Multi-locus DNA barcoding identifies matK as a suitable marker for species identification in Hibiscus L.

Abstract

The genus Hibiscus L. includes several taxa of medicinal value and species used for the extraction of natural dyes. These applications require the use of authentic plant materials. DNA barcoding is a molecular method for species identification, which helps in reliable authentication by using one or more DNA barcode marker. In this study, we have collected 44 accessions, representing 16 species of Hibiscus, distributed in the southern peninsular India, to evaluate the discriminatory power of the two core barcodes rbcLa and matK together with the suggested additional regions trnH-psbA and ITS2. No intraspecies divergence was observed among the accessions studied. Interspecies divergence was 0%-9.6% with individual markers, which increased to 0%-12.5% and 0.8%-20.3% when using two- and three-marker combinations, respectively. Differentiation of all the species of Hibiscus was possible with the matK DNA barcode marker. Also, in two-marker combinations, only those combinations with matK differentiated all the species. Though all the three-marker combinations showed 100% species differentiation, species resolution was consistently better when the matK marker formed part of the combination. These results clearly showed that matK is more suitable when compared to rbcLa, trnH-psbA, and ITS2 for species identification in Hibiscus.

Does Matk, Trnh-Psba, Trnl-F And Its1 Identification Mirror Morphological Identification In Hibisceae?

“…The BLAST analysis based on the matK locus showed incorrect identification of H. sabdariffa and ambiguous identification of both H. rosa-sinensis and M. arboreus (Table 03). These findings are contradictory to those reported by Poovitha et al (2016) who found that matK was a suitable barcode for Hibiscus including H. rosa-sinensis and H. sabdariffa. Hence, the purity of the species or the evolution of the species at different localities may influence the effectiveness of DNA barcoding to identify species.…”

“…The success of species identification using DNA barcoding depends very much on the taxa in question, as much as the utilised marker (Amandita et al, 2019). For example, in Hibiscus, including H. rosa-sinensis and H. sabdariffa, matK was found to be the most suitable when compared to rbcLa, trnH-psbA and ITS2 (Poovitha et al, 2016). However, to discriminate Hibiscus syriacus from its adulterants correctly, ITS2 was recommended (Liu et al, 2014).…”

“…Loci such as atpB, rbcL, and ndhF, have focused on phylogeny at higher taxonomic levels (Pfeil et al, 2002;Pfeil & Crisp, 2005;Tate & Simpson, 2003). Only two studies specifically on Hibisceae and DNA barcoding were found in November of 2019, that is, the studies of Poovitha et al, (2016) and Liu et al (2014).…”

Protein extracts from Momordica charantia var. charantia and M. charantia var. muricata show anti‐lipidemic and antioxidant properties in experimental type 2 diabetic rats

“…For DNA barcoding, total genomic DNA was isolated as per the method described by Saghai‐Maroof, Soliman, Jorgensen, and Allard (1984). DNA barcoding was carried out using ITS2, trnH‐psbA, rbc L, and mat K markers as described before (Poovitha, Nithaniyal, Balaji, & Parani, 2016).…”

The complete chloroplast genome of Ocimum gratissimum from India – a medicinal plant in the Lamiaceae

“…The herbarium voucher specimen (SRMH000142) was deposited in the SRM IST Herbarium at the SRM Institute of Science and Technology, Kattankulathur, India (Herbarium curator: Dr. E. Pandian, Email: pandiane@ srmist.edu.in). Total genomic DNA from O. gratissimum was extracted using the CTAB method (Doyle and Doyle 1987), with some modifications (Poovitha et al 2016). A whole-genome DNA sequencing library was constructed using the Nextera XT Library Prep Kit.…”