Bacterial lipid diversity

López‐Lara, Isabel M.; Geiger, Otto

doi:10.1016/j.bbalip.2016.10.007

Cited by 109 publications

(98 citation statements)

References 128 publications

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“…PE-NMe have been estimated to occur in only about 10-15% of bacteria 32,43 , and in our analysis they were strongly correlated to Lautropia , a genus not previously described as synthesizing PE- NMe ( Figure 6a-b ). To validate this potentially novel finding, we compared lipid levels from two in vitro grown strains of Lautropia mirabilis to Actinomyces odontolyticus.…”

Section: Resultssupporting

confidence: 61%

“…We argue that MS-based ‘omics technologies could further strengthen our understanding of the host-microbe interactions, not only in being able to monitor potential protein response to microbial invasion, but also in monitoring lipid changes in these host-microbe environments. Lipids are important biomolecules related to defense and invasion as they reside at the interface between cells and serve as structural or signaling molecules 43 . In support of this idea, we found that the same host-derived proteins associated with PD were also strongly correlated with lipids classified as PCs or plasmenyl-PCs.…”

Section: Discussionmentioning

confidence: 99%

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Proteomics, lipidomics, metabolomics and 16S DNA sequencing of dental plaque from patients with diabetes and periodontal disease

Overmyer

Rhoads

Merrill

et al. 2020

Preprint

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21Oral microbiome influences human health, specifically pre-and type 2 diabetes (Pre-DM/DM) and 22 periodontal diseases (PD), through complex microbial interactions. To explore these relations, we 23 performed 16S rDNA sequencing, metabolomics, lipidomics, and proteomics analyses on 24 supragingival dental plaque collected from individuals with Pre-DM/DM (n=39), Pre-DM/DM and 25 PD (n=37), PD alone (n=11), or neither (n=10). We identified on average 2,790 operational 26 taxonomic units and 2,025 microbial and host proteins per sample and quantified 110 metabolites 27 and 415 lipids. Plaque samples from Pre-DM/DM patients contained higher abundance of 28 Fusobacterium and Tannerella vs. plaques from metabolically healthy. Phosphatidylcholines, 29 plasmenyl-phosphatidylcholines, ceramides containing non-OH fatty acids, and host proteins 30 related to actin filament rearrangement were elevated in plaques from PD vs. non-PD. Cross-31 omic correlation analysis enabled the detection of a strong association between Lautropia and 32 mono-methyl phophospotidlyethanolamine (PE-NMe), striking because synthesis of PE-NMe is 33 uncommon in oral bacteria. Lipidomics analysis of in vitro cultures of Lautropia mirabilis confirmed 34 the bacteria's synthesis of PE-NMe. This comprehensive analysis revealed a novel microbial 35 metabolic pathway and significant associations of host-derived proteins with PD. 36 42 contribute to the progression of PD are also correlated with systemic diseases, including diabetes, 43arthritis, and heart disease 5-7 , suggesting that oral microbial ecologies have a broad impact on 44 human health, and a better understanding of pathogenesis and host-microbe interactions will be 45 essential for mitigating negative effects of pathogenic microbiota. 46With poor oral hygiene, bacterial populations accumulate, become increasingly diverse, and 47

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Section: Resultssupporting

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Proteomics, lipidomics, metabolomics and 16S DNA sequencing of dental plaque from patients with diabetes and periodontal disease

Overmyer

Rhoads

Merrill

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…To test this hypothesis, we used a range of phospholipids in addition to PG and PE. These included phosphatidylcholine (PC), a relative uncommon phospholipid in bacteria and absent in the aforementioned marine bacteria, phosphatidic acid (PA), an intermediate in the biosynthesis of phospholipids, and phosphatidylserine (PS) and phosphatidylinositol (PI), both of which are associated primarily with eukaryotes (17,18). To rule out any potential effect of fatty acid chain length on PlcP activity across different phospholipid species, all lipid substrates contained sn-1 C 16:0 /sn-2 C 16:0 palmitic acid.…”

Section: Resultsmentioning

confidence: 99%

Manganese Is Essential for PlcP Metallophosphoesterase Activity Involved in Lipid Remodeling in Abundant Marine Heterotrophic Bacteria

Wei

Quareshy

Zhang

et al. 2018

Appl Environ Microbiol

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In vast areas of the ocean, microbes must adapt to the availability of scarce nutrients, and a key strategy for reducing the cellular phosphorus (P) quota is to remodel membranes by replacing phospholipids with non-P surrogate lipids. A metallophosphoesterase, PlcP, is essential for lipid remodeling in cosmopolitan marine bacteria of the (e.g., sp. strain MED193) and SAR11 (e.g., sp. strain HTCC7211) clades, and transcription of is known to be induced by P limitation. In order to better understand PlcP-mediated lipid remodeling, we sought to characterize PlcP for its metal ion requirement and to determine its selectivity for native bacterial phospholipids. Here, we report the occurrence of a highly conserved binuclear ion center in PlcPs from MED193 and HTCC7211 and show that manganese is the preferred metal for metallophosphoesterase activity. PlcP displayed high activity towards the major bacterial phospholipids, e.g., phosphatidylglycerol but also phosphatidic acid, a key intermediate in phospholipid biosynthesis. In contrast, phosphatidylserine and phosphatidylinositol, both of which are rare lipids in bacteria, are not preferred substrates. These data suggest that PlcP undertakes a generic lipid remodeling role during the cellular response of marine bacteria to P deficiency and that manganese availability may play a key role in regulating the lipid remodeling process. Membrane lipids form the structural basis of all cells. In the marine environment, it is well established that phosphorus availability significantly affects lipid composition in cosmopolitan marine bacteria, whereby non-phosphorus-containing lipids are used to replace phospholipids in response to phosphorus stress. Central to this lipid remodeling pathway is a newly identified phospholipase C-type metallophosphoesterase (PlcP). However, little is known about how PlcP activity is regulated. Here, we determined the role of metal ions in regulating PlcP activity and compared PlcP substrate specificities in PlcP enzymes from two model marine bacteria from the marine clade and the SAR11 clade. Our data provide new insights into the regulation of lipid remodeling in these marine bacteria.

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“…Downstream samples collected from Bison Pool, Mound Spring, and Empress Pool featured many DAG phospholipids common to unspecific Bacteria and Eukaryotes (Figure 4, yellow bars 25-31), such as DPG, PC, PE, PG, PME, and PS (Kent, 1995;López-Lara and Geiger, 2017). These lipids were not abundant in Octopus Spring sample OS2, possibly because the temperature measured at this sample site, 59.8 • C, roughly corresponds to the upper temperature limit of about 60 • C reported for Eukaryotes (Tansey and Brock, 1972).…”

Section: Ipl Headgroup and Backbone Distributionsmentioning

confidence: 99%

Carbon Oxidation State in Microbial Polar Lipids Suggests Adaptation to Hot Spring Temperature and Redox Gradients

et al. 2020

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The influence of oxidation-reduction (redox) potential on the expression of biomolecules is a topic of ongoing exploration in geobiology. In this study, we investigate the novel possibility that structures and compositions of lipids produced by microbial communities are sensitive to environmental redox conditions. We extracted lipids from microbial biomass collected along the thermal and redox gradients of four alkaline hot springs in Yellowstone National Park (YNP) and investigated patterns in the average oxidation state of carbon (Z C ), a metric calculated from the chemical formulae of lipid structures. Carbon in intact polar lipids (IPLs) and their alkyl chains becomes more oxidized (higher Z C ) with increasing distance from each of the four hot spring sources. This coincides with decreased water temperature and increased concentrations of oxidized inorganic solutes, such as dissolved oxygen, sulfate, and nitrate. Carbon in IPLs is most reduced (lowest Z C ) in the hot, reduced conditions upstream, with abundance-weighted Z C values between −1.68 and −1.56. These values increase gradually downstream to around −1.36 to −1.33 in microbial communities living between 29.0 and 38.1 • C. This near-linear increase in Z C can be attributed to a shift from ether-linked to ester-linked alkyl chains, a decrease in average aliphatic carbons per chain (nC), an increase in average degree of unsaturation per chain (nUnsat), and increased cyclization in tetraether lipids. The Z C of lipid headgroups and backbones did not change significantly downstream. Expression of lipids with relatively reduced carbon under reduced conditions and oxidized lipids under oxidized conditions may indicate microbial adaptation across environmental gradients in temperature and electron donor/acceptor supply.

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Bacterial lipid diversity

Cited by 109 publications

References 128 publications

Proteomics, lipidomics, metabolomics and 16S DNA sequencing of dental plaque from patients with diabetes and periodontal disease

Proteomics, lipidomics, metabolomics and 16S DNA sequencing of dental plaque from patients with diabetes and periodontal disease

Manganese Is Essential for PlcP Metallophosphoesterase Activity Involved in Lipid Remodeling in Abundant Marine Heterotrophic Bacteria

Carbon Oxidation State in Microbial Polar Lipids Suggests Adaptation to Hot Spring Temperature and Redox Gradients

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