Antibacterial activity of six medicinal Cameroonian plants against Gram-positive and Gram-negative multidrug resistant phenotypes

Voukeng, Igor K.; Beng, Véronique Penlap; Kuete, Victor

doi:10.1186/s12906-016-1371-y

Cited by 66 publications

(37 citation statements)

References 56 publications

(44 reference statements)

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“…However, the MIC values reported in these studies are different from the values being reported here. This can be attributed to the fact that the strains used in these studies are not the same and different strains of the same species are known to have different sensitivities for an antimicrobial agent (Hiramatsu, ; Voukeng, Beng, & Kuete, ). However, to the best of our knowledge, there are no reports on the antibacterial activity of RL against the strains of E. faecium (ATCC 49224 and ATCC 29212) and MRSA (ATCC 562 and ATCC 43300).…”

Section: Resultsmentioning

confidence: 99%

Characterization of Biosurfactant Produced by a Novel Strain ofPseudomonas aeruginosa, Isolate ADMT1

Tiwary

Dubey

2018

J Surfact & Detergents

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Several biosurfactant-producing bacterial strains were isolated from petroleum-contaminated soil. The isolate ADMT1, identified as a new strain of Pseudomonas aeruginosa, was selected for further studies on the basis of oil displacement test and emulsification index (E24). The optimal parameters for production, determined by employing Box-Behnken design, were temperature 36.5 C and pH 7. The environmental isolate ADMT1 produced significant amount of biosurfactant (1.7 g L −1 in 72 h) in minimal salt medium (MSM) using dextrose as the sole carbon source. The E24 value and critical micelle concentration (CMC) of the biosurfactant was 100% and 150 mg L −1 , respectively. At CMC, the surface tension of water was reduced to 28.4 mN m −1 . The biosurfactant exhibited hemolytic activity and antibacterial activity against 8 reference strains of pathogenic bacteria, including 2 methicillin-resistant Staphylococcus aureus strains (MRSA ATCC 562 and MRSA ATCC 43300), with minimum inhibitory concentration (MIC) of 0.4 and 0.2 mg mL −1 , respectively. The structure of biosurfactant was characterized by FTIR, 1 H, and 13 C NMR spectroscopy. 7 di-rhamnolipid (RL) congeners were identified in the biosurfactant by ultraperformance liquid chromatographymass spectrometry analysis. The major congeners, which constituted 67% of the RL mixture, included Rha-Rha-C 10

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Section: Resultsmentioning

confidence: 99%

Characterization of Biosurfactant Produced by a Novel Strain ofPseudomonas aeruginosa, Isolate ADMT1

Tiwary

Dubey

2018

J Surfact & Detergents

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“…They were obtained clinically and from the American Type Culture Collection (ATCC). Their bacterial features were previously reported (Additional file 1: Table S1) [6]. Mueller Hinton Agar (Sigma) was used to activate the microorganisms whilst Mueller Hinton broth (MHB; Sigma) was used for antibacterial assays [16].…”

Section: Methodsmentioning

confidence: 99%

“…Recently, several bioactive plants against MDR bacteria were reported. Some of these include Erythrina sigmoidea [4], Cinnamomum zeylanicum, Aframomum citratum , Paullinia pinnata [5, 6], Allanblackia gabonensis , Combretum molle [7] and Harungana madagascariensis [8]. In our continous search of antibacterials from plants used traditionnally to manage microbial infections, we targeted Elaeophorbia drupifera (Thonn.)…”

Section: Introductionmentioning

confidence: 99%

Antibacterial activities of the methanol extract, fractions and compounds from Elaeophorbia drupifera (Thonn.) Stapf. (Euphorbiaceae)

Voukeng

Nganou

Sandjo

et al. 2017

BMC Complement Altern Med

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Background Elaeophorbia drupifera (Thonn.) Stapf. (Euphorbiaceae) is used in Cameroonian folk medicine to treat several ailments including bacterial-related diseases such as skin infections. In this study, the methanol extract from the leaves (EDL), fractions (EDLa-d), sub-fractions EDLc1-7 and EDLc31-35 as well as isolated compounds were tested for their antimicrobial activities against a panel of Gram-negative and Gram-positive bacteria including multidrug resistant (MDR) phenotypes.MethodsThe broth microdilution method was used to determine the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of the above samples; column chromatography was used for the fractionation and purification of the leaves extract whilst the chemical structures of compounds were determined using spectroscopic techniques.ResultsPhytochemical investigation lead to the isolation of a mixture (1:3) of stigmasterol and β-sitosterol (1 + 2), euphol (3), sitosterol-O-β- D -xylopyranoside (4), 3,3′,4′-tri-O-methylellagic acid (5), a mixture (1:1) of afzelin and quercetin-3-O-β-D-xylopyranoside (6 + 7), 3,3′,4′-tri-O-methylellagic acid 4-O-β-D-glucopyranoside (8), ellagic acid-4-O-β-xylopyranoside-3,3′,4′-trimethyl ether (9) from EDLc. Crude extract and fractions displayed selective activities with MIC values ranged from 32 to 1024 μg/mL for EDL against 84.9% of the 33 tested bacteria, 93.9% for EDLc, 69.7% for EDLb, 33.4% for EDLa and 0.03% for EDLd. MIC values ranged from 16 to 1024 μg/mL were obtained with EDLc3 and EDLc4 on all tested bacteria meanwhile other sub-fractions displayed selective activities. MIC value of 32 μg/mL was obtained with fractions EDLa against Escherichia coli AG100, EDLc against Enterobacer aerogenes ATCC13048 and EA298. For sub-fractions obtained from EDLc, the lowest MIC value of 16 μg/mL was recorded with EDLc3 against Staphylococcus aureus MRSA11. A corresponding value of 8 μg/mL against Providencia stuartii NAE16 was recorded with EDLc33 obtained from further fractionation of EDLc3. EDLc3 had MIC values below 100 μg/mL against all tested bacteria. Compound 5 as well as the mixture (1:1) of 6 and 7 inhibited the growth of all the tested bacteria with MICs ranged from 64 to 256 μg/mL.Conclusion Elaeophorbia drupifera is a potential source of phytomedicine to tackle MDR bacteria. Sub-fraction EDLc3 was more active than all isolated compounds and deserves further investigations to develop natural drug to combat Gram-negative, Gram-positive bacteria and otherwise MDR phenotypes.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-016-1509-y) contains supplementary material, which is available to authorized users.

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“…Their bacterial features were previous reported [6]. Mueller–Hinton Agar (Sigma) was used to activate the microorganisms whilst Mueller Hinton broth (MHB; Sigma) was used for antibacterial assays.…”

Section: Methodsmentioning

confidence: 99%

Multidrug resistant bacteria are sensitive to Euphorbia prostrata and six others Cameroonian medicinal plants extracts

2017

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BackgroundMultidrug resistant (MDR) bacteria are responsible for therapeutic failure and there is an urgent need for novels compounds efficient on them.MethodsEleven methanol extracts from seven Cameroonian medicinal plants were tested for their antibacterial activity using broth micro-dilution method against 36 MDR bacterial strains including Escherichia coli, Enterobacter aerogenes, Enterobacter cloacae, Klebsiella pneumoniae, Providencia stuartii, Pseudomonas aeruginosa and Staphylococcus aureus.Results Euphorbia prostrata extract was found active against all the 36 tested bacteria including Gram-negative phenotypes over-expressing efflux pumps such as P. aeruginosa PA124, E. aerogenes CM64 and E. coli AG102. E. prostrata had minimal inhibitory concentrations values between 128 and 256 µg/mL on 55.55% of the studied microorganisms. Other plants extract displayed selective antibacterial activity.ConclusionsResults obtained in this study highlight the antibacterial potential of the tested plants and the possible use of E. prostrata to combat bacterial infections including MDR phenotypes.

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Antibacterial activity of six medicinal Cameroonian plants against Gram-positive and Gram-negative multidrug resistant phenotypes

Cited by 66 publications

References 56 publications

Characterization of Biosurfactant Produced by a Novel Strain ofPseudomonas aeruginosa, Isolate ADMT1

Characterization of Biosurfactant Produced by a Novel Strain ofPseudomonas aeruginosa, Isolate ADMT1

Antibacterial activities of the methanol extract, fractions and compounds from Elaeophorbia drupifera (Thonn.) Stapf. (Euphorbiaceae)

Multidrug resistant bacteria are sensitive to Euphorbia prostrata and six others Cameroonian medicinal plants extracts

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