Autophagy and apoptosis induced by Chinese giant salamander ( Andrias davidianus ) iridovirus (CGSIV)

Du, Jian; Wang, Liqing; Wang, Yuanxian; Shen, Jian; Pan, Chuanyin; Meng, Yan; Yang, Changming; Ji, Hong; Dong, Wuzi

doi:10.1016/j.vetmic.2016.09.011

Cited by 20 publications

(16 citation statements)

References 26 publications

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“…ADRV infection caused a serious systemic hemorrhagic disease and led to more than 90% mortality in Chinese giant salamanders [ 6 , 7 , 9 ]. Although significant progresses have been achieved in understanding the pathogenesis and genome structure of ADRV as well as host immune responses against viral infection [ 9 , 10 , 11 , 12 , 13 , 14 , 15 ], no effective treatment for the virus is available.…”

Section: Introductionmentioning

confidence: 99%

Protective Immunity Induced by DNA Vaccination against Ranavirus Infection in Chinese Giant Salamander Andrias davidianus

Chen

Gao

et al. 2018

Viruses

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Andrias davidianus ranavirus (ADRV) is an emerging viral pathogen that causes severe systemic hemorrhagic disease in Chinese giant salamanders. There is an urgent need for developing an effective vaccine against this fatal disease. In this study, DNA vaccines containing the ADRV 2L gene (pcDNA-2L) and the 58L gene (pcDNA-58L) were respectively constructed, and their immune protective effects were evaluated in Chinese giant salamanders. In vitro and in vivo expression of the vaccine plasmids were confirmed in transfected cells and muscle tissues of vaccinated Chinese giant salamanders by using immunoblot analysis or RT-PCR. Following ADRV challenge, the Chinese giant salamanders vaccinated with pcDNA-2L showed a relative percent survival (RPS) of 66.7%, which was significant higher than that in Chinese giant salamanders immunized with pcDNA-58L (RPS of 3.3%). Moreover, the specific antibody against ADRV was detected in Chinese giant salamanders vaccinated with pcDNA-2L at 14 and 21 days post-vaccination by indirect enzyme-linked immunosorbent assay (ELISA). Transcriptional analysis revealed that the expression levels of immune-related genes including type I interferon (IFN), myxovirus resistance (Mx), major histocompatibility complex class IA (MHC IA), and immunoglobulin M (IgM) were strongly up-regulated after vaccination with pcDNA-2L. Furthermore, vaccination with pcDNA-2L significantly suppressed the virus replication, which was seen by a low viral load in the spleen of Chinese giant salamander survivals after ADRV challenge. These results indicated that pcDNA-2L could induce a significant innate immune response and an adaptive immune response involving both humoral and cell-mediated immunity that conferred effective protection against ADRV infection, and might be a potential vaccine candidate for controlling ADRV disease in Chinese giant salamanders.

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Section: Introductionmentioning

confidence: 99%

Protective Immunity Induced by DNA Vaccination against Ranavirus Infection in Chinese Giant Salamander Andrias davidianus

Chen

Gao

et al. 2018

Viruses

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“…The Chinese giant salamander (CGS, Andrias davidianus ) is the largest extant amphibian in the world [ 24 , 25 ], belonging to the Cryptobranchidae family, which only contains three species: ( Cryptobranchus alleganiensisin in North America, Andrias japonicus in Japan, and Andrias davidianus in China). As an endangered species, the CGS has received increasing attention in evolutionary, comparative biology, and other studies [ 26 - 29 ].…”

Section: Introductionmentioning

confidence: 99%

UCHL1 expression and localization on testicular development and spermatogenesis of Chinese giant salamanders

Wang

Gao

et al. 2017

Oncotarget

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Ubiquitin carboxyl-terminal hydrolase L1 (UCHL1), which is extensively expressed in vertebrates, is a deubiquitinating enzymes that inhibits the degradation of proteins by reversing ubiquitination modification. Herein, a 1087-bp sequence encoding UCHL1 was identified from the Chinese giant salamander (CGS; Andrias davidianus). The coding sequences (CDS) of UCHL1 encoded a putative poly peptide of 222 amino acids. The CGS UCHL1 isoforms were more related to their human and mouse counterparts. The phylogenic tree of vertebrate UCHL1 indicated that CGS UCHL1 has the closest relationship with human UCHL1 (up to 73.99 %). Before the gonads of male CGSs matured, the peak level of UCHL1 expression in testes appeared in 3-year-old CGSs according to RT-qPCR and western blot. In adult testes, the level of UCHL1 protein was lower in the breeding period than in the post-breeding period, whereas the level of UCHL1 protein in interstitial fluid of adult CGS testes was higher during the breeding period than during the post-breeding period. In testicular seminiferous lobules in the developmental stage of CGSs, immunohistochemistry displayed three kinds of localizing patterns of UCHL1, including nuclear localization at half year old, cytoplasmic localization from one year to three years old, and extracellular localization in adult. In testicular seminiferous lobules of adult CGS, the different developmental germ cells were separated by cysts containing UCHL1 protein, but UCHL1 did not localize on the mature sperm. The results showed that extracellular UCHL1 loaded on exosomes, as a component of the homogeneous germ cell cysts, could regulate the synchronous development of sperm in testes of adult CGS.

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“…These models only allowed budding as a mechanism for viral particle production from syncytia, assuming that no apoptosis occurs from fused cells [ 158 ]. Recent reports suggest that this may not be the case [ 159 , 160 , 161 , 162 , 163 , 164 , 165 ], depending on the virus; adjustments during model development and fitting can resolve such issues. In their model, Jacobsen and Pilyugin found that an increase in burst size would allow for tumor control.…”

Section: Modeling Specific Mechanisms Of Actionmentioning

confidence: 99%

Fighting Cancer with Mathematics and Viruses

Santiago

Heidbuechel

Kandell

et al. 2017

Viruses

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After decades of research, oncolytic virotherapy has recently advanced to clinical application, and currently a multitude of novel agents and combination treatments are being evaluated for cancer therapy. Oncolytic agents preferentially replicate in tumor cells, inducing tumor cell lysis and complex antitumor effects, such as innate and adaptive immune responses and the destruction of tumor vasculature. With the availability of different vector platforms and the potential of both genetic engineering and combination regimens to enhance particular aspects of safety and efficacy, the identification of optimal treatments for patient subpopulations or even individual patients becomes a top priority. Mathematical modeling can provide support in this arena by making use of experimental and clinical data to generate hypotheses about the mechanisms underlying complex biology and, ultimately, predict optimal treatment protocols. Increasingly complex models can be applied to account for therapeutically relevant parameters such as components of the immune system. In this review, we describe current developments in oncolytic virotherapy and mathematical modeling to discuss the benefit of integrating different modeling approaches into biological and clinical experimentation. Conclusively, we propose a mutual combination of these research fields to increase the value of the preclinical development and the therapeutic efficacy of the resulting treatments.

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Autophagy and apoptosis induced by Chinese giant salamander ( Andrias davidianus ) iridovirus (CGSIV)

Cited by 20 publications

References 26 publications

Protective Immunity Induced by DNA Vaccination against Ranavirus Infection in Chinese Giant Salamander Andrias davidianus

Protective Immunity Induced by DNA Vaccination against Ranavirus Infection in Chinese Giant Salamander Andrias davidianus

UCHL1 expression and localization on testicular development and spermatogenesis of Chinese giant salamanders

Fighting Cancer with Mathematics and Viruses

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