Draft Genome Sequences of 40 Salmonella enterica Serovar Typhimurium Strains Isolated from Humans and Food in Brazil

Almeida, Fernanda Martins de; Medeiros, Marta Inês Cazentini; Rodrigues, Dália P.; Payne, Justin L.; Timme, Ruth; Allard, Marc W.; Falcão, Juliana Pfrimer

doi:10.1128/genomea.00892-16

Cited by 5 publications

(6 citation statements)

References 9 publications

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“…The N 50 values of the two assemblies were 278,931 bp and 357,417 bp for VA-WGS-00333 and VA-WGS-00353, respectively. The GC content values (VA-WGS-00333, 52.07%; VA-WGS-00353, 52.18%) and genome lengths (VA-WGS-00333, 4.6 Mb; VA-WGS-00353, 4.7 Mb) were consistent with other reported Salmonella genomes ( 9 , 10 ).…”

Section: Genome Announcementsupporting

confidence: 87%

Draft Genome Sequences of Two Salmonella Strains Isolated from Wild Animals on the Eastern Shore of Virginia

Libuit

Turner

2018

Genome Announc

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Section: Genome Announcementsupporting

confidence: 87%

Draft Genome Sequences of Two Salmonella Strains Isolated from Wild Animals on the Eastern Shore of Virginia

Libuit

Turner

2018

Genome Announc

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“…The concentration of the genomic DNA was detected in NanoDrop 1000 (Thermo Scientific). Libraries were prepared using 1 ng of genomic DNA with the Nextera XT DNA library preparation kit (Illumina, San Diego, CA) [30].…”

Section: Bacterial Strains and Genome Sequencingmentioning

confidence: 99%

“…The WGS was performed using the NextSeq 500 desktop sequencer with the NextSeq 500/500 high-output version 2 kit (Illumina) for 2 × 151 cycles according to the manufacturer's instructions in the US Food and Drug Administration (FDA), College Park, MD, USA [30].…”

Section: Bacterial Strains and Genome Sequencingmentioning

confidence: 99%

Phylogenetic analysis revealed that Salmonella Typhimurium ST313 isolated from humans and food in Brazil presented a high genomic similarity

et al. 2019

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Salmonella Typhimurium sequence type 313 (S. Typhimurium ST313) has caused invasive disease mainly in sub-Saharan Africa. In Brazil, ST313 strains have been recently described, and there is a lack of studies that assessed by whole genome sequencing (WGS)-the relationship of these strains. The aims of this work were to study the phylogenetic relationship of 70 S. Typhimurium genomes comparing strains of ST313 (n = 9) isolated from humans and food in Brazil among themselves, with other STs isolated in this country (n = 31) and in other parts of the globe (n = 30) by 16S rRNA sequences, the Gegenees software, whole genome multilocus sequence typing (wgMLST), and average nucleotide identity (ANI) for the genomes of ST313. Additionally, pangenome analysis was performed to verify the heterogeneity of these genomes. The phylogenetic analyses showed that the ST313 genomes were very similar among themselves. However, the ST313 genomes were usually clustered more distantly to other STs of strains isolated in Brazil and in other parts of the world. By pangenome calculation, the core genome was 2,880 CDSs and 4,171 CDSs singletons for all the 70 S. Typhimurium genomes studied. Considering the 10 ST313 genomes analyzed the core genome was 4,112 CDSs and 76 CDSs singletons. In conclusion, the ST313 genomes from Brazil showed a high similarity among them which information might eventually help in the development of vaccines and antibiotics. The pangenome analysis showed that the S. Typhimurium genomes studied presented an open pangenome, but specifically tending to become close for the ST313 strains.

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“…Typhimurium strains in Brazil have become available very recently (Almeida et al . ; Panzenhagen et al . ), and limited information about their genome insight was revealed so far.…”

Section: Introductionmentioning

confidence: 99%

Comparative genome analysis and characterization of theSalmonellaTyphimurium strain CCRJ_26 isolated from swine carcasses using whole-genome sequencing approach

Panzenhagen

Cabral

Suffys

et al. 2018

Lett Appl Microbiol

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Draft Genome Sequences of 40 Salmonella enterica Serovar Typhimurium Strains Isolated from Humans and Food in Brazil

Cited by 5 publications

References 9 publications

Draft Genome Sequences of Two Salmonella Strains Isolated from Wild Animals on the Eastern Shore of Virginia

Draft Genome Sequences of Two Salmonella Strains Isolated from Wild Animals on the Eastern Shore of Virginia

Phylogenetic analysis revealed that Salmonella Typhimurium ST313 isolated from humans and food in Brazil presented a high genomic similarity

Comparative genome analysis and characterization of theSalmonellaTyphimurium strain CCRJ_26 isolated from swine carcasses using whole-genome sequencing approach

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