Cytotoxicity, haemolymphatic parameters, and oxidative stress following exposure to sub-lethal concentrations of quaternium-15 in Mytilus galloprovincialis

Faggio, Caterina; Pagano, Maria; Alampi, Roberto; Vazzana, Irene; Felice, Maria Rosa

doi:10.1016/j.aquatox.2016.10.010

Cited by 191 publications

(49 citation statements)

References 72 publications

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“…Marine bivalve molluscs have complex systems to defence against infection which include humoral factors and cellular mechanism (Faggio, Pagano, Alampi, Vazzana, & Felice, ; Faggio, Tsarpali, & Dailianis, ; Sehonova, Svobodova, Dolezelova, Vosmerova, & Faggio, ). Among the humoral factors involved in the elimination of pathogens, molecules such as antimicrobial peptides, major plasma proteins, lysosomal enzymes, lectins and protease inhibitors can be considered (Matozzo, Pagano, Spinelli, Caicci, & Faggio, ; Soudant et al, ; Tiscar & Mosca, ).…”

Section: Discussionmentioning

confidence: 99%

Perkinsus mediterraneus infection induces oxidative stress in the mollusc Mimachlamys varia

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Capó

Tejada

et al. 2019

Journal of Fish Diseases

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Perkinsus mediterraneus is a protozoan parasite that can cause marine mollusc diseases known as perkinsosis being a serious threat for clam cultures worldwide. The aim of the present study was first to determine the Perkinsus species infecting the variegated scallop Mimachlamys varia and then to evaluate the existence of oxidative stress in gills of M. varia according to different degrees of infection. DNA sequencing confirmed that P. mediterraneus was the species infecting M. varia. ROS production was progressively increasing with the degree of infection although the differences were only significant in the high‐infected group. Low degree of infection significantly increased superoxide dismutase (SOD) and glutathione S‐transferase (GST) activities and nitrite levels with respect to the control group. In the high‐infected group, a significant increase was evidenced in all analysed enzymes, catalase, SOD, glutathione reductase and GST. Non‐significant differences in MDA levels were observed between the control and low‐infected groups; however, a significant increase in MDA levels was observed in the high‐infected group. In conclusion, the infection by Perkinsus mediterraneus in M. varia induces oxidative stress and an antioxidant response directly related to the infection degree that can contribute to the pathogenicity of the infection.

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Section: Discussionmentioning

confidence: 99%

Perkinsus mediterraneus infection induces oxidative stress in the mollusc Mimachlamys varia

Box

Capó

Tejada

et al. 2019

Journal of Fish Diseases

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“…The aquatic environment is the ultimate recipient of an increasing amount of contaminants as a result of the discharge of industrial, agricultural, and urban wastes (Bartoskova et al, ; Capillo et al, ; Faggio, Pagano, Alampi, Vazzana, & Felice, ; Sehonova, Svobodova, Dolezelova, Vosmerova, & Faggio, ). A large number of pollutants in this complex mixture are responsible for multiple effects in organisms, including human beings, and ecosystem levels, affecting organ function, reproductive status, species survival, population size, and ultimately biodiversity (Aliko, Qirjo, Sula, Morina, & Faggio, ; Bickham, Sandhu, Hebert, Chikhi, & Athwal, ; Fazio et al, ; Fiorino et al, ; Pagano et al, ).…”

Section: Introductionmentioning

confidence: 99%

Digital light microscopy as a tool in toxicological evaluation of fish erythrocyte morphological abnormalities

Sula

Aliko

Pagano

et al. 2019

Microscopy Res & Technique

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Fish erythrocytes are very sensitive to environmental pollution because they exhibit pathological alterations before appearance of other external symptoms, may be used as reliable toxicity bioindicators. Microscopic analysis can be a useful tool in early detection of alterations in erythrocyte morphology due to different environmental stressors, and in predicting organism's possible response ways. This study aims to evaluate the toxicity of a polluted freshwater lake on the erythrocytes of crucian carp fish, Carassius carassius, using digital light microscopy (DLM). Scanning DLM micrographs revealed the presence of nuclear and cytoplasmic abnormalities like micronucleus, nuclear buds, lobed nucleus, deformed nucleus, vacuolated cytoplasm, and echinocyte in the erythrocytes of crucian carp fish from a polluted lake. The obtained results substantiate that computation of erythrocyte morphological damages as biomarkers of exposure, through digital light microscopy software, can be effectively used as a toxicological tool.

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“…The digestive cell type exhibits a well-established endo-lysosomal vacuolar system, such as heterolysosomes, heterophagosomes, and residual bodies, which takes part during the process of intra-cellular digestion (Dimitriadis et al, 2004). Additionally, the lysosomes of digestive cells are not only involved in the intra-cellular digestion of nutrients and antioxidant defenses (Faggio et al, 2016), but also regarded as an important organelle of organic pollutant and metal sequestration and detoxification (Marigómez et al, 2002;Pagano et al, 2016). Besides, the gills of filter-feeding bivalves are generally considered one of the major target organs for accumulating waterborne pollutants, either dissolved in or bound to particulate matter.…”

Section: Discussionmentioning

confidence: 99%

Novel ABCB1 and ABCC Transporters Are Involved in the Detoxification of Benzo(α)pyrene in Thick Shell Mussel, Mytilus coruscus

Guo

Yan

et al. 2020

Front. Mar. Sci.

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ATP-binding cassette (ABC) transporters play an essential role in xenobiotic resistance through transporting xenobiotics into or out of cells. However, their functional role in mollusk's defense against polycyclic aromatic hydrocarbons (PAHs) remains unclear. In the present study, novel McoABCB1 and McoABCC transporters were identified in the gills of Mytilus coruscus, and their benzo(α)pyrene (Bap) detoxification function was determined by qRT-PCR and efflux assays. McoABCB1 and McoABCC genes were constitutively expressed in all tissue types, suggesting the primary physiological function of these transporters. Bap, a chemical stress agent, could induce the mRNA expression of McoABCB1 and McoABCC in gills. However, at high concentration, Bap inhibited their expression levels at the late stage. Besides, the efflux activities in M. coruscus gills showed a dose-dependent elevation in a Bap pre-exposure group, compared with those in a reversin 205 or MK571 treatment alone group. These results demonstrated the presence of ABCB1 and ABCC transporters in Mytilus coruscus, and their involvement in cellular Bap detoxification.

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Cytotoxicity, haemolymphatic parameters, and oxidative stress following exposure to sub-lethal concentrations of quaternium-15 in Mytilus galloprovincialis

Cited by 191 publications

References 72 publications

Perkinsus mediterraneus infection induces oxidative stress in the mollusc Mimachlamys varia

Perkinsus mediterraneus infection induces oxidative stress in the mollusc Mimachlamys varia

Digital light microscopy as a tool in toxicological evaluation of fish erythrocyte morphological abnormalities

Novel ABCB1 and ABCC Transporters Are Involved in the Detoxification of Benzo(α)pyrene in Thick Shell Mussel, Mytilus coruscus

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