The In Vitro Effectiveness of Lactobacillus fermentum Against Different Candida Species Compared With Broadly Used Azoles

Deidda, Francesca; Amoruso, Angela; Nicola, Stefania; Graziano, Teresa; Pane, Marco; Allesina, Serena; Raiteri, Elisabetta; Piano, M. Del; Mogna, Luca

doi:10.1097/mcg.0000000000000686

Cited by 11 publications

(9 citation statements)

References 15 publications

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“…This probiotic formulation is ad hoc designed, patented, and produced by Probiotical SPA-Novara, Italy. The choice of the strains was done considering the results of previous studies that showed their capabilities to counteract gut pathogens, such as some Candida strains [15], Enterobacteria [16,17], their anti-inflammatory properties and Fig. 1 Study flowchart for baseline analysis and phase 1 trial.…”

Section: Probiotic Supplementmentioning

confidence: 99%

“…1 Study flowchart for baseline analysis and phase 1 trial. ALS, amyotrophic lateral sclerosis; T0, baseline; T1, 3 months from baseline; T2, 6 months from baseline; ALSFRS-R, ALS Functional Rating Scale-Revised; BMI, body mass index; FVC%, force vital capacity %; DGGE, denaturing gradient gel electrophoresis; qPCR, quantitative polymerase chain reaction; NGS, next-generation sequencing positive influence in restoring the gut physiological barrier [15,18].…”

Section: Probiotic Supplementmentioning

confidence: 99%

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A prospective longitudinal study on the microbiota composition in amyotrophic lateral sclerosis

Gioia

Cionci

Baffoni

et al. 2020

BMC Med

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Background A connection between amyotrophic lateral sclerosis (ALS) and altered gut microbiota composition has previously been reported in animal models. This work is the first prospective longitudinal study addressing the microbiota composition in ALS patients and the impact of a probiotic supplementation on the gut microbiota and disease progression. Methods Fifty patients and 50 matched controls were enrolled. The microbial profile of stool samples from patients and controls was analyzed via PCR-Denaturing Gradient Gel Electrophoresis, and the main microbial groups quantified via qPCR. The whole microbiota was then analyzed via next generation sequencing after amplification of the V3–V4 region of 16S rDNA. Patients were then randomized to receive probiotic treatment or placebo and followed up for 6 months with ALSFRS-R, BMI, and FVC%. Results The results demonstrate that the gut microbiota of ALS patients is characterized by some differences with respect to controls, regardless of the disability degree. Moreover, the gut microbiota composition changes during the course of the disease as demonstrated by the significant decrease in the number of observed operational taxonomic unit during the follow-up. Interestingly, an unbalance between potentially protective microbial groups, such as Bacteroidetes, and other with potential neurotoxic or pro-inflammatory activity, such as Cyanobacteria, has been shown. The 6-month probiotic treatment influenced the gut microbial composition; however, it did not bring the biodiversity of intestinal microbiota of patients closer to that of control subjects and no influence on the progression of the disease measured by ALSFRS-R was demonstrated. Conclusions Our study poses the bases for larger clinical studies to characterize the microbiota changes as a novel ALS biomarker and to test new microbial strategy to ameliorate the health status of the gut. Trial registration CE 107/14, approved by the Ethics Committee of the “Maggiore della Carità” University Hospital, Italy.

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Section: Probiotic Supplementmentioning

confidence: 99%

Section: Probiotic Supplementmentioning

confidence: 99%

A prospective longitudinal study on the microbiota composition in amyotrophic lateral sclerosis

Gioia

Cionci

Baffoni

et al. 2020

BMC Med

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“…In the female genital tract, a healthy vaginal status is commonly associated with low microbial diversity and prevalence of Lactobacillus. Commensal vaginal Lactobacillus species are thought to defend against many pathogens, such as Candida infection [7,8], sexually transmitted diseases [9], urinary tract infections [10,11] and human immunode ciency virus (HIV) infection [12]. Bacterial vaginosis (BV) is associated with a higher risk of miscarriage, preterm premature rupture of membranes, HPV infection and sexually transmitted infections [13,14,15].…”

Section: Introductionmentioning

confidence: 99%

Human papillomavirus infection and cervical intraepithelial neoplasia progression are associated with increased vaginal microbiome diversity in a Chinese cohort

Chen

Qiu

Wang

et al. 2020

Preprint

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Background: In this study, the association between human papillomavirus (HPV) infection and related cervical intraepithelial neoplasia (CIN) or cervical cancer and vaginal microbiome was evaluated in Chinese cohorts. Methods: The vaginal bacterial composition of five groups, HPV-infected women without CINs (HPV, n=78), women with low-grade squamous intraepithelial lesions (LSIL, n=51), women with high-grade squamous intraepithelial lesions (HSIL, n=23), women with invasive cervical cancer (Cancer, n=9) and healthy women without HPV infection (Normal, n=68), was characterized by deep sequencing of barcoded 16S rRNA gene fragments (V3-4) using Illumina MiSeq. Results: HPV infection increased vaginal bacterial richness and diversity regardless of the status of CINs. The vaginal bacterial richness and diversity were further augmented in women with cervical cancer. Lactobacillus was the most abundant genus in all groups. HPV infection had a negative influence on the abundances of Lactobacillus, Gardnerella and Atopobium. Accordingly, HPV infection increased the relative abundance of Prevotella, Bacillus, Anaerococcus, Sneathia, Megasphaera, Streptococcus and Anaerococcus. The increased proportions of Bacillus, Anaerococcus and the reduced abundance of Gradnerella vaginalis were probably related with the progression of CINs severity. HPV infection without CINs or cancerous lesions was strongly associated with Megasphaera. The most abundant bacterium in the LSIL group was Prevotella amnii. However, Prevotella timonensis, Shuttleworthia and Streptococcaceae at the family level were three taxa related to HSIL. Furthermore, more taxa were associated with the Cancer group including Bacillus, Sneathia, Acidovorax, Oceanobacillus profundus, Fusobacterium, Veillonellaceae at the family level, Anaerococcus and Porphyromonas uenonis. Samples in the Normal group were mostly assigned to CST III. HPV infection converted the vaginal bacterial community structure from CST III to CST IV. Furthermore, the proportions of CST IV were gradually augmented with the progression of the severity of CINs.Conclusions: This work interpreted the differential vaginal bacteria under HPV infection and various precancerous or cancerous lesions in a Chinese cohort. We distinguished the specific microbes and the vaginal bacterial structure that were related with the progression of CINs severity in Chinese women.

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“…A large number of microorganisms inhabit the female genital tract, and hundreds of studies have revealed that a dominance of Lactobacillus lowers bacterial richness and diversity, indicating a healthy vaginal status. A healthy microbial biofilm may prevent or hinder many urogenital diseases, such as Candida infection [5,6], sexually transmitted diseases [7], urinary tract infections [8,9] and human immunodeficiency virus (HIV) infection [10]. Some studies to date have also reported that changes in the vaginal microbial structure have a close connection with HPV infection [11][12][13][14] and CIN progression [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Association between the vaginal microbiome and high-risk human papillomavirus infection in pregnant Chinese women

et al. 2019

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Background: In this study, the association between high-risk human papillomavirus (hrHPV) infection and the vaginal microbiome in pregnant women was evaluated in Chinese cohorts. Methods: The vaginal bacterial composition of four groups, 38 hrHPV-infected pregnant women (PHR, n = 38), pregnant women without HPV infection (PN, n = 48), nonpregnant women with hrHPV infection (NPHR, n = 19) and nonpregnant women without HPV infection (NPN, n = 30), was characterized by deep sequencing of barcoded 16S rRNA gene fragments (V3-4) using Illumina MiSeq. Results: The results revealed that both pregnancy and HPV infection can increase vaginal bacterial microbial richness and diversity, with the bacterial composition being most influenced by pregnancy. Lactobacillus was the most dominant genus among all samples. NPN samples were dominated by CST (community state type) III, mainly composed of Lactobacillus iners. Both pregnancy and hrHPV infection were accompanied by an increased proportion of CST I (dominated by Lactobacillus crispatus), as opposed to CST III. Bifidobacterium, Bacillus, Megasphaera, Sneathia, Prevotella, Gardnerella, Fastidiosipila and Dialister were found to be biomarkers for hrHPV-infected women, though different genera (Bifidobacterium, Megasphaera, Bacillus, Acidovorax, Oceanobacillus and Lactococcus) were associated with hrHPV-infected pregnant women. Conclusions: This work uncovered a probable synergistic effect of hrHPV infection and pregnancy on the vaginal microbial composition. HPV infection in pregnant women was associated with a more complex and diverse microbial environment.

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The In Vitro Effectiveness of Lactobacillus fermentum Against Different Candida Species Compared With Broadly Used Azoles

Abstract: The use of beneficial bacteria, especially lactobacilli, could be regarded as a good alternative for the prevention and treatment of Candida infections.

Cited by 11 publications

References 15 publications

A prospective longitudinal study on the microbiota composition in amyotrophic lateral sclerosis

A prospective longitudinal study on the microbiota composition in amyotrophic lateral sclerosis

Human papillomavirus infection and cervical intraepithelial neoplasia progression are associated with increased vaginal microbiome diversity in a Chinese cohort

Association between the vaginal microbiome and high-risk human papillomavirus infection in pregnant Chinese women

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