melanoma with <scp>BRAF</scp> mutational status

Bombonato, Caterina; Ribero, Simone; Pozzobon, Flavia Carolina; Puig-Butillé, Joan Antón; Badenas, Cèlia; Carrera, Cristina; Malvehy, Josep; Moscarella, Elvira; Lallas, Aimilios; Piana, Simonetta; Puig, Susana; Argenziano, Giuseppe; Longo, Caterina

doi:10.1111/jdv.14028

Cited by 17 publications

(24 citation statements)

References 22 publications

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“…We found that the majority of dermoscopically similar multiple melanomas showed atypical network or regression structures as the prevalent criterion, in line with our previous findings . Recently, retrospective and prospective studies demonstrated that specific dermoscopic features of melanomas are associated with somatic BRAF mutational pattern, suggesting a potential influence of BRAF alterations on the dermoscopic appearance. However, we could not identify a specific dermoscopic prevalent criterion for concordant mutation‐positive or mutation‐negative similar melanomas, probably due to the low number of concordant cases.…”

Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

“…BRAF mutational status has been associated with dermoscopic appearance of sporadic primary melanoma in a few studies . Two observational retrospective studies showed a correlation between BRAF mutations and the presence of “peppering,” irregular streaks and ulceration . In addition, Armengot‐Carbò et al recently found an association of younger age at diagnosis and dermoscopic blue‐white with BRAF mutation and developed a model based on these two features predicting with good accuracy the occurrence of BRAF mutational status.…”

Section: Introductionmentioning

confidence: 99%

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Dermoscopic similarity is an independent predictor of BRAF mutational concordance in multiple melanomas

Moscarella

Pellegrini

Pampena

et al. 2019

Experimental Dermatology

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Background The association of clinical and dermoscopic features with BRAF mutational status has been poorly analysed in multiple primary melanomas (MPM). Objective To investigate whether concordance of BRAF mutational status is associated with dermoscopic similarity in multiple melanomas of the same patient. Methods Dermoscopic images and corresponding tissue sections of 124 melanomas from 62 patients with MPM were selected at four Italian Dermatology Departments. Similarity of dermoscopic appearance between multiple melanomas was evaluated according to the presence of the same prevalent dermoscopic feature. The BRAFV600 mutational status was analysed with allele‐specific TaqManTM assays or pyrosequencing. Spearman's correlation and univariate and multivariate regression analysis were used for statistical analysis. Results A similar dermoscopic appearance was identified in 38.7% (24/62) of patients with MPM and was correlated with older age at first diagnosis (rho: 0.26; P: 0.042) and occurrence on sun‐damaged skin (rho: 0.27; P: 0.037). The BRAFV600 mutation was detected in 39.5% (49/124) of the tumors and a concordant BRAF mutational status between melanomas in 33/62 (53.2%) MPM patients. Dermoscopically similar melanomas showed 5.7‐fold higher odds to be concordant for BRAF mutational status compared to dissimilar lesions (OR: 5.7; 95% CI 1.7‐19.5; P: 0.005). Conclusion Dermoscopic similarity of multiple melanomas represents an independent clinical predictor of a concordant BRAF mutational status in MPM patients.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dermoscopic similarity is an independent predictor of BRAF mutational concordance in multiple melanomas

Moscarella

Pellegrini

Pampena

et al. 2019

Experimental Dermatology

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“…The absence of meshwork pattern and junctional thickening was identified as independent RCM features predictive of BRAF V600E positivity . Conversely, no association between RCM features and BRAF status was observed in 46 CM investigated by Bombonato et al . Herein, we first evaluated the association of RCM CM subtypes with a specific mutational profile.…”

Section: Discussionmentioning

confidence: 89%

Melanoma types by in vivo reflectance confocal microscopy correlated with protein and molecular genetic alterations: A pilot study

Beretti

Bertoni

Farnetani

et al. 2019

Experimental Dermatology

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Cutaneous melanoma (CM) is one of the most prevalent skin cancers, which lacks both a prognostic marker and a specific and lasting treatment, due to the complexity of the disease and heterogeneity of patients. Reflectance confocal microscopy (RCM) in vivo analysis is a versatile approach offering immediate morphological information, enabling the identification of four primary cutaneous RCM CM types. Whether RCM CM types are associated with a specific protein and molecular genetic profiles at the tissue level remains unclear. The current pilot study was designed to identify potential correlations between RCM CM types and specific biological characteristics, combining immunohistochemistry (IHC) and molecular analyses. Eighty primary CMs evaluated at patient bedside with RCM (type 1 [19, 24%], type 2 [12, 15%], type 3 [7, 9%] and type 4 [42, 52%]) were retrospectively evaluated by IHC stains (CD271, CD20, CD31, cyclin D1), fluorescence in situ hybridization FISH for MYC gain and CDKN2A loss and molecular analysis for somatic mutations (BRAF, NRAS and KIT). RCM CM types correlated with markers of stemness property, density of intra‐tumoral lymphocytic B infiltrate and cyclin D1 expression, while no significant association was found with blood vessel density nor molecular findings. RCM CM types show a different marker profile expression, suggestive of a progression and an increase in aggressiveness, according to RCM morphologies.

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“…Bombonato et al [16] showed that the presence of ulceration and irregular peripheral streaks was positively associated with a BRAF mutated state, while dotted vessels were predictors of wild-type melanomas.…”

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confidence: 99%

Can we Improve Melanoma Detection Methods?

Pampena

Longo

2017

Melanoma Manag.

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Association between dermoscopic and reflectance confocal microscopy features of cutaneous melanoma with BRAF mutational status

Abstract: Ulceration and irregular peripheral streaks represent dermoscopic feature indicative for BRAF-mutated melanoma, while dotted vessels are suggestive for wild-type melanoma.

Cited by 17 publications

References 22 publications

Dermoscopic similarity is an independent predictor of BRAF mutational concordance in multiple melanomas

Dermoscopic similarity is an independent predictor of BRAF mutational concordance in multiple melanomas

Melanoma types by in vivo reflectance confocal microscopy correlated with protein and molecular genetic alterations: A pilot study

Can we Improve Melanoma Detection Methods?

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