Correction: Identification of Novel Pre-Erythrocytic Malaria Antigen Candidates for Combination Vaccines with Circumsporozoite Protein

Speake, Cate; Pichugin, Alexander; Sahu, Tejram; Malkov, Vladislav A.; Morrison, Robert; Pei, Ying; Juompan, Laure; Milman, Neta; Zarling, Stasya; Anderson, Charles; Macdonald, Niall P.; Wong-Madden, Sharon; Wendler, Jason P.; Ishizuka, Andrew S.; MacMillen, ZW; Garcia, V; Kappe, SH; Krzych, Urszula; Duffy, Paul

doi:10.1371/journal.pone.0165489

Cited by 2 publications

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“…Using tiling microarrays, Duffy and colleagues identified 124 P. falciparum genes expressed in liver-stage compared to sporozoite or blood-stage. Produced 21 of vaccines with orthologs in P. yoelii and P. berghei, six being protective and two offered improved protection when in combination with CSP, compared to CSP alone (43,44). Many other studies followed these with the purpose of identifying liverstage antigens, to be used as vaccines or drug targets and to understand the pathophysiology of liver-stage malaria and hostpathogen interactions.…”

Section: Antigen Identification Based On Clinical and Challenge Studiesmentioning

confidence: 99%

Current Challenges in the Identification of Pre-Erythrocytic Malaria Vaccine Candidate Antigens

Bettencourt

2020

Front. Immunol.

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Plasmodium spp.-infected mosquitos inject sporozoites into the skin of a mammalian host during a blood meal. These enter the host's circulatory system and establish an infection in the liver. After a silent metamorphosis, merozoites invade the blood leading to the symptomatic and transmissible stages of malaria. The silent pre-erythrocytic malaria stage represents a bottleneck in the disease which is ideal to block progression to clinical malaria, through chemotherapeutic and immunoprophylactic interventions. RTS,S/AS01, the only malaria vaccine close to licensure, although with poor efficacy, blocks the sporozoite invasion mainly through the action of antibodies against the CSP protein, a major component of the pellicle of the sporozoite. Strikingly, sterile protection against malaria can be obtained through immunization with radiation-attenuated sporozoites, genetically attenuated sporozoites or through chemoprophylaxis with infectious sporozoites in animals and humans, but the deployability of sporozoite-based live vaccines pose tremendous challenges. The protection induced by sporozoites occurs in the pre-erythrocytic stages and is mediated mainly by antibodies against the sporozoite and CD8 + T cells against peptides presented by MHC class I molecules in infected hepatocytes. Thus, the identification of malaria antigens expressed in the sporozoite and liver-stage may provide new vaccine candidates to be included, alone or in combination, as recombinant protein-based, virus-like particles or sub-unit virally-vectored vaccines. Here I review the efforts being made to identify Plasmodium falciparum antigens expressed during liver-stage with focus on the development of parasite, hepatocyte, mouse models, and resulting rate of infection in order to identify new vaccine candidates and to improve the efficacy of the current vaccines. Finally, I propose new approaches for the identification of liver-stage antigens based on immunopeptidomics.

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Section: Antigen Identification Based On Clinical and Challenge Studiesmentioning

confidence: 99%

Current Challenges in the Identification of Pre-Erythrocytic Malaria Vaccine Candidate Antigens

Bettencourt

2020

Front. Immunol.

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Contribution of Transcriptome to Elucidate the Biology of Plasmodium spp

Parreira¹,

Scarpelli

Lima³

et al. 2022

CTMC

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In the present review, we discuss some of the new technologies that have been applied to elucidate how Plasmodium spp escape from the immune system and subvert the host physiology to orchestrate the regulation of its biological pathways. Our manuscript describes how techniques such as microarray approaches, RNA-Seq and single-cell RNA sequencing have contributed to the discovery of transcripts and changed the concept of gene expression regulation in closely related malaria parasite species. Moreover, the text highlights the contributions of high-throughput RNA sequencing for the current knowledge of malaria parasite biology, physiology, vaccine target and the revelation of new players in parasite signaling.

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Correction: Identification of Novel Pre-Erythrocytic Malaria Antigen Candidates for Combination Vaccines with Circumsporozoite Protein

Cited by 2 publications

References 1 publication

Current Challenges in the Identification of Pre-Erythrocytic Malaria Vaccine Candidate Antigens

Current Challenges in the Identification of Pre-Erythrocytic Malaria Vaccine Candidate Antigens

Contribution of Transcriptome to Elucidate the Biology of Plasmodium spp

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