Real‐Time Label‐Free Monitoring of Nanoparticle Cell Uptake

Suutari, Teemu; Silen, Tiina; Karaman, Didem Şen; Saari, H; Desai, Dawood; Kerkelä, Erja; Laitinen, Saara; Hanzlíková, Martina; Rosenholm, Jessica M.; Yliperttula, Marjo; Viitala, Tapani

doi:10.1002/smll.201601815

Cited by 28 publications

(40 citation statements)

References 46 publications

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“…6B) was not a result of intracellular saturation of accumulated PEG0.1-NPs. This plateau could therefore be indicative of a reduced activity of endocytic mechanisms for intracellular uptake that results from PEG0.1-NP exposure, as observed with other types of NPs [62]. With these results, we think that the cytotoxicity observed at higher concentrations of PEG0.1-NPs is probably due to the increased accumulation of NPs within intracellular compartments, such as lysosomes, resulting in oxidative stress and triggering cell apoptosis [63].…”

Section: Cellular Uptake Of Peg01-npsmentioning

confidence: 71%

Nanocarrier systems assembled from PEGylated hyperbranched poly(arylene oxindole)

Al‐Halifa

Lambrechts

Verheyen

et al. 2019

European Polymer Journal

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Nanoparticles (NPs) formed from hyperbranched polymers are highly attractive organized structures in nanomedicine. Herein, we utilize copper-catalyzed azide-alkyne cycloaddition of hyperbranched poly(arylene oxindole) derivatives to prepare amphiphilic conjugates with different degrees of PEGylation that form spherical NPs in water with tunable particle diameters (25 ± 5 nm for PEG0.3-NPs and 120 ± 10 nm for PEG0.1-NPs) and zeta potential (-7 ± 2 mV for PEG0.3-NPs and-23 ± 3 mV for PEG0.1-NPs). Cisplatin and BODIPY derivatives can be loaded into the PEG0.1-NPs, and their release over 5 d depends on the physicochemical properties of the payload. Moreover, changing the time and conditions for self-assembly leads to the formation of polymer vesicles that can encapsulate and release hydrophilic cargo, as shown with fluorescein. In vitro cellular uptake experiments demonstrate that at concentrations ≤10 g/mL the BODIPY-loaded NPs do not induce morphological differences or cause a reduction in metabolic activity of the cells, confirming their cytocompatibility. They are rapidly taken up by ATDC5 cells and remain stable for 7 d thus highlighting their potential as carrier systems for diagnostic or therapeutic applications.

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Section: Cellular Uptake Of Peg01-npsmentioning

confidence: 71%

Nanocarrier systems assembled from PEGylated hyperbranched poly(arylene oxindole)

Al‐Halifa

Lambrechts

Verheyen

et al. 2019

European Polymer Journal

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“…The plasma membrane represents the final barrier through which therapeutic agents must penetrate to enter the cell, and the permeability of hydrophilic, small-molecular drugs is usually poor. Because uptake mechanisms like simple or facilitated diffusion, active transport, or even endocytosis are often insufficient, external delivery systems such as cell-penetrating peptides (CPPs), antibodies, or liposomes might be used to improve delivery and cell uptake of peptides [38][39][40]. However, at least for peptides δ-111, δ-281, and δ-371 used in our experimental approach, extensive cell entry into differentiated hNPCs could be observed without applying any further modifications on peptide sequence or delivery.…”

Section: Discussionmentioning

confidence: 99%

CK1δ-Derived Peptides as Novel Tools Inhibiting the Interactions between CK1δ and APP695 to Modulate the Pathogenic Metabolism of APP

Roth

Gärtner

Mayer

et al. 2021

IJMS

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Alzheimer’s disease (AD) is the major cause of dementia, and affected individuals suffer from severe cognitive, mental, and functional impairment. Histologically, AD brains are basically characterized by the presence of amyloid plaques and neurofibrillary tangles. Previous reports demonstrated that protein kinase CK1δ influences the metabolism of amyloid precursor protein (APP) by inducing the generation of amyloid-β (Aβ), finally contributing to the formation of amyloid plaques and neuronal cell death. We therefore considered CK1δ as a promising therapeutic target and suggested an innovative strategy for the treatment of AD based on peptide therapeutics specifically modulating the interaction between CK1δ and APP. Initially, CK1δ-derived peptides manipulating the interactions between CK1δ and APP695 were identified by interaction and phosphorylation analysis in vitro. Selected peptides subsequently proved their potential to penetrate cells without inducing cytotoxic effects. Finally, for at least two of the tested CK1δ-derived peptides, a reduction in Aβ levels and amyloid plaque formation could be successfully demonstrated in a complex cell culture model for AD. Consequently, the presented results provide new insights into the interactions of CK1δ and APP695 while also serving as a promising starting point for further development of novel and highly innovative pharmacological tools for the treatment of AD.

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“…Also, the cell viability was 52% at 40 μg/mL high concentration of rGO-PEG-FA nanomaterial. This result indicates that the cellular uptake of the nanoparticles is saturated [72,73]. Therefore, we optimized at 30 μg/ mL concentration of rGO-PEG-FA nanomaterials to apply photothermal therapy.…”

Section: Photothermal Therapy Effects In Microfluidic Co-culture Platmentioning

confidence: 99%

rGO nanomaterial-mediated cancer targeting and photothermal therapy in a microfluidic co-culture platform

et al. 2020

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We developed the microfluidic co-culture platform to study photothermal therapy applications. We conjugated folic acid (FA) to target breast cancer cells using reduced graphene oxide (rGO)-based functional nanomaterials. To characterize the structure of rGO-based nanomaterials, we analyzed the molecular spectrum using UV-visible and Fouriertransform infrared spectroscopy (FT-IR). We demonstrated the effect of rGO-FA-based nanomaterials on photothermal therapy of breast cancer cells in the microfluidic co-culture platform. From the microfluidic co-culture platform with breast cancer cells and human umbilical vein endothelial cells (HUVECs), we observed that the viability of breast cancer cells treated with rGO-FA-based functional nanomaterials was significantly decreased after near-infrared (NIR) laser irradiation. Therefore, this microfluidic co-culture platform could be a potentially powerful tool for studying cancer cell targeting and photothermal therapy.

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Real‐Time Label‐Free Monitoring of Nanoparticle Cell Uptake

Cited by 28 publications

References 46 publications

Nanocarrier systems assembled from PEGylated hyperbranched poly(arylene oxindole)

Nanocarrier systems assembled from PEGylated hyperbranched poly(arylene oxindole)

CK1δ-Derived Peptides as Novel Tools Inhibiting the Interactions between CK1δ and APP695 to Modulate the Pathogenic Metabolism of APP

rGO nanomaterial-mediated cancer targeting and photothermal therapy in a microfluidic co-culture platform

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