Nuclear Localised MORE SULPHUR ACCUMULATION1 Epigenetically Regulates Sulphur Homeostasis in Arabidopsis thaliana

Huang, Xin‐Yuan; Chao, Dai‐Yin; Kopřivová, Anna; Danku, John; Wirtz, Markus; Müller, Susanne; Sandoval, Francisco J.; Bauwe, Hermann; Roje, Sanja; Dilkes, Brian P.; Hell, Rüdiger; Kopriva, Stanislav; Salt, David E.

doi:10.1371/journal.pgen.1006298

Cited by 77 publications

(68 citation statements)

References 78 publications

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“…Moreover, OsCADT1 and MSA1 proteins are both localized to the nucleus (Fig. a; Huang et al , ). Nucleus localization is critical for the role of MSA1 in S assimilation (Huang et al , ).…”

Section: Discussionmentioning

confidence: 99%

Mutation in OsCADT1 enhances cadmium tolerance and enriches selenium in rice grain

et al. 2020

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Summary How cadmium (Cd) tolerance in rice is regulated remains poorly understood. We used a forward genetic approach to investigate Cd tolerance in rice. Using a root elongation assay, we isolated a rice mutant with enhanced Cd tolerance, cadt1, from an ethyl methanesulphonate (EMS)‐mutagenized population of a widely grown Indica cultivar. The mutant accumulated more Cd in roots but not in shoots and grains. Using genomic resequencing and complementation, we identified OsCADT1 as the causal gene for the mutant phenotype, which encodes a putative serine hydroxymethyltransferase. OsCADT1 protein was localized to the nucleus and the OsCADT1 gene was expressed in both roots and shoots. OsCADT1 mutation resulted in higher sulphur and selenium accumulation in the shoots and grains. Selenate influx in cadt1 was 2.4 times that of the wild‐type. The mutant showed higher expression of the sulphate/selenate transporter gene OsSULTR1;1 and the sulphur‐deficiency‐inducible gene OsSDI1. Thiol compounds including cysteine, glutathione and phytochelatins were significantly increased in the mutant, underlying its increased Cd tolerance. Growth and grain biomass were little affected. The results suggest that OsCADT1 acts as a negative regulator of sulphate/selenate uptake and assimilation. OsCADT1 mutation increases Cd tolerance and enriches selenium in rice grains, providing a novel solution for selenium biofortification.

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“…Moreover, OsCADT1 and MSA1 proteins are both localized to the nucleus (Fig. a; Huang et al , ). Nucleus localization is critical for the role of MSA1 in S assimilation (Huang et al , ).…”

Section: Discussionmentioning

confidence: 99%

Mutation in OsCADT1 enhances cadmium tolerance and enriches selenium in rice grain

et al. 2020

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“…This suggests that others root transporters, responding to S deficiency, may be involved or that activity of root sulfate transporters is also strongly regulated at the post-transcriptional level. Data on a double KO mutant for atsultr1;1 and atsultr1;2 can be found on the Purdue ionomics information management system (See [42] and http://www.ionomicshub.org/arabidopsis/piims/showIndex.action) as well as [43]. In this mutant, S and Mo contents are reduced compared to the wild type, suggesting that these two transporters could be involved in the uptake of S and Mo.…”

Section: Discussionmentioning

confidence: 99%

Non-Specific Root Transport of Nutrient Gives Access to an Early Nutritional Indicator: The Case of Sulfate and Molybdate

et al. 2016

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Under sulfur (S) deficiency, crosstalk between nutrients induced accumulation of other nutrients, particularly molybdenum (Mo). This disturbed balanced between S and Mo could provide a way to detect S deficiency and therefore avoid losses in yield and seed quality in cultivated species. Under hydroponic conditions, S deprivation was applied to Brassica napus to determine the precise kinetics of S and Mo uptake and whether sulfate transporters were involved in Mo uptake. Leaf contents of S and Mo were also quantified in a field-grown S deficient oilseed rape crop with different S and N fertilization applications to evaluate the [Mo]:[S] ratio, as an indicator of S nutrition. To test genericity of this indicator, the [Mo]:[S] ratio was also assessed with other cultivated species under different controlled conditions. During S deprivation, Mo uptake was strongly increased in B. napus. This accumulation was not a result of the induction of the molybdate transporters, Mot1 and Asy, but could be a direct consequence of Sultr1.1 and Sultr1.2 inductions. However, analysis of single mutants of these transporters in Arabidopsis thaliana suggested that other sulfate deficiency responsive transporters may be involved. Under field conditions, Mo content was also increased in leaves by a reduction in S fertilization. The [Mo]:[S] ratio significantly discriminated between the plots with different rates of S fertilization. Threshold values were estimated for the hierarchical clustering of commercial crops according to S status. The use of the [Mo]:[S] ratio was also reliable to detect S deficiency for other cultivated species under controlled conditions. The analysis of the leaf [Mo]:[S] ratio seems to be a practical indicator to detect early S deficiency under field conditions and thus improve S fertilization management.

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“…The growth condition was described previously (Huang et al, 2016b). A. thaliana transgenic plants were grown on MGRL agar media in a growth chamber at the University of Aberdeen, UK, as described (Huang et al, 2016a).…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Natural variation in a molybdate transporter controls grain molybdenum concentration in rice

et al. 2018

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Summary Molybdenum (Mo) is an essential micronutrient for most living organisms, including humans. Cereals such as rice (Oryza sativa) are the major dietary source of Mo. However, little is known about the genetic basis of the variation in Mo content in rice grain. We mapped a quantitative trait locus (QTL) qGMo8 that controls Mo accumulation in rice grain by using a recombinant inbred line population and a backcross introgression line population. We identified a molybdate transporter, OsMOT1;1, as the causal gene for this QTL. OsMOT1;1 exhibits transport activity for molybdate, but not sulfate, when heterogeneously expressed in yeast cells. OsMOT1;1 is mainly expressed in roots and is involved in the uptake and translocation of molybdate under molybdate‐limited condition. Knockdown of OsMOT1;1 results in less Mo being translocated to shoots, lower Mo concentration in grains and higher sensitivity to Mo deficiency. We reveal that the natural variation of Mo concentration in rice grains is attributed to the variable expression of OsMOT1;1 due to sequence variation in its promoter. Identification of natural allelic variation in OsMOT1;1 may facilitate the development of rice varieties with Mo‐enriched grain for dietary needs and improve Mo nutrition of rice on Mo‐deficient soils.

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Nuclear Localised MORE SULPHUR ACCUMULATION1 Epigenetically Regulates Sulphur Homeostasis in Arabidopsis thaliana

Cited by 77 publications

References 78 publications

Mutation in OsCADT1 enhances cadmium tolerance and enriches selenium in rice grain

Mutation in OsCADT1 enhances cadmium tolerance and enriches selenium in rice grain

Non-Specific Root Transport of Nutrient Gives Access to an Early Nutritional Indicator: The Case of Sulfate and Molybdate

Natural variation in a molybdate transporter controls grain molybdenum concentration in rice

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