Detection and genome characterization of bovine polyomaviruses in beef muscle and ground beef samples from Germany

Gräfe, Donina; Ehlers, Bernhard; Mäde, Dietrich; Ellerbroek, L.; Seidler, Tassilo; Johne, Reimar

doi:10.1016/j.ijfoodmicro.2016.10.024

Cited by 9 publications

(5 citation statements)

References 21 publications

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“…At the end of the 20th century, a number of food scandals and various animal diseases such as bovine spongiform encephalopathy (BSE), avian flu, swine fever, paratuberculosis caused by ruminants, etc opened up a broad public discussion about meat consumption [156] and modern pandemics [157,158]. On the other hand, the hypothesis that the consumption of meat and milk induces chronic inflammation in humans and indirectly increases the risk of colorectal and breast cancer because of Bovine Meat and Milk Factors (BMMF) found predominantly in Eurasian cattle [159][160][161] is less well known. Circular molecules of viral origin, whose genetic material is extrachromosomal present in animals, activate the DNA after the consumption of meat and milk and the transfer into human cells, and are therefore believed to be triggers for the types of cancer mentioned.…”

Section: The Present-day Diet Since the Beginning Of The Industrial Agementioning

confidence: 99%

Nutrition and Health in Human Evolution–Past to Present

2022

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Anyone who wants to understand the biological nature of humans and their special characteristics must look far back into evolutionary history. Today’s way of life is drastically different from that of our ancestors. For almost 99% of human history, gathering and hunting have been the basis of nutrition. It was not until about 12,000 years ago that humans began domesticating plants and animals. Bioarchaeologically and biochemically, this can be traced back to our earliest roots. Modern living conditions and the quality of human life are better today than ever before. However, neither physically nor psychosocially have we made this adjustment and we are paying a high health price for it. The studies presented allow us to reconstruct food supply, lifestyles, and dietary habits: from the earliest primates, through hunter-gatherers of the Paleolithic, farming communities since the beginning of the Anthropocene, to the Industrial Age and the present. The comprehensive data pool allows extraction of all findings of medical relevance. Our recent lifestyle and diet are essentially determined by our culture rather than by our millions of years of ancestry. Culture is permanently in a dominant position compared to natural evolution. Thereby culture does not form a contrast to nature but represents its result. There is no doubt that we are biologically adapted to culture, but it is questionable how much culture humans can cope with.

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Section: The Present-day Diet Since the Beginning Of The Industrial Agementioning

confidence: 99%

Nutrition and Health in Human Evolution–Past to Present

2022

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“…PAdVs have been associated to gastrointestinal disease, diarrhea, and multifactorial respiratory disease in swine (Kumthip et al, 2019). BoPyV was characterized as BoPyV-1, closely related to BoPyV detected in cattle beef muscle and ground beef samples from Germany and USA (Gräfe et al, 2017;Peretti et al, 2015). The areas where BoPyV was detected are involved with educational purposes and dairy productions in a university campus.…”

Section: Discussionmentioning

confidence: 99%

Investigation of Human and Animal Viruses in Water Matrices from a Rural Area in Southeastern Region of Brazil and Their Potential Use as Microbial Source-Tracking Markers

et al. 2023

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This study assessed the sources of contamination of water matrices in a rural area using detection of a host-specific virus (human adenovirus [HAdV], porcine adenovirus [PAdV] and bovine polyomaviruses [BoPyV]) as potential microbial sourcetracking tool, and rotavirus A [RVA], given its epidemiological importance in Brazil. From July 2017 to June 2018, 92 samples were collected from eight points (P1-P8) of surface and raw waters in southeastern region of Brazil. Fifty-five (59.8%) were positive for HAdV, 41 (44.5%) for RVA, 10 (10.9%) for PAdV and four (4.3%) for BoPyV. HAdV and RVA were detected at all sites, and over the entire sampling period, PAdV was detected at a porcine breeding area and at Guarda River site, presenting high concentrations up to 2.6 × 10 9 genome copies per liter [GC/L], and viral concentrations ranging from 9.6 × 10 1 to 7.1 × 10 7 , while BoPyV (1.5 × 10 4 GC/L-9.2 × 10 5 GC/L) was only detected in samples from the bovine breeding areas. The combination of human and animal virus circulation presents a potential impact in the environment due to raw sewage discharge from regional communities, as well as potential hazard to human and animal health.

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“…A study from New Zealand found that infection is more common in bovine fetuses and calves than in adult cattle when batches of bovine serum products are analyzed [14]. Studies based on molecular detection in bovine serum, beef muscle, or ground beef in the USA, Mexico, Germany, and New Zealand found DNA of BoPyV-1, -2, and/or -3 in 2–70% of the tested samples [14,15,19,33,34]. Molecular detection of BoPyV-1 and/or -2 has also been documented in cattle in Spain [35], Belgium [26], and Switzerland [20].…”

Section: Discussionmentioning

confidence: 99%

Bovine polyomavirus-1 (Epsilonpolyomavirus bovis): An emerging fetal pathogen of cattle that causes renal lesions resembling polyomavirus-associated nephropathy of humans

Giannitti

Silveira

Bullock

et al. 2022

Preprint

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Bovine polyomavirus-1 (BoPyV-1, Epsilonpolyomavirus bovis) is widespread in cattle and has been detected in commercialized beef at supermarkets in the USA and Germany. BoPyV-1 has been questioned as a probable zoonotic agent with documented increase in seropositivity in people exposed to cattle. However, to date, BoPyV-1 has not been causally associated with pathology or disease in any animal species, including humans. Here we describe and illustrate pathological findings in an aborted bovine fetus naturally infected with BoPyV-1, providing evidence of its pathogenicity and probable abortigenic potential. Our results indicate that: (i) BoPyV-1 can cause severe kidney lesions in cattle, including tubulointerstitial nephritis with cytopathic changes and necrosis in tubular epithelial cells, tubular and interstitial inflammation, and interstitial fibroplasia; (ii) lesions are at least partly attributable to active viral replication in renal tubular epithelial cells, which have abundant intranuclear viral inclusions; (iii) BoPyV-1 large T (LT) antigen, resulting from early viral gene expression, can be detected in infected renal tubular epithelial cells using a monoclonal antibody raised against Simian Virus-40 polyomavirus LT antigen; and (iv) there is productive BoPyV-1 replication and virion assembly in the nuclei of renal tubular epithelial cells as demonstrated by the ultrastructural observation of abundant arrays of viral particles with typical polyomavirus morphology. Altogether, these lesions resemble the “cytopathic-inflammatory pathology pattern” proposed in the pathogenesis of Human polyomavirus-1-associated nephropathy in immunocompromised people and kidney allograft recipients. Additionally, we sequenced the complete genome of the BoPyV-1 infecting the fetus, which represents the first whole genome of a BoPyV-1 from the Southern Hemisphere. Lastly, the BoPyV-1 strain infecting this fetus was isolated, causing cytopathic effect in Madin-Darby bovine kidney cells. We conclude that BoPyV-1 is pathogenic to the bovine fetus under natural circumstances. Further insights into the epidemiology, biology, clinical relevance, and zoonotic potential of BoPyV-1 are needed.Author SummaryWhile bovine polyomavirus-1 seems to have a broad geographic distribution, whether this virus is responsible for disease and organ damage (lesions) in the cattle it infects is unknown. In this study, we describe and illustrate organ damage in an aborted bovine fetus naturally infected with bovine polyomavirus-1. Our results indicate that this virus is pathogenic, this means it can cause lesions and disease under natural circumstances in cattle. Interestingly, the organ damage in the fetus attributable to this viral infection closely resembles the lesions caused by related viruses within the same viral family (Polyomaviridae) in immunocompromised human patients and kidney transplant recipients. This study sets the bases to frame further research to learn more about whether bovine polyomavirus-1 causes abortion in cattle, and to a broader extent how polyomaviruses cause disease in animals and humans.

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Detection and genome characterization of bovine polyomaviruses in beef muscle and ground beef samples from Germany

Cited by 9 publications

References 21 publications

Nutrition and Health in Human Evolution–Past to Present

Nutrition and Health in Human Evolution–Past to Present

Investigation of Human and Animal Viruses in Water Matrices from a Rural Area in Southeastern Region of Brazil and Their Potential Use as Microbial Source-Tracking Markers

Bovine polyomavirus-1 (Epsilonpolyomavirus bovis): An emerging fetal pathogen of cattle that causes renal lesions resembling polyomavirus-associated nephropathy of humans

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