Astrovirus VA1 identified by next-generation sequencing in a nasopharyngeal specimen of a febrile Tanzanian child with acute respiratory disease of unknown etiology

Identification of Viral Signatures Using High-Throughput Sequencing on Blood of Patients With Kawasaki Disease

“…HTS overall performance (e.g., sensitivity and specificity) depends on several parameters such as the sample preparation, the algorithms and the choice of the database used to analyse HTS raw data (27). Therefore, in order to obtain an optimal HTS performance, we used a robust sample preparation protocol and a bioinformatics pipeline both previously published (7,28,29) and benchmarked (30), with an overall sensibility very close to that of real-time (RT-)PCR assays for most of viruses recognized of clinical significance.…”

Novel and classical human astroviruses in stool and cerebrospinal fluid: comprehensive screening in a tertiary care hospital, Switzerland

“… 19 , 20 , 21 , 22 , 23 , 24 Furthermore, we recently detected novel HAstV VA1 in a nasopharyngeal swab specimen from a 13-month-old patient suffering from an acute respiratory disease of unknown etiology. 25 However, the available studies offer limited information on the epidemiology and respective distribution of the novel HAstV known to infect humans. The current routine molecular assays only target the classical HAstV serotypes 1–8, and in the absence of appropriate diagnostic tools, novel HAstV remain largely underdiagnosed.…”

Astrovirus in White-Tailed Deer, United States, 2018

“…Astroviruses cause diarrhea and neurologic diseases in mammals and a spectrum of diseases, including diarrhea, hepatitis, and nephritis, in birds (2). Astrovirus is associated with respiratory disease in humans, cattle, and pigs (3)(4)(5) and has also been found in fecal samples from roe deer with gastrointestinal illness in Denmark (6). Whether astrovirus circulates in other species of deer remains unclear.…”