Distribution and activity of the anaerobic methanotrophic community in a nitrogen-fertilized Italian paddy soil

Vaksmaa, Annika; Lüke, Claudia; Alen, Theo van; Valè, Giampiero; Lupotto, Elisabetta; Jetten, Mike S. M.; Ettwig, Katharina F.

doi:10.1093/femsec/fiw181

Cited by 82 publications

(57 citation statements)

References 69 publications

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“…This archaeon, Methanoperedens nitroreducens , is closely related to S‐AOM performing ANME but uses nitrate as the terminal electron acceptor (Arshad et al, ; Haroon et al, ). M. nitroreducens have so far mostly been detected in freshwater habitats similar to those of M. oxyfera , where they coexist, as nitrite produced by M. nitroreducens can be used by M. oxyfera bacteria (Vaksmaa et al, ; Welte et al, ).…”

Section: Microbial Controls On Methane Transformationsmentioning

confidence: 99%

Methane Feedbacks to the Global Climate System in a Warmer World

Dean

Middelburg

Röckmann

et al. 2018

Reviews of Geophysics

426

341

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Methane (CH4) is produced in many natural systems that are vulnerable to change under a warming climate, yet current CH4 budgets, as well as future shifts in CH4 emissions, have high uncertainties. Climate change has the potential to increase CH4 emissions from critical systems such as wetlands, marine and freshwater systems, permafrost, and methane hydrates, through shifts in temperature, hydrology, vegetation, landscape disturbance, and sea level rise. Increased CH4 emissions from these systems would in turn induce further climate change, resulting in a positive climate feedback. Here we synthesize biological, geochemical, and physically focused CH4 climate feedback literature, bringing together the key findings of these disciplines. We discuss environment‐specific feedback processes, including the microbial, physical, and geochemical interlinkages and the timescales on which they operate, and present the current state of knowledge of CH4 climate feedbacks in the immediate and distant future. The important linkages between microbial activity and climate warming are discussed with the aim to better constrain the sensitivity of the CH4 cycle to future climate predictions. We determine that wetlands will form the majority of the CH4 climate feedback up to 2100. Beyond this timescale, CH4 emissions from marine and freshwater systems and permafrost environments could become more important. Significant CH4 emissions to the atmosphere from the dissociation of methane hydrates are not expected in the near future. Our key findings highlight the importance of quantifying whether CH4 consumption can counterbalance CH4 production under future climate scenarios.

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Section: Microbial Controls On Methane Transformationsmentioning

confidence: 99%

Methane Feedbacks to the Global Climate System in a Warmer World

Dean

Middelburg

Röckmann

et al. 2018

Reviews of Geophysics

426

341

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“…Nitrite-dependent methane oxidizers have been cocultured with anammox bacteria (72) and naturally cooccur in various anaerobic environments (73)(74)(75). Organisms linked to anaerobic methane oxidation with nitrate or nitrite, such as anaerobic methane-oxidizing archaea (ANME) or NC10-type organisms (72,(76)(77)(78), were not identified by 16S sequencing; however, this dynamic needs further investigation in our system.…”

Section: Figmentioning

confidence: 99%

Sulfide-Induced Dissimilatory Nitrate Reduction to Ammonium Supports Anaerobic Ammonium Oxidation (Anammox) in an Open-Water Unit Process Wetland

Jones

Jasper

Sedlak

et al. 2017

Appl Environ Microbiol

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Open-water unit process wetlands host a benthic diatomaceous and bacterial assemblage capable of nitrate removal from treated municipal wastewater with unexpected contributions from anammox processes. In exploring mechanistic drivers of anammox, 16S rRNA gene sequencing profiles of the biomat revealed significant microbial community shifts along the flow path and with depth. Notably, there was an increasing abundance of sulfate reducers (Desulfococcus and other Deltaproteobacteria) and anammox microorganisms (Brocadiaceae) with depth. Pore water profiles demonstrated that nitrate and sulfate concentrations exhibited a commensurate decrease with biomat depth accompanied by the accumulation of ammonium. Quantitative PCR targeting the anammox hydrazine synthase gene, hzsA, revealed a 3-fold increase in abundance with biomat depth as well as a 2-fold increase in the sulfate reductase gene, dsrA. These microbial and geochemical trends were most pronounced in proximity to the influent region of the wetland where the biomat was thickest and influent nitrate concentrations were highest. While direct genetic queries for dissimilatory nitrate reduction to ammonium (DNRA) microorganisms proved unsuccessful, an increasing depth-dependent dominance of Gammaproteobacteria and diatoms that have previously been functionally linked to DNRA was observed. To further explore this potential, a series of microcosms containing field-derived biomat material confirmed the ability of the community to produce sulfide and reduce nitrate; however, significant ammonium production was observed only in the presence of hydrogen sulfide. Collectively, these results suggest that biogenic sulfide induces DNRA, which in turn can explain the requisite coproduction of ammonium and nitrite from nitrified effluent necessary to sustain the anammox community.IMPORTANCE This study aims to increase understanding of why and how anammox is occurring in an engineered wetland with limited exogenous contributions of ammonium and nitrite. In doing so, the study has implications for how geochemical parameters could potentially be leveraged to impact nutrient cycling and attenuation during the operation of treatment wetlands. The work also contributes to ongoing discussions about biogeochemical signatures surrounding anammox processes and enhances our understanding of the contributions of anammox processes in freshwater environments.KEYWORDS DNRA, anammox, nitrogen cycle, sulfide, water treatment, wetlands C onstructed freshwater wetlands that receive wastewater effluent offer an opportunity to gain insight into the biological nutrient cycling process because they are subject to comparatively high nutrient loading rates and are optimized for steady-state

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“…So far they have been found in freshwater canals [31], soils and rice paddy fields [34–36], lakes and rivers [35], and wastewater treatment plants [33]. In situ AOM activity of ANME-2d was determined recently for the first time [36]. More ANME phylotypes in different environments and possibly new archaeal clades involved in AOM may yet have to be discovered.…”

Section: Introductionmentioning

confidence: 99%

Reverse Methanogenesis and Respiration in Methanotrophic Archaea

Timmers

Welte

Koehorst

et al. 2017

Archaea

260

196

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Anaerobic oxidation of methane (AOM) is catalyzed by anaerobic methane-oxidizing archaea (ANME) via a reverse and modified methanogenesis pathway. Methanogens can also reverse the methanogenesis pathway to oxidize methane, but only during net methane production (i.e., “trace methane oxidation”). In turn, ANME can produce methane, but only during net methane oxidation (i.e., enzymatic back flux). Net AOM is exergonic when coupled to an external electron acceptor such as sulfate (ANME-1, ANME-2abc, and ANME-3), nitrate (ANME-2d), or metal (oxides). In this review, the reversibility of the methanogenesis pathway and essential differences between ANME and methanogens are described by combining published information with domain based (meta)genome comparison of archaeal methanotrophs and selected archaea. These differences include abundances and special structure of methyl coenzyme M reductase and of multiheme cytochromes and the presence of menaquinones or methanophenazines. ANME-2a and ANME-2d can use electron acceptors other than sulfate or nitrate for AOM, respectively. Environmental studies suggest that ANME-2d are also involved in sulfate-dependent AOM. ANME-1 seem to use a different mechanism for disposal of electrons and possibly are less versatile in electron acceptors use than ANME-2. Future research will shed light on the molecular basis of reversal of the methanogenic pathway and electron transfer in different ANME types.

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Distribution and activity of the anaerobic methanotrophic community in a nitrogen-fertilized Italian paddy soil

Cited by 82 publications

References 69 publications

Methane Feedbacks to the Global Climate System in a Warmer World

Methane Feedbacks to the Global Climate System in a Warmer World

Sulfide-Induced Dissimilatory Nitrate Reduction to Ammonium Supports Anaerobic Ammonium Oxidation (Anammox) in an Open-Water Unit Process Wetland

Reverse Methanogenesis and Respiration in Methanotrophic Archaea

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