Effect of diets supplemented with different conjugated linoleic acid (CLA) isomers on protein expression in C57/BL6 mice

Casa, L. Della; Rossi, Elena; Romanelli, C.; Gibellini, Lara; Iannone, Anna

doi:10.1186/s12263-016-0542-2

Cited by 7 publications

(7 citation statements)

References 35 publications

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“…Several reports have demonstrated that it is the t10, c12 CLA isomer that is responsible for liver steatosis development, and that the mechanism appears to be due to increased circulating free fatty acids and glucose that are picked up by the liver, and not adequately oxidized [65]. Proteomic and molecular analyses of fatty liver in mice treated with CLA have shown increases in gene expression and levels of proteins related to lipogenesis [65,66]. In our study, 100% of WT mice demonstrated liver steatosis on CLA diet, but only ~50% of PWS and PWS-KO mice did.…”

Section: Discussionmentioning

confidence: 99%

Dietary Conjugated Linoleic Acid Reduces Body Weight and Fat in Snord116m+/p− and Snord116m−/p− Mouse Models of Prader–Willi Syndrome

et al. 2022

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Prader–Willi Syndrome (PWS) is a human genetic condition that affects up to 1 in 10,000 live births. Affected infants present with hypotonia and developmental delay. Hyperphagia and increasing body weight follow unless drastic calorie restriction is initiated. Recently, our laboratory showed that one of the genes in the deleted locus causative for PWS, Snord116, maintains increased expression of hypothalamic Nhlh2, a basic helix–loop–helix transcription factor. We have previously also shown that obese mice with a deletion of Nhlh2 respond to a conjugated linoleic acid (CLA) diet with weight and fat loss. In this study, we investigated whether mice with a paternal deletion of Snord116 (Snord116m+/p−) would respond similarly. We found that while Snord116m+/p− mice and mice with a deletion of both Snord116 alleles were not significantly obese on a high-fat diet, they did lose body weight and fat on a high-fat/CLA diet, suggesting that the genotype did not interfere with CLA actions. There were no changes in food intake or metabolic rate, and only moderate differences in exercise performance. RNA-seq and microbiome analyses identified hypothalamic mRNAs, and differentially populated gut bacteria, that support future mechanistic analyses. CLA may be useful as a food additive to reduce obesity in humans with PWS.

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Section: Discussionmentioning

confidence: 99%

Dietary Conjugated Linoleic Acid Reduces Body Weight and Fat in Snord116m+/p− and Snord116m−/p− Mouse Models of Prader–Willi Syndrome

et al. 2022

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“…An important issue that needs to be addressed is the mechanism through which CLA and exercise modulate liver profiles of FAs. A proteomic study showed that some specific CLA isomers might influence the activities of enzymes involved in beta-oxidation and fructose metabolism (Della Casa et al, 2016). Furthermore, exercising muscles release various myokines, and many of them have already been shown to influence the liver metabolism (Carson, 2017).…”

Section: Discussionmentioning

confidence: 99%

Exercise and Conjugated Linoleic Acid Supplementation Induce Changes in the Composition of Liver Fatty Acids

et al. 2019

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Exercise and supplementation with conjugated linoleic acid (CLA) are used to reduce body weight and to improve health. Applied together, they may exert a synergistic effect. However, the effects of exercise and CLA supplementation on liver metabolism are poorly understood. The aim of this study was to examine the influence of exercise and CLA supplementation on fatty acids (FA) composition in mouse liver. We analyzed 44 of known FAs of this organ by gas chromatography-mass spectrometry. Our results demonstrated that exercise contributed to a decrease in odd-chain FA and an increase in n-6 polyunsaturated FA contents. In turn, CLA stimulated an increase in branched-chain FAs and n-3 polyunsaturated FAs. Exercise combined with CLA supplementation caused a substantial increase in branched-chain FA content and a slight increase in n-6 polyunsaturated FAs. The elevated liver content of branched-chain FAs after the exercise combined with CLA supplementation, as well as the increase in n-3 polyunsaturated FAs after CLA may be favorable since both these FA groups were previously shown to produce health benefits. However, the expression pattern of enzymes involved in fatty acid synthesis did not match the changes in FA composition. Thus, the mechanism of exercise- and CLA-induced changes in liver FA profile is yet to be established. Also, the consequences of CLA- and/or exercise-induced changes in the composition of liver FAs need to be elucidated.

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“…When taken as a dietary supplement, CLAs exhibit numerous beneficial health effects that are sometimes dependent on the isomer, including anti-carcinogenic, anti-inflammatory and anti-obesity effects [ 12 , 17 ]. Nevertheless, these beneficial effects are, in some instances, associated with adverse effects, such as adipose tissue inflammation [ 19 , 20 ] or hepatic steatosis, which occurs following stimulation of fatty acid synthesis [ 21 – 23 ]. However, controversies exist since some isomers also induce concomitant activation of the fatty acid oxidative pathway [ 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Implication of trans-11,trans-13 conjugated linoleic acid in the development of hepatic steatosis

et al. 2018

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ScopeConjugated linoleic acids are linoleic acid isomers found in the diet that can also be produced through bacterial metabolism of polyunsaturated fatty acids. Our objective was to evaluate the contribution of fatty acid metabolites produced from polyunsaturated fatty acids by the gut microbiota in vivo to regulation of hepatic lipid metabolism and steatosis.Methods and resultsIn mice with depleted n-3 polyunsaturated fatty acids, we observed an accumulation of trans-11,trans-13 CLA and cis-9,cis-11 conjugated linoleic acids in the liver tissue that were associated with an increased triglyceride content and expression of lipogenic genes. We used an in vitro model to evaluate the impact of these two conjugated linoleic acids on hepatic lipid metabolism. In HepG2 cells, we observed that only trans-11,trans-13 conjugated linoleic acids recapitulated triglyceride accumulation and increased lipogenic gene expression, which is a phenomenon that may implicate the nuclear factors sterol regulatory element binding protein 1c (SREBP-1c) and carbohydrate-responsive element-binding protein (ChREBP).ConclusionThe trans-11,trans-13 conjugated linoleic acids can stimulate hepatic lipogenesis, which supports the conclusion that gut microbiota and related metabolites should be considered in the treatment of non-alcoholic liver disease.

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Effect of diets supplemented with different conjugated linoleic acid (CLA) isomers on protein expression in C57/BL6 mice

Cited by 7 publications

References 35 publications

Dietary Conjugated Linoleic Acid Reduces Body Weight and Fat in Snord116m+/p− and Snord116m−/p− Mouse Models of Prader–Willi Syndrome

Dietary Conjugated Linoleic Acid Reduces Body Weight and Fat in Snord116m+/p− and Snord116m−/p− Mouse Models of Prader–Willi Syndrome

Exercise and Conjugated Linoleic Acid Supplementation Induce Changes in the Composition of Liver Fatty Acids

Implication of trans-11,trans-13 conjugated linoleic acid in the development of hepatic steatosis

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