Evaluation of a blood-specific DNA methylated region and trial for allele-specific blood identification from mixed body fluid DNA

Watanabe, Ken; Akutsu, Tomoko; Takamura, Ayari; Sakurada, Koichi

doi:10.1016/j.legalmed.2016.08.004

Cited by 17 publications

(7 citation statements)

References 30 publications

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“…We then analyzed these regions using DNA from saliva (n = 12), blood (n = 9), semen (n = 9), and vaginal swab (n = 9) samples, which were not included in the pooled samples used for the genome-wide analysis. We used 2 or 5 µL of approximately 20 µL of the eluate after bisulfite treatment of 20 ng for PCR amplification, as we previously could detect specific methylation of semen and blood CpG markers using the same amount in bisulfite PCR-based methods [15,16,22]. As a result of MPS-based bisulfite sequencing, we obtained more than 1000 mapped reads for each sample except one vaginal sample.…”

Section: Searching For Saliva-specific Cpgs In Each Regionmentioning

confidence: 99%

“…Because the differences in methylation ratios of saliva CpG markers are moderate between the target and other body fluids, the presence of a large amount of non-saliva DNA can reduce these ratios to background levels. To overcome the same potential problem with a blood methylation marker, we previously demonstrated a strategy for mixture analysis using blood-specific methylated CpGs and their neighboring single nucleotide polymorphism (SNP) [15]. This method, herein called individual-specific body fluid identification, analyzes the methylation status of CpGs and the allelic patterns of nearby SNPs by cloning-based bisulfite sequencing to detect each body fluid-specific methylation status in the target person's DNA (Fig.…”

Section: Introductionmentioning

confidence: 99%

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A new approach for forensic analysis of saliva-containing body fluid mixtures based on SNPs and methylation patterns of nearby CpGs

Watanabe

Taniguchi

Toyomane

et al. 2022

Forensic Science International: Genetics

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Section: Searching For Saliva-specific Cpgs In Each Regionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A new approach for forensic analysis of saliva-containing body fluid mixtures based on SNPs and methylation patterns of nearby CpGs

Watanabe

Taniguchi

Toyomane

et al. 2022

Forensic Science International: Genetics

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“…One of the main tasks of forensic odontology is the identification of unidentified human remains using dental treatment marks (dental prosthesis and filling, etc) 1‐5 . Other scientific methods for body identification include DNA typing and fingerprinting 6‐11 . Forensic dental identification is not only effective for recently unidentified human remains but is also useful when mass disasters occur and cause numerous deaths.…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3][4][5] Other scientific methods for body identification include DNA typing and fingerprinting. [6][7][8][9][10][11] Forensic dental identification is not only effective for recently unidentified human remains but is also useful when mass disasters occur and cause numerous deaths. Therefore, the Disaster Victim Identification (DVI) team has been organized to promptly respond to disasters in various parts of the world.…”

Section: Introductionmentioning

confidence: 99%

Survey of dental hygienists' and dental hygienist students' attitudes towards body identification tasks of unidentified human remains

Ishikawa

Yamasawa

Kitamura

et al. 2020

Int J Dental Hygiene

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Objective Dental findings are used to identify unidentified human remains. The post‐mortem records, necessary for the appraisal of dental findings, are created by the dentist; however, a system for dental hygienists specializing in these tasks to utilize has not yet been considered. Methods This study was directed at both students at a dental hygienist school and dental hygienists. We conducted an awareness survey on their willingness to participate in these tasks, as well as the need for education regarding body identification tasks. Results The results showed that students and dental hygienists are highly interested in body identification tasks and highly motivated to participate. Numerous participants preferred to take courses regarding basic knowledge in both lecture and practical training settings to acquire the knowledge necessary for performing these tasks. Conclusions It is necessary to consider the curriculum content and timing of lectures and practices related to body identification tasks. Participation limited to the creation of post‐mortem records, except for the creation of antemortem records requiring diagnosis and treatment knowledge and the matching task of antemortem records and post‐mortem records, should be considered to prevent the tasks of dental hygienists from excessively expanding. If dental hygienists train properly, intraoral findings records can be drawn up by a team consisting of 1 dentist and 1 dental hygienist. Therefore, it is expected that the use of forensic dental identification, which is a relatively quick identification method, will be further improved.

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“…However, in most of the studies performed previously 6 – 8 , 10 – 17 , 19 , 20 , the research object has been limited to blood samples. DNA methylation profiles can differ depending on the cell type from which the DNA is derived 25 – 27 . Therefore, an age prediction model established from blood DNA may not be applicable for DNA derived from other biological fluids.…”

Section: Introductionmentioning

confidence: 99%

Forensic age prediction for saliva samples using methylation-sensitive high resolution melting: exploratory application for cigarette butts

Hamano

Manabe

Morimoto

et al. 2017

Sci Rep

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There is high demand for forensic age prediction in actual crime investigations. In this study, a novel age prediction model for saliva samples using methylation-sensitive high resolution melting (MS-HRM) was developed. The methylation profiles of ELOVL2 and EDARADD showed high correlations with age and were used to predict age with support vector regression. ELOVL2 was first reported as an age predictive marker for saliva samples. The prediction model showed high accuracy with a mean absolute deviation (MAD) from chronological age of 5.96 years among 197 training samples. The model was further validated with an additional 50 test samples (MAD = 6.25). In addition, the age prediction model was applied to saliva extracted from seven cigarette butts, as in an actual crime scene. The MAD (7.65 years) for these samples was slightly higher than that of intact saliva samples. A smoking habit or the ingredients of cigarettes themselves did not significantly affect the prediction model and could be ignored. MS-HRM provides a quick (2 hours) and cost-effective (95% decreased compared to that of DNA chips) method of analysis. Thus, this study may provide a novel strategy for predicting the age of a person of interest in actual crime scene investigations.

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Evaluation of a blood-specific DNA methylated region and trial for allele-specific blood identification from mixed body fluid DNA

Cited by 17 publications

References 30 publications

A new approach for forensic analysis of saliva-containing body fluid mixtures based on SNPs and methylation patterns of nearby CpGs

A new approach for forensic analysis of saliva-containing body fluid mixtures based on SNPs and methylation patterns of nearby CpGs

Survey of dental hygienists' and dental hygienist students' attitudes towards body identification tasks of unidentified human remains

Forensic age prediction for saliva samples using methylation-sensitive high resolution melting: exploratory application for cigarette butts

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