Data on clinical significance of second trimester inflammatory biomarkers in the amniotic fluid in predicting preterm delivery

Abstract

In this article second trimester amniotic fluid biomarkers are measured for correlation with preterm delivery. One additional milliliter of amniotic fluid is collected during amniocentesis for dosages of IL-6, MMP-9, CRP and glucose levels, along with maternal serum CRP and glucose. MMP-9 and Il-6 levels were measured with the corresponding Human QuantikineR ELISA Kit (R&D systems) according to the instructions provided by the manufacturer. Cut-off values for AF MMP-9 and IL-6 were fixed by the kit sensitivity thresholds.Data includes ROC curves for glucose (Fig. 1), IL-6 (Fig. 2) and MMP-9 (Fig. 3), aiming to search for sensitivity and specificity in the prediction of premature delivery. Statistical analyses are performed with SPSS v20.0 software. Statistical significance is determined using the Mann–Whitney and one way ANOVA test. The association with preterm delivery is performed using a two proportions test. Correlations are measured using the Pearson׳’s coefficient. A p value<0.05 is considered statistically significant. The data is presented in the figures provided. Data relied on a previous publication “Prediction of preterm delivery by second trimester inflammatory biomarkers in the amniotic fluid” (A. Kesrouani, E. Chalhoub, E. El Rassy, M. Germanos, A. Khazzaka, J. Rizkallah, E. Attieh, N. Aouad, 2016) [1].

The association between selected mid-trimester amniotic fluid candidate proteins and spontaneous preterm delivery

“…The results of previous studies are conflicting, most likely due to dissimilarities in study design and diversities in study populations [26]. Some of the previous studies have reported higher levels of inflammatory biomarkers in mid-trimester amniotic fluid in women with a subsequent spontaneous PTD [21,23,37,[40][41][42][43][47][48][49][50]53,54,58], whereas other studies did not find any significant differences [25,26,38,39,[44][45][46]51,52,55,59]. Our study design, definition of groups and gestational age at sampling were most similar to the research by Bamberg et al [38] and Puchner et al [51], who found lower, but not significantly lower, levels of selected inflammatory mediators in women with spontaneous PTD, compared with women delivering at term.…”

Investigating the predictive role of uterocervical angle in predicting preterm labor in singleton pregnancies: a meta-analysis

“…In recent years, different measurements and tests have been developed for the prediction of preterm labor [2]. The leading ones include cervical length measurement, fetal fibronectin testing, and biochemical markers obtained from maternal serum and amnion [4]. Maternal blood and amniotic fluid inflammatory markers may cause anxiety in pregnant women due to the interventional nature of the procedures.…”