Reinforcement of the Flexor Tendon Repair Using Human Amniotic Membrane

Doğramacı, Yunus; Duman, İbrahim

doi:10.7547/15-036

Cited by 7 publications

(2 citation statements)

References 23 publications

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“…An alternative to amnion-derived cells, human amniotic membrane (HAM) is a more conveniently obtainable source which has been used in burn patients and to prevent peritendinous adhesions 45 . Dogramaci et al have recently demonstrated favorable results following application of HAM in an ovine flexor tendon reinforced tendon repair.…”

Section: Current Cell-based Approachesmentioning

confidence: 99%

Cell-based Approaches for Augmentation of Tendon Repair

Carballo

Lebaschi

Rodeo

2017

Techniques in Shoulder &Amp; Elbow Surgery

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Cell-based approaches are among the principal interventions in orthobiologics to improve tendon and ligament healing and to combat degenerative processes. The number of options available for investigation are expanding rapidly and investigators have an increasing number of cell types to choose from for research purposes. However, in part due to the current regulatory environment, the list of available cells at clinicians’ disposal for therapeutic purposes is still rather limited. In this review, we present an overview of the main cellular categories in current use. Notable recent developments in cell-based approaches include the introduction of diverse sources of mesenchymal stem cells, pluripotent cells of extra-embryonic origin, and the emerging popularity of fully differentiated cells such as tenocytes and endothelial cells. Delivery strategies are discussed and a succinct discussion of the current regulatory environment in the United States is presented.

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Section: Current Cell-based Approachesmentioning

confidence: 99%

Cell-based Approaches for Augmentation of Tendon Repair

Carballo

Lebaschi

Rodeo

2017

Techniques in Shoulder &Amp; Elbow Surgery

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“…23 In its early use as a intact membrane sheet, the AM has been suggested to reduce scar formation following digital flexor tendon repair. 24 In more-recent tendon repair studies, the amniotic membrane has been used as a two-dimensional support in animal models of tendon repair, 25,26 highlighting the membrane’s ability to enhance the mechanical properties of the repair without addressing its potential role in the inflammatory process.…”

Section: Introductionmentioning

confidence: 99%

Incorporation of the Amniotic Membrane as an Immunomodulatory Design Element in Collagen Scaffolds for Tendon Repair

Hortensius

Ebens

Dewey

et al. 2018

ACS Biomater. Sci. Eng.

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Tendon injuries often require surgical intervention and even then result in poor outcomes due to scar formation and repeated failure. Biomaterial implants offer the potential to address multiple underlying concerns preventing improved tendon repair. Here, we describe modifications to the composition of an anisotropic collagen–glycosaminoglycan (CG) scaffold biomaterial, incorporating amniotic membrane (AM)-derived matrix to alter the inflammatory response and establish conditions for improved regenerative repair. We explored two methods of AM matrix incorporation to address multiple concerns associated with tendon repair. Amniotic membrane-derived matrix was incorporated directly into the scaffold microstructure during fabrication to form a C/AM composite. Alternatively, decellularized amniotic matrix was wrapped around the traditional collagen–chondroitin sulfate (C/CS) scaffold to form a core–shell composite (C/CS plus AM wrap) in a manner similar to current collagen membrane wraps used in rotator cuff and Achilles tendon surgeries to improve the mechanical strength of the repair. Human mesenchymal stem cells (MSCs) cultured within these materials were evaluated for metabolic health and immunomodulatory gene expression in response to inflammatory media challenge of interleukin 1 β and tumor necrosis factor α. The scaffolds were able to maintain MSC metabolic activity in all media conditions over the course of a 7 day culture. Expression of genes encoding for pro-inflammatory cytokines were down-regulated in AM containing scaffolds, suggesting the potential to employ AM-modified CG scaffolds for tendon-repair applications.

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