Draft genome sequence of Pseudomonas moraviensis strain Devor implicates metabolic versatility and bioremediation potential

Miller, Neil T.; Fuller, Danny; Couger, Matthew Brian; Bagazinski, Mark; Boyne, Philip J.; Devor, Robert C.; Hanafy, Radwa A.; Budd, Connie; French, Donald P.; Hoff, Wouter D.; Youssef, Noha H.

doi:10.1016/j.gdata.2016.08.004

Cited by 12 publications

(4 citation statements)

References 27 publications

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“…It is used as a BCA and is applied as a seed treatment to suppress late root rot in sugar beet (Zachow et al, 2010). The other antagonistic bacterium, BCA12, belongs to the same clade as P. moraviensis which has been reported as a soil bacterium implicated in the suppression of Rhizoctonia diseases of potato (Tvrzová et al, 2006;Mrabet et al, 2013;Miller et al, 2016). However, the in vitro assay conducted in the current study indicated that BCA12 was less inhibitory compared to the other nine BCAs from the P. poae group.…”

Section: Discussionmentioning

confidence: 99%

Diversity Profiling of Grapevine Microbial Endosphere and Antagonistic Potential of Endophytic Pseudomonas Against Grapevine Trunk Diseases

et al. 2020

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Grapevine trunk diseases (GTDs) are a serious problem of grapevines worldwide. The microbiota of the grapevine endosphere comprises prokaryotic and eukaryotic endophytes, which may form varied relationships with the host plant from symbiotic to pathogenic. To explore the interaction between grapevine endophytic bacteria and GTDs, the endomicrobiome associated with grapevine wood was characterized using next-generation Illumina sequencing. Wood samples were collected from grapevine trunks with and without external symptoms of GTD (cankers) from two vineyards in the Hunter Valley and Hilltops, NSW, Australia and metagenomic characterization of the endophytic community was conducted using the 16S rRNA gene (341F/806R) and ITS (1F/2R) sequences. Among the important GTD pathogens, Phaeomoniella, Phaeoacremonium, Diplodia and Cryptovalsa species were found to be abundant in both symptomatic and asymptomatic grapevines from both vineyards. Eutypa lata and Neofusicoccum parvum, two important GTD pathogens, were detected in low numbers in Hilltops and the Hunter Valley, respectively. Interestingly, Pseudomonas dominated the bacterial community in canker-free grapevine tissues in both locations, comprising 56-74% of the total bacterial population. In contrast, the Pseudomonas population in grapevines with cankers was significantly lower, representing 29 and 2% of the bacterial community in Hilltops and the Hunter Valley, respectively. The presence of Pseudomonas in healthy grapevine tissues indicates its ability to colonize and survive in the grapevine. The potential of Pseudomonas spp. as biocontrol agents against GTD pathogens was also explored. Dual culture tests with isolated fluorescent Pseudomonas against mycelial discs of nine Botryosphaeria dieback, three Eutypa dieback, and two Esca/Petri disease pathogens, revealed antagonistic activity for 10 Pseudomonas strains. These results suggest the potential of Pseudomonas species from grapevine wood to be used as biocontrol agents to manage certain GTD pathogens.

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Section: Discussionmentioning

confidence: 99%

Diversity Profiling of Grapevine Microbial Endosphere and Antagonistic Potential of Endophytic Pseudomonas Against Grapevine Trunk Diseases

et al. 2020

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“…Only one strain was tested for pathogeny and was negative. P. moraviensis (Tvrzovà et al 2006 ) is an ubiquist species with high metabolic versatility and bioremediation potential (Miller et al 2016 ). It is also considered as a PGPR (Plant Growth-Promoting Rhizobacteria) (Hassan et al 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia

et al. 2020

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The damages observed in Tunisian citrus orchards have prompted studies on the Pseudomonas spp. responsible for blast and black pit. Prospective orchards between 2015 and 2017 showed that the diseases rapidly spread geographically and to new cultivars. A screening of Pseudomonas spp. isolated from symptomatic trees revealed their wide diversity according to phylogenetic analysis of their housekeeping rpoD and cts genes. The majority of strains were affiliated to Pseudomonas syringae pv. syringae (Phylogroup PG02b), previously described in Tunisia. However, they exhibited various BOX-PCR fingerprints and were not clonal. This work demonstrated, for the first time in Tunisia, the involvement of Pseudomonas cerasi (PG02a) and Pseudomonas congelans (PG02c). The latter did not show significant pathogenicity on citrus, but was pathogenic on cantaloupe and active for ice nucleation that could play a role in the disease. A comparative phylogenetic study of citrus pathogens from Iran, Montenegro and Tunisia revealed that P. syringae (PG02b) strains are closely related but again not clonal. Interestingly P. cerasi (PG02a) was isolated in two countries and seems to outspread. However, its role in the diseases is not fully understood and it should be monitored in future studies. The diversity of pathogenic Pseudomonas spp. and the extension of the diseases highlight that they have become complex and synergistic. It opens questions about which factors favor diseases and how to fight against them efficiently and with sustainable means.

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“…Close relatives of Pseudomonas, Massilia, and Ralstonia ESVs are found in a remarkable diversity of conditions and environments, including spacecraft assembly rooms, soil, water, air, guts, and health clinics (Miller et al, 2016 ; Nurjadi et al, 2020 ; Narenkumar et al, 2021 ). Most are mesophilic, though a few are known to be psychrotolerant.…”

Section: Discussionmentioning

confidence: 99%

Earth to Mars: A Protocol for Characterizing Permafrost in the Context of Climate Change as an Analog for Extraplanetary Exploration

et al. 2023

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Permafrost is important from an exobiology and climate change perspective. It serves as an analog for extraplanetary exploration, and it threatens to emit globally significant amounts of greenhouse gases as it thaws due to climate change. Viable microbes survive in Earth's permafrost, slowly metabolizing and transforming organic matter through geologic time. Ancient permafrost microbial communities represent a crucial resource for gaining novel insights into survival strategies adopted by extremotolerant organisms in extraplanetary analogs. We present a proof-of-concept study on ∼22 Kya permafrost to determine the potential for coupling Raman and fluorescence biosignature detection technology from the NASA Mars Perseverance rover with microbial community characterization in frozen soils, which could be expanded to other Earth and off-Earth locations. Besides the well-known utility for biosignature detection and identification, our results indicate that spectral mapping of permafrost could be used to rapidly characterize organic carbon characteristics. Coupled with microbial community analyses, this method has the potential to enhance our understanding of carbon degradation and emissions in thawing permafrost. Further, spectroscopy can be accomplished in situ to mitigate sample transport challenges and in assessing and prioritizing frozen soils for further investigation. This method has broad-range applicability to understanding microbial communities and their associations with biosignatures and soil carbon and mineralogic characteristics relevant to climate science and astrobiology.

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Draft genome sequence of Pseudomonas moraviensis strain Devor implicates metabolic versatility and bioremediation potential

Cited by 12 publications

References 27 publications

Diversity Profiling of Grapevine Microbial Endosphere and Antagonistic Potential of Endophytic Pseudomonas Against Grapevine Trunk Diseases

Diversity Profiling of Grapevine Microbial Endosphere and Antagonistic Potential of Endophytic Pseudomonas Against Grapevine Trunk Diseases

Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia

Earth to Mars: A Protocol for Characterizing Permafrost in the Context of Climate Change as an Analog for Extraplanetary Exploration

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