Isolation of a new antibacterial peptide achromosin from Streptomyces achromogenes subsp. achromogenes based on genome mining

Kaweewan, Issara; Ohnishi‐Kameyama, Mayumi; Kodani, Shinya

doi:10.1038/ja.2016.108

Cited by 17 publications

(9 citation statements)

References 22 publications

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“…Some RiPPs which are produced by Streptomyces have been reported, such as siamycin-I by Streptomyces sp. [ 18 ], cinnamycin by S. cinnamoneus [ 19 ], bottromycin by S. bottropensis [ 20 ], bacteriocin-like by S. scopuliridis [ 21 ], grisemycin by S. griseus [ 22 ], telomestatin by S. anulatus [ 23 ], and archromosin by S. achromogenes [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Streptomyces Isolates Associated with Estuarine Fish Chanos chanos and Profiling of Their Antibacterial Metabolites-Crude-Extract

Kurnianto

Kusumaningrum

Lioe

2020

International Journal of Microbiology

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Streptomyces has been reported as an essential producer of bioactive substances, including antibiotics and other types of antimicrobials. This study investigated antibacterial-producing Streptomyces isolated from the gut of estuarine fish Chanos chanos, emphasizing screening for the producer of peptide-containing antibacterial compounds. Eighteen isolates were found during preliminary screening, in which four isolates showed the best antibacterial activities. Based on the morphological, physiological, and biochemical characterization, as well as 16S rRNA partial sequencing, all of the four isolates belonged to Streptomyces. Three isolates were suspected as novel isolate candidates based on homology presentations and phylogenetic tree analysis. Disk-diffusion assay of the metabolite-crude-extract from the isolates showed broad-spectrum inhibitory activities against Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 10876, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa InaCC B52 with minimum inhibitory concentration and minimum bactericidal concentration ranging from 2.5–10 mg/mL and 5–10 mg/mL, respectively. The highest antibacterial activity with low MIC and MBC values was shown by isolate AIA-10. Qualitative HPLC profiling revealed that the metabolic-crude-extracts showed many peaks with intensive area at 210 and 214 nm, especially from SCA-11 and AIA-10, indicating the presence of peptide groups in the structure of the constituent compound. The results also suggested that crude extracts SCA-11 and AIA-10 had higher hydrophobicity properties than the other extracts. Further characterization of the active compound was needed to find out which compounds were responsible for the antibacterial activity. The results of this study indicated that some Streptomyces isolated from new environmental niches, i.e., gut of estuarine fish Chanos chanos, produce promising peptide-containing bioactive compounds.

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Section: Introductionmentioning

confidence: 99%

Characterization of Streptomyces Isolates Associated with Estuarine Fish Chanos chanos and Profiling of Their Antibacterial Metabolites-Crude-Extract

Kurnianto

Kusumaningrum

Lioe

2020

International Journal of Microbiology

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“…We analyzed known antimicrobial lasso peptides produced by Actinobacteria to expand the chemical space surrounding these natural products. There are currently fifteen lasso peptides produced by Actinobacteria that have reported antimicrobial activity, and twelve have completely annotated BGCs available on the NCBI database (Table S1) [7,26,31,36–44] . By studying biosynthetic diversity within this one phylum, the genetic diversity of the input data is lowered, allowing for the identification of more discrete variations within BGC sequences and phylogenetic relationships.…”

Section: Resultsmentioning

confidence: 99%

Discovery of the Class I Antimicrobial Lasso Peptide Arcumycin

Stariha

McCafferty

2021

ChemBioChem

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Lasso peptides are a structurally diverse superfamily ofconformationally-constrained peptide natural products, of which asubset exhibits broad antimicrobial activity. Although advances inbioinformatics have increased our knowledge of strains harboringthe biosynthetic machinery for lasso peptide production, relatingpeptide sequence to bioactivity remains a continuous challenge.Towards this end, a structure-driven genome mining investigationof Actinobacteria-produced antimicrobial lasso peptides wasperformed to correlate predicted primary structure with antibioticactivity. Bioinformatic evaluation revealed eight putative novelclass I lasso peptide sequences. This subset is predicted topossess antibiotic activity as characterized members of this classhave both broad spectrum and potent activity against Gram positivestrains. Fermentation of one of these hits, StreptomycesNRRL F-5639, resulted in the production of a novel class I lassopeptide, arcumycin, named for the Latin word for bow or arch,arcum. Arcumycin exhibited antibiotic activity against Gram positivebacteria including Bacillus subtilis (4 μg/mL),Staphylococcus aureus (8 μg/mL), and Micrococcus luteus (8μg/mL).Arcumycin treatment of B. subtilis liaI-β-gal promoterfusion reporter strain resulted in upregulation of the system liaRSby the promoter liaI, indicating arcumycin interferes with lipid IIbiosynthesis. Cumulatively, the results illustrate the relationshipbetween phylogenetically related lasso peptides and theirbioactivity as validated through the isolation, structuraldetermination, and evaluation of bioactivity of the novel class Iantimicrobial lasso peptide arcumycin. File list (2)download file view on ChemRxiv Discovery of the Class I Antimicrobial Lasso Peptide Arcu... (1.32 MiB) download file view on ChemRxiv Discovery of the Class I Antimicrobial Lasso Peptide Arcu... (1.33 MiB)

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“…There are currently fifteen lasso peptides produced by Actinobacteria that have reported antimicrobial activity, and twelve have completely annotated BGCs available on the NCBI database (Table S1). [7], [26], [31], [36][37][38][39][40][41][42][43][44] By studying biosynthetic diversity within this one phylum, the genetic diversity of the input data is lowered, allowing for the identification of more discrete variations within BGC sequences and phylogenetic relationships.…”

Section: Resultsmentioning

confidence: 99%

Discovery of the Class I Antimicrobial Lasso Peptide Arcumycin

Stariha

McCafferty²

2021

Preprint

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Lasso peptides are a structurally diverse superfamily ofconformationally-constrained peptide natural products, of which asubset exhibits broad antimicrobial activity. Although advances inbioinformatics have increased our knowledge of strains harboringthe biosynthetic machinery for lasso peptide production, relatingpeptide sequence to bioactivity remains a continuous challenge.Towards this end, a structure-driven genome mining investigationof Actinobacteria-produced antimicrobial lasso peptides wasperformed to correlate predicted primary structure with antibioticactivity. Bioinformatic evaluation revealed eight putative novelclass I lasso peptide sequences. This subset is predicted topossess antibiotic activity as characterized members of this classhave both broad spectrum and potent activity against Gram positivestrains. Fermentation of one of these hits, StreptomycesNRRL F-5639, resulted in the production of a novel class I lassopeptide, arcumycin, named for the Latin word for bow or arch,arcum. Arcumycin exhibited antibiotic activity against Gram positivebacteria including Bacillus subtilis (4 μg/mL),Staphylococcus aureus (8 μg/mL), and Micrococcus luteus (8μg/mL). Arcumycin treatment of B. subtilis liaI-β-gal promoterfusion reporter strain resulted in upregulation of the system liaRSby the promoter liaI, indicating arcumycin interferes with lipid IIbiosynthesis. Cumulatively, the results illustrate the relationshipbetween phylogenetically related lasso peptides and theirbioactivity as validated through the isolation, structuraldetermination, and evaluation of bioactivity of the novel class Iantimicrobial lasso peptide arcumycin.

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Isolation of a new antibacterial peptide achromosin from Streptomyces achromogenes subsp. achromogenes based on genome mining

Abstract: Note Isolation of a new antibacterial peptide achromosin from Streptomyces achromogenes subsp.

Cited by 17 publications

References 22 publications

Characterization of Streptomyces Isolates Associated with Estuarine Fish Chanos chanos and Profiling of Their Antibacterial Metabolites-Crude-Extract

Characterization of Streptomyces Isolates Associated with Estuarine Fish Chanos chanos and Profiling of Their Antibacterial Metabolites-Crude-Extract

Discovery of the Class I Antimicrobial Lasso Peptide Arcumycin

Discovery of the Class I Antimicrobial Lasso Peptide Arcumycin

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