Silver enhanced ratiometric nanosensor based on two adjustable Fluorescence Resonance Energy Transfer modes for quantitative protein sensing

Li, Hui; Zhao, Yaju; Chen, Zhu; Xu, Danke

doi:10.1016/j.bios.2016.08.075

Cited by 31 publications

(7 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Excitation wavelength was set at 488 ± 20 nm, and emission wavelength was recorded at 520 ± 20 nm. Ag 10 NPs involved in this work were synthesized, purified, and characterized by the assay previously reported by Li . The preparation and characterization of Ag 10 NPs are shown in Figure S1 (Supporting Information) with 50.3 ± 4.0 nm diameter.…”

Section: Methodsmentioning

confidence: 99%

Bivalent Aptasensor Based on Silver-Enhanced Fluorescence Polarization for Rapid Detection of Lactoferrin in Milk

Zhu

Jia

et al. 2017

Anal. Chem.

Self Cite

View full text Add to dashboard Cite

Here we report a novel type of bivalent aptasensor based on silver-enhanced fluorescence polarization (FP) for detection of lactoferrin (Lac) in milk powder with high sensitivity and specificity. The novel two split aptamers were obtained from the aptamer reported in our previous SELEX (systematic evolution of ligands by exponential enrichment) selection, and their minimal structural units were optimized on the basis of their affinity and specificity. Also, dual binding sites of split aptamers were verified. The bivalent aptamers were modified to be linked with signal-molecule fluorescein isothiocyanate (FITC) and enhancer silver decahedral nanoparticles (AgNPs). The split aptamers could bind to different sites of Lac and assemble into a split-aptamers-target complex, narrowing the distance between AgNPs and FITC dye. As a result, AgNPs could produce a mass-augmented and metal-enhanced fluorescence (MEF) effect. In general, ternary amplification based on AgNPs, split aptamers, and the MEF effect all contributed to the significant increase of FP values. It was proved that the sensitivity of this assay was about 3 orders of magnitude over traditional aptamer-based homogeneous assays with a detection limit of 1.25 pM. Furthermore, this design was examined by actual milk powder with rapid and high-throughout detection.

show abstract

Section: Methodsmentioning

confidence: 99%

Bivalent Aptasensor Based on Silver-Enhanced Fluorescence Polarization for Rapid Detection of Lactoferrin in Milk

Zhu

Jia

et al. 2017

Anal. Chem.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The limit of detection (LOD) at a ratio of signal-to-noise is estimated to be 0.088 pg mL –1 . As shown in Table S1, this LOD value is lower than that of most colorimetric ,− and even many electrochemical − and fluorescence , bioassay approaches previously reported for PDGF-BB analysis. The ultrahigh sensitivity should be ascribed to the great signal amplification of the proximity binding-triggered assembly of two MNAzymes for the catalytic release of a large amount of G-DNAzymes.…”

mentioning

confidence: 70%

Proximity Binding-Triggered Assembly of Two MNAzymes for Catalyzed Release of G-Quadruplex DNAzymes and an Ultrasensitive Homogeneous Bioassay of Platelet-Derived Growth Factor

Xie

Niu

et al. 2019

Anal. Chem.

View full text Add to dashboard Cite

When the target biorecognition-triggered assembly of two Mg2+-dependent DNAzymes (MNAzymes) is employed for dually catalytic release of peroxidase-mimicking G-quadruplex DNAzymes (G-DNAzymes), this work develops a novel homogeneous colorimetric method for an ultrasensitive bioassay of platelet-derived growth factor-BB (PDGF-BB). The first MNAzyme assembly is realized through a highly specific aptamer biorecognition-driven proximity ligation reaction. Its catalytic cleavage toward the two designed hairpin substrates not only releases a large amount of G-DNAzymes for colorimetric signal transduction but also enables the spontaneous assembly of another MNAzyme for signal amplification. This leads to the successful detection of PDGF-BB in a wide linear range from 2.0 pg mL–1 to 20 ng mL–1 with a very low detection down to 0.088 pg mL–1. As the whole reactions including aptamer biorecognitions, DNA hybridizations, and catalytic cleavages of MNAzymes are conducted in a homogeneous solution, this method has very simple manipulations and also has high repeatability. In addition, the high specificity of the aptamer biorecognition-triggered signal transduction decides the excellent selectivity of the method. This bioassay does not require an expensive instrument and nucleic acid labeling for signal readout or any nanomaterial, enzyme, or nuclease for signal amplification. Thus, it displays an extensive potential for clinical diagnostic applications.

show abstract

“…Additionally, several studies have focused on AgNP-enhanced FRET sensors for no-wash detection of cancer protein biomarkers. − In these sensing systems, AgNPs were used as a framework to construct MEF-FRET double models, where the presence of target would trigger the dissociation of the MEF-FRET structure, thus disrupting the FRET response. More recently, Li et al . displayed a similar AgNP-enhanced FRET nanosensor for quantitative detection of PDGF in solution through coupling AgNP-based MEF with two FRET modes.…”

Section: Nanoparticles As Enhancersmentioning

confidence: 99%

Nanotechnology-Enhanced No-Wash Biosensors for in Vitro Diagnostics of Cancer

et al. 2017

View full text Add to dashboard Cite

In vitro biosensors have been an integral component for early diagnosis of cancer in the clinic. Among them, no-wash biosensors, which only depend on the simple mixing of the signal generating probes and the sample solution without additional washing and separation steps, have been found to be particularly attractive. The outstanding advantages of facile, convenient, and rapid response of no-wash biosensors are especially suitable for point-of-care testing (POCT). One fast-growing field of no-wash biosensor design involves the usage of nanomaterials as signal amplification carriers or direct signal generating elements. The analytical capacity of no-wash biosensors with respect to sensitivity or limit of detection, specificity, stability, and multiplexing detection capacity is largely improved because of their large surface area, excellent optical, electrical, catalytic, and magnetic properties. This review provides a comprehensive overview of various nanomaterial-enhanced no-wash biosensing technologies and focuses on the analysis of the underlying mechanism of these technologies applied for the early detection of cancer biomarkers ranging from small molecules to proteins, and even whole cancerous cells. Representative examples are selected to demonstrate the proof-of-concept with promising applications for in vitro diagnostics of cancer. Finally, a brief discussion of common unresolved issues and a perspective outlook on the field are provided.

show abstract

Silver enhanced ratiometric nanosensor based on two adjustable Fluorescence Resonance Energy Transfer modes for quantitative protein sensing

Cited by 31 publications

References 32 publications

Bivalent Aptasensor Based on Silver-Enhanced Fluorescence Polarization for Rapid Detection of Lactoferrin in Milk

Bivalent Aptasensor Based on Silver-Enhanced Fluorescence Polarization for Rapid Detection of Lactoferrin in Milk

Proximity Binding-Triggered Assembly of Two MNAzymes for Catalyzed Release of G-Quadruplex DNAzymes and an Ultrasensitive Homogeneous Bioassay of Platelet-Derived Growth Factor

Nanotechnology-Enhanced No-Wash Biosensors for in Vitro Diagnostics of Cancer

Contact Info

Product

Resources

About