Accelerated cell-surface interlocking on plasma polymer-modified porous ceramics

Rebl, Henrike; Finke, Birgit; Schmidt, Jürgen; Mohamad, Heba S.; Ihrke, Roland; Helm, Christiane A.; Nebe, J. Barbara

doi:10.1016/j.msec.2016.08.016

Cited by 25 publications

(31 citation statements)

References 34 publications

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“…Owing to these surface characteristics, enhanced cell spreading after PPAAm-coating was to be seen. We observed previously that the PPAAm coating promotes the initial cell spreading after seeding of the cells and increased the cell mobility ( Finke et al, 2007 ; Rebl et al, 2016 ). The PPAAm coating has proven to be a cell adhesive layer and osteoblasts can overcome topographical restrictions (e.g.…”

Section: Discussionmentioning

confidence: 91%

“…The PPAAm coating has proven to be a cell adhesive layer and osteoblasts can overcome topographical restrictions (e.g. contact guidance due to machined grooves) ( Rebl et al, 2012 , 2016 ). We observed that the osteoblastic cells try to internalize surface-fixed micropillars ( Moerke et al, 2016 ) and hypothesized that cells possibly increase their cell-surface contact, because this cell surface contact is essential for the maintenance of the osteoblast-specific cell function ( Saldaña and Vilaboa, 2010 ).…”

Section: Discussionmentioning

confidence: 99%

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Restricted cell functions on micropillars are alleviated by surface-nanocoating with amino groups

Moerke

Staehlke

Rebl

et al. 2018

Journal of Cell Science

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The topographical and chemical surface features of biomaterials are sensed by the cells, affecting their physiology at the interface. When placed on titanium, we recently discovered osteoblasts attempted caveolae-mediated phagocytosis of the sharp-edged microstructures. This active, energy-consuming process resulted in decreased osteoblastic cell functions (e.g. secretion of extracellular matrix proteins). However, chemical modification with plasma polymerized allylamine (PPAAm) was able to amplify osteoblast adhesion and spreading, resulting in better implant osseointegration in vivo. In the present in vitro study, we analyzed whether this plasma polymer nanocoating is able to attenuate the microtopography-induced changes of osteoblast physiology. On PPAAm, we found cells showed a higher cell interaction with the geometrical micropillars by 30 min, and a less distinct reduction in the mRNA expression of collagen type I, osteocalcin and fibronectin after 24 h of cell growth. Interestingly, the cells were more active and sensitive on PPAAm-coated micropillars, and react with a substantial Ca2+ ion mobilization after stimulation with ATP. These results highlight that it is important for osteoblasts to establish cell surface contact for them to perform their functions.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Restricted cell functions on micropillars are alleviated by surface-nanocoating with amino groups

Moerke

Staehlke

Rebl

et al. 2018

Journal of Cell Science

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“…Nevertheless, the success rate of titanium implants can still be improved. Inferior osteointegration caused by the bio-inert titanium surface threatens the long-term effi cacy of titanium implants 4) . Therefore, novel titanium implant materials may improve osteointegration by bioactive surfaces.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Osteointegration of a Novel Alkali-Treated Implant System <i>In Vivo</i>

Kusumoto

Yin

Zhang

et al. 2017

J. Hard Tissue Biology.

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“…[12][13][14][15][16] Furthermore, HAp nanomaterials have also been intensively investigated for many other biomedical applications, such as drug/gene delivery, bone tissue engineering. [17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34] Over the past few decades, HAp nanomaterials have been synthesized with different nanocrystal morphologies through hydrothermal treatment, like rod-like, spherical or needle-shaped crystals. [35][36][37] Though these HAp nanomaterials possess well controlled size and morphology, they are difficult to maintain good dispersion in water.…”

Section: Introductionmentioning

confidence: 99%

Surface grafting of Eu3+ doped luminescent hydroxyapatite nanomaterials through metal free light initiated atom transfer radical polymerization for theranostic applications

Zeng

Liu

Jiang

et al. 2017

Materials Science and Engineering: C

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We reported a simple and efficient method to prepare the hydrophilic luminescent HAp polymer nanocomposites through the combination of ligand exchange and metal free light initiated surface-initiated atom transfer radical polymerization (SI-ATRP) using 10-phenylphenothiazine (PTH) as organic catalyst and 2-methacryloyloxyethyl phosphorylcholine (MPC) and itaconic acid (IA) as monomers. The biological imaging and drug delivery performance of HAp-poly(MPC-IA) nanorods were examined to evaluate their potential for biomedical applications. Results suggested that hydrophilic HAp-poly(MPC-IA) nanorods can be successfully prepared. More importantly, the HAp-poly(MPC-IA) exhibited excellent water dispersibility, desirable biocompatibility and good performance for biological imaging and controlled drug delivery applications. As compared with other controlled living polymerization reactions, the metal free light initiated SI-ATRP displayed many advantages such as easy for handle, mild reaction conditions, toxicity and fluorescence quenching from metal catalysts. Therefore, we believe that this strategy should be a useful and effective strategy for preparation of HAp nanomaterials for biomedical applications.

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Accelerated cell-surface interlocking on plasma polymer-modified porous ceramics

Cited by 25 publications

References 34 publications

Restricted cell functions on micropillars are alleviated by surface-nanocoating with amino groups

Restricted cell functions on micropillars are alleviated by surface-nanocoating with amino groups

Evaluation of the Osteointegration of a Novel Alkali-Treated Implant System <i>In Vivo</i>

Surface grafting of Eu3+ doped luminescent hydroxyapatite nanomaterials through metal free light initiated atom transfer radical polymerization for theranostic applications

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