<i>TWIST1</i>/miR-584/<i>TUSC2</i> pathway induces resistance to apoptosis in thyroid cancer cells

Orlandella, Francesca Maria; Maro, Gennaro Di; Ugolini, Clara; Basolo, Fulvio; Salvatore, Giuliana

doi:10.18632/oncotarget.12129

Cited by 27 publications

(29 citation statements)

References 50 publications

(56 reference statements)

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“…Additionally, miR-584 is an independent prognostic factor for the outcome in neuroblastoma patients (38). Downregulation of miR-584 was also observed in clear cell renal cell carcinoma (21), thyroid carcinoma (22,23) and glioma (24). These findings suggested that aberrant expression of miR-584 is a potential prognostic factor in these cancer types.…”

Section: Discussionmentioning

confidence: 99%

“…Ueno et al revealed that miR-584 overexpression decreased cell motility in clear cell renal cell carcinoma (21). Moreover, Orlandella et al showed that resumed expression of miR-584 inhibited the migration and invasion of thyroid carcinoma cells and induced their resistance to apoptosis (22,23). Wang et al indicated that miR-584 reexpression repressed cell groWTh, migration, invasion and vasculogenic mimicry of glioma (24,39,40).…”

Section: Discussionmentioning

confidence: 99%

“…Multiple targets of miR-584 have been validated, including WWP1 (37) in gastric cancer, MMP14 (38) in neuroblastoma, ROCK1 (21) in clear cell renal cell carcinoma, TUSC2 (23) and ROCK1 (22) in thyroid carcinoma, and PTTG1IP (24) and ROCK1 (39,40) in glioma. These studies suggest that miR-584 exhibits tissue-specific functions.…”

Section: Discussionmentioning

confidence: 99%

“…MiR-584 functions as tumour suppressor in several types of cancer (21)(22)(23)(24). In NSCLC, miR-584 expression was reported to be downregulated in tissue samples (25) and upregulated in the plasma of NSCLC patients (26).…”

Section: Microrna-584 Inhibits Cell Proliferation and Invasion In Nonmentioning

confidence: 99%

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MicroRNA-584 inhibits cell proliferation and invasion in non-small cell lung cancer by directly targeting MTDH

Zhang

Wang

2017

Exp Ther Med

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Abstract. Lung cancer is the third most frequent human malignant tumour and the leading cause of cancer-associated mortality worldwide. Emerging lines of evidence have demonstrated that microRNAs (miRNAs) are upregulated or downregulated in non-small cell lung cancer (NSCLC), and this phenomenon is involved in the regulation of various processes during tumorigenesis and progression, including tumour groWTh, apoptosis, cell invasion, and tumour metastasis. Therefore, understanding the molecular mechanism that associates abnormally expressed miRNAs with NSCLC formation and development may lead to the identification of novel diagnostic, and therapeutic targets for patients with NSCLC. miRNA-584 (miR-584) functions as a tumour suppressor in several types of cancer. However, the expression pattern, detailed biological function and underlying molecular mechanism of miR-584 in NSCLC remain unclear. Therefore, the present study detected the expression of miR-584 in NSCLC, investigated its role in NSCLC cells and determined its underlying molecular mechanism. In the current study, it was demonstrated that miR-584 was downregulated in NSCLC tissues and cell lines. Low miR-584 expression was correlated with tumour size, tumour node metastasis stage and distant metastasis. Overexpression of miR-584 inhibited cell proliferation and invasion in NSCLC. Additionally, metadherin was identified as a direct target gene of miR-584 in NSCLC as confirmed by a series of experiments. Moreover, upregulation of miR-584 was involved in the regulation of the phosphatase and tensin homolog/Akt serine/threonine kinase signalling pathway in NSCLC. Thus, miR-584 may serve as a tumor-suppressor, and the results of the present study provide a reference for future research into the potential mechanisms underlying NSCLC progression.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Microrna-584 Inhibits Cell Proliferation and Invasion In Nonmentioning

confidence: 99%

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MicroRNA-584 inhibits cell proliferation and invasion in non-small cell lung cancer by directly targeting MTDH

Zhang

Wang

2017

Exp Ther Med

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“…1A. TPC-1 is an established human PTC cell line [25, 26]. TPC-1 cells were cultured in FBS-containing complete medium, and were treated with different concentrations (10-1000 nM) of CZ415.…”

Section: Resultsmentioning

confidence: 99%

The mTOR Kinase Inhibitor CZ415 Inhibits Human Papillary Thyroid Carcinoma Cell Growth

Song

et al. 2018

Cell Physiol Biochem

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Background/Aim: Mammalian target of rapamycin (mTOR) plays an important role in papillary thyroid carcinoma (PTC) cell progression. CZ415 is a novel, highly-efficient and specific mTOR kinase inhibitor. The current study tested the potential anti-tumor activity of CZ415 in human PTC cells. Methods: The established (TPC-1 cell line) and primary human PTC cells were treated with CZ415. Cell survival and growth were tested by Cell Counting Kit-8 assay and BrdU ELISA assay, respectively. Cell apoptosis was tested by caspase-3/-9 activity assay, Hoechst-33342 staining assay and single-stranded DNA ELISA assay. Cell cycle progression was tested by propidium iodide-FACS assay. The mTOR signaling was tested by Western blotting assay and co-immunoprecipitation assay. The mouse xenograft tumor model was applied to study the effect of CZ415 in vivo. Results: In cultured human PTC cells, treatment with CZ415 at nM concentrations significantly inhibited cell survival and growth. CZ415 induced apoptosis activation and cell cycle arrest in human PTC cells. CZ415 disrupted assembling of mTORC1 (mTOR-Raptor association) and mTORC2 (mTOR-Rictor-GβL association) in TPC-1 cells, which led to de-phosphorylation of the mTORC1 substrates (S6K1 and 4E-BP1) and the mTORC2 substrate AKT (Ser-473). Further studies show that the autophagy inhibitor 3-methyladenine (3-MA) or Beclin-1 shRNA aggravated CZ415-induced cytotoxicity against PTC cells. In vivo, CZ415 oral administration inhibited TPC-1 xenograft tumor growth in mice. Conclusion: Our results show that mTOR blockage by CZ415 inhibits PTC cell growth in vitro and in vivo.

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Immunohistochemical and molecular evaluation of TUSC2 expression in breast cancer

Tekin,

Edgünlü,

Genç

2024

Mol Biol Rep

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TWIST1/miR-584/TUSC2 pathway induces resistance to apoptosis in thyroid cancer cells

Cited by 27 publications

References 50 publications

MicroRNA-584 inhibits cell proliferation and invasion in non-small cell lung cancer by directly targeting MTDH

MicroRNA-584 inhibits cell proliferation and invasion in non-small cell lung cancer by directly targeting MTDH

The mTOR Kinase Inhibitor CZ415 Inhibits Human Papillary Thyroid Carcinoma Cell Growth

Immunohistochemical and molecular evaluation of TUSC2 expression in breast cancer

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