Direct detection ofmecA,bla_SHV, bla_CTX-M,bla_TEMandbla_OXAgenes from positive blood culture bottles by multiplex-touchdown PCR assay

Abstract

Many studies on the development of PCR for the detection of resistance genes have already been published, including multiplex PCR methods. However, cross-amplification reactions can be a major concern in multiplex PCR methods. In this study, we developed a highly sensitive and specific multiplex-touchdown PCR assay for simultaneous detection of mecA, bla , bla , bla and bla genes from positive blood culture bottles, cross-amplification was absent and false-positive results were not obtained.